Human CCL22/MDC Quantikine ELISA Kit

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Human CCL22/MDC ELISA Standard Curve
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Human CCL22/MDC Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity
62.5 pg/mL
Assay Range
125.0 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human MDC
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human MDC Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human MDC in cell culture supernates, serum, or plasma. It contains E. coli-expressed recombinant human MDC and has been shown to accurately quantitate the recombinant factor. Results obtained using naturally expressed human MDC showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally expressed human MDC.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 284 452 821 276 440 810
Standard Deviation 12 9.7 24 22.2 27.8 42.8
CV% 4.2 2.1 2.9 8 6.3 5.3

Recovery

The recovery of MDC spiked to three different levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 98 90-110

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of MDC were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.\line
Human CCL22/MDC ELISA Linearity

Data Examples

Human CCL22/MDC ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL22/MDC

Macrophage-derived chemokine (MDC), also named stimulated T cell chemotactic protein (STCP-1) and ABCD-1, and now designated as CCL22, is a CC chemokine initially isolated from clones of monocyte-derived macrophages. At the amino acid sequence level, MDC shows less than 35% identity to other CC chemokine family members. Human MDC is expressed in dendritic cells, macrophages and activated monocytes. In addition, MDC expression is detected in thymus, lymph node and appendix tissues. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity.

Entrez Gene IDs:
6367 (Human); 20299 (Mouse)
Alternate Names:
A-152E5.1; ABCD-1; CC chemokine STCP-1; C-C motif chemokine 22; CCL22; chemokine (C-C motif) ligand 22; DC/B-CK; Macrophage-derived chemokine; MDC; MDCStimulated T-cell chemotactic protein 1; MGC34554; SCYA22MDC(1-69); small inducible cytokine A22; small inducible cytokine subfamily A (Cys-Cys), member 22; Small-inducible cytokine A22; STCP-1; stimulated T cell chemotactic protein 1
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

It is recommended that all samples and standards be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at 2-8 °C for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL cold Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at 2-8 °C for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human CCL22/MDC Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. EBV+ tumors exploit tumor cell-intrinsic and -extrinsic mechanisms to produce regulatory T cell-recruiting chemokines CCL17 and CCL22
    Authors: A Jorapur, LA Marshall, S Jacobson, M Xu, S Marubayash, M Zibinsky, DX Hu, O Robles, JJ Jackson, V Baloche, P Busson, D Wustrow, DG Brockstedt, O Talay, PD Kassner, G Cutler
    PloS Pathogens, 2022;18(1):e1010200.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. NF&kapppaB-Activated COX2/PGE2/EP4 Axis Controls the Magnitude and Selectivity of BCG-Induced Inflammation in Human Bladder Cancer Tissues
    Authors: OM Ibrahim, PH Basse, W Jiang, K Guru, G Chatta, P Kalinski
    Cancers, 2021;13(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Higher CCL22+ Cell Infiltration is Associated with Poor Prognosis in Cervical Cancer Patients
    Authors: Q Wang, E Schmoeckel, BP Kost, C Kuhn, A Vattai, T Vilsmaier, S Mahner, D Mayr, U Jeschke, HH Heidegger
    Cancers (Basel), 2019;11(12):.
    Species: Human
    Sample Types: Serum
  4. Mitochondrial fission-induced mtDNA stress promotes tumor-associated macrophage infiltration and HCC progression
    Authors: D Bao, J Zhao, X Zhou, Q Yang, Y Chen, J Zhu, P Yuan, J Yang, T Qin, S Wan, J Xing
    Oncogene, 2019;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Changes in the Anti-Allergic Activities of Sesame by Bioconversion
    Authors: TD Jung, SI Choi, SH Choi, BY Cho, WS Sim,, SJ Lee, SJ Park, DB Kim, YC Kim, JH Lee, OH Lee
    Nutrients, 2018;10(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Case-control, exploratory study of cerebrospinal fluid chemokines/cytokines and lymphocyte subsets in childhood Tourette syndrome with positive streptococcal markers
    Authors: MR Pranzatell, ED Tate, TJ Allison
    Cytokine, 2017;96(0):49-53.
    Species: Human
    Sample Types: Serum
  7. High salt primes a specific activation state of macrophages, M(Na).
    Authors: Zhang W, Zheng X, DU L, Sun J, Shen Z, Shi C, Sun S, Zhang Z, Chen X, Qin M, Liu X, Tao J, Jia L, Fan H, Zhou B, Yu Y, Ying H, Hui L, Liu X, Yi X, Liu X, Zhang L, Duan S
    Cell Res, 2015;25(8):893-910.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Expansion of activated regulatory T cells by myeloid-specific chemokines via an alternative pathway in CSF of bacterial meningitis patients.
    Authors: Shi G, Han J, Liu G, Hao Y, Ma Y, Li T, Wu X, Zhang H, Liu Y, Wang B, Kong Y, Zhou J, Zeng H
    Eur J Immunol, 2014;44(2):420-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. BAFF/APRIL system in pediatric OMS: relation to severity, neuroinflammation, and immunotherapy.
    Authors: Pranzatelli, Michael, Tate, Elizabet, McGee, Nathan R, Travelstead, Anna L, Colliver, Jerry A, Ness, Jayne M, Ransohoff, Richard
    J Neuroinflammation, 2013;10(0):10.
    Species: Human
    Sample Types: Serum
  10. Regulatory T cells suppress T cell activation at the pathologic site of human visceral leishmaniasis.
    Authors: Rai AK, Thakur CP, Singh A
    PLoS ONE, 2012;7(2):e31551.
    Species: Human
    Sample Types: Serum
  11. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
    Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
    J. Immunol., 2010;184(6):2886-98.
  12. NOD1 contributes to mouse host defense against Helicobacter pylori via induction of type I IFN and activation of the ISGF3 signaling pathway.
    Authors: Watanabe T, Asano N, Fichtner-Feigl S, Gorelick PL, Tsuji Y, Matsumoto Y, Chiba T, Fuss IJ, Kitani A, Strober W
    J. Clin. Invest., 2010;120(5):1645-62.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells.
    Authors: Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A
    J. Exp. Med., 2009;206(1):169-81.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. The imbalance in serum concentration of Th-1- and Th-2-derived chemokines as one of the factors involved in pathogenesis of atopic dermatitis.
    Authors: Narbutt J, Lesiak A, Sysa-Jedrzeiowska A, Zakrzewski M, Bogaczewicz J, Stelmach I, Kuna P
    Mediators Inflamm., 2009;2009(0):269541.
    Species: Human
    Sample Types: Serum
  15. In vitro induction of a dendritic cell phenotype in primary human acute myelogenous leukemia (AML) blasts alters the chemokine release profile and increases the levels of T cell chemotactic CCL17 and CCL22.
    Authors: Olsnes AM, Ryningen A, Ersvaer E, Bruserud Ø
    J. Interferon Cytokine Res., 2008;28(5):297-310.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Chemokine production and chemokine receptor expression by human glioma cells: role of CXCL10 in tumour cell proliferation.
    Authors: Maru SV, Holloway KA, Flynn G, Lancashire CL, Loughlin AJ, Male DK, Romero IA
    J. Neuroimmunol., 2008;199(1):35-45.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Specific recruitment of regulatory T cells into the CSF in lymphomatous and carcinomatous meningitis.
    Authors: Haas J, Schopp L, Storch-Hagenlocher B, Fritzsching B, Jacobi C, Milkova L, Fritz B, Schwarz A, Suri-Payer E, Hensel M, Wildemann B
    Blood, 2007;111(2):761-6.
    Species: Human
    Sample Types: CSF
  18. Protection against inflammation- and autoantibody-caused fetal loss by the chemokine decoy receptor D6.
    Authors: Martinez de la Torre Y, Buracchi C, Borroni EM, Dupor J, Bonecchi R, Nebuloni M, Pasqualini F, Doni A, Lauri E, Agostinis C, Bulla R, Cook DN, Haribabu B, Meroni P, Rukavina D, Vago L, Tedesco F, Vecchi A, Lira SA, Locati M, Mantovani A
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(7):2319-24.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. CD16+ monocyte-derived macrophages activate resting T cells for HIV infection by producing CCR3 and CCR4 ligands.
    Authors: Ancuta P, Autissier P, Wurcel A, Zaman T, Stone D, Gabuzda D
    J. Immunol., 2006;176(10):5760-71.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Role of platelet-derived growth factor and transforming growth factor beta1 the in the regulation of metalloproteinase expressions.
    Authors: Borrelli V, di Marzo L, Sapienza P, Colasanti M, Moroni E, Cavallaro A
    Surgery, 2006;140(3):454-63.
    Species: Human
    Sample Types: Plasma
  21. Chemokines indicate allergic bronchopulmonary aspergillosis in patients with cystic fibrosis.
    Authors: Hartl D, Latzin P, Zissel G, Krane M, Krauss-Etschmann S, Griese M
    Am. J. Respir. Crit. Care Med., 2006;173(12):1370-6.
    Species: Human
    Sample Types: Serum
  22. Increased macrophage-derived chemokine in exhaled breath condensate and plasma from children with asthma.
    Authors: Leung TF, Wong GW, Ko FW, Lam CW, Fok TF
    Clin. Exp. Allergy, 2004;34(5):786-91.
    Species: Human
    Sample Types: Plasma
  23. Serum levels of cutaneous T-cell attracting chemokine (CTACK) as a laboratory marker of the severity of atopic dermatitis in children.
    Authors: Hon KL, Leung TF, Ma KC, Li AM, Wong Y, Fok TF
    Clin. Exp. Dermatol., 2004;29(3):293-6.
    Species: Human
    Sample Types: Serum
  24. Enhanced monocyte chemoattractant protein-3/CC chemokine ligand-7 in usual interstitial pneumonia.
    Authors: Choi ES, Jakubzick C, Carpenter KJ, Kunkel SL, Evanoff H, Martinez FJ, Flaherty KR, Toews GB, Colby TV, Kazerooni EA, Gross BH, Travis WD, Hogaboam CM
    Am. J. Respir. Crit. Care Med., 2004;170(5):508-15.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Helper T-lymphocyte-related chemokines in healthy newborns.
    Authors: Leung TF, Ng PC, Tam WH, Li CY, Wong E, Ma TP, Lam CW, Fok TF
    Pediatr. Res., 2003;55(2):334-8.
    Species: Human
    Sample Types: Serum
  26. Chemokine receptor CCR4 on CD4+ T cells in juvenile rheumatoid arthritis synovial fluid defines a subset of cells with increased IL-4:IFN-gamma mRNA ratios.
    Authors: Thompson SD, Luyrink LK, Graham TB, Tsoras M, Ryan M, Passo MH, Glass DN
    J. Immunol., 2001;166(11):6899-906.
    Species: Human
    Sample Types: Synovial Fluid
  27. A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases.
    Authors: Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G
    J. Leukoc. Biol., 2001;70(4):617-23.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Clonal Th2 cells associated with chronic hypereosinophilia: TARC-induced CCR4 down-regulation in vivo.
    Authors: de Lavareille A, Roufosse F, Schandene L, Stordeur P, Cogan E, Goldman M
    Eur. J. Immunol., 2001;31(4):1037-46.
    Species: Human
    Sample Types: Serum

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Human CCL22/MDC Quantikine ELISA Kit
By Anonymous on 12/07/2017
Sample Tested: Cell culture supernatant