Human CCL27/CTACK Antibody

Catalog # Availability Size / Price Qty
MAB376-SP
MAB376-100
MAB376-500
Human CCL27/CTACK Antibody in Data
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Product Details
Citations (5)
FAQs
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Reviews (2)

Human CCL27/CTACK Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL27/CTACK in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9/10, 11, 12, 17, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20 is observed.
Source
Monoclonal Mouse IgG1 Clone # 124308
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human CCL27/CTACK
Phe25-Gly112
Accession # NP_006655
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CCL27/CTACK (Catalog # 376-CT)
Immunohistochemistry
8-25 µg/mL
See below
Neutralization
Measured by its ability to neutralize CCL27/CTACK-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR10. The Neutralization Dose (ND50) is typically 0.5-3.0 µg/mL in the presence of 1 µg/mL Recombinant Human CCL27/CTACK.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Neutralization Chemotaxis Induced by CCL27/CTACK and Neutralization by Human CCL27/CTACK Antibody. View Larger

Chemotaxis Induced by CCL27/CTACK and Neutralization by Human CCL27/CTACK Antibody. Recombinant Human CCL27/CTACK (Catalog # 376-CT) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL27/CTACK (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL27/CTACK Monoclonal Antibody (Catalog # MAB376). The ND50is typically 0.5-3.0 µg/mL.

Immunohistochemistry CCL27/CTACK in Human Skin. View Larger

CCL27/CTACK in Human Skin. CCL27/CTACK was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human CCL27/CTACK Monoclonal Antibody (Catalog # MAB376) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL27/CTACK

CCL27, also known as CTACK (cutaneous T cell-attracting chemokine), ALP, ILC, and ESkine, is a member of the CC family of chemokines (1). Mature human CCL27 is an 88 amino acid (aa) protein that shares 57% aa sequence identity with mouse and rat CCL27 (2). It shares 11%‑35% aa sequence identity with other human CC chemokines. An alternately spliced form of mouse CCL27, known as PESKY, is localized to the nucleus and promotes cellular migration (3). CCL27 is constitutively expressed by keratinocytes and is up‑regulated by inflammatory stimuli and in wounded skin (4‑7). CCL27 binds the chemokine receptor CCR10, glycosaminoglycans in the extracellular matrix, sulfated tyrosine residues on PSGL-1, and determinants on the surface of fibroblasts and endothelial cells (5, 7‑9). CCL27 cooperates with CCL17/TARC in inducing the migration of cutaneous lymphocyte antigen (CLA) positive memory T cells to the skin during inflammation (4, 6, 10‑12). Endothelial cell-bound CCL27 can mediate the adhesion of those cells to CLA+ T cells (6). CCL27 also induces the migration of keratinocyte precursors from bone marrow to the skin, thereby promoting wound healing (7). In humans, serum CCL27 levels are elevated and correlate with disease severity in atopic dermatitis, psoriasis vulgaris, and mycosis fungoides (13‑15).

References
  1. Kunkel, L. and E.C. Butcher (2002) Immunity 16:1.
  2. Ishikawa-Mochizuki, I. et al. (1999) FEBS Lett. 460:544.
  3. Gortz, A. et al. (2002) J. Immunol. 169:1387.
  4. Morales, J. et al. (1999) Proc. Natl. Acad. Sci. 96:14470.
  5. Homey, B. et al. (2000) J. Immunol. 164:3465.
  6. Homey, B. et al. (2002) Nat. Med. 8:157.
  7. Inokuma, D. et al. (2006) Stem Cells 24:2810.
  8. Jarmin, D. et al. (2000) J. Immunol. 164:3460.
  9. Hirata, T. et al. (2004) J. Biol. Chem. 279:51775.
  10. Vestergaard, C. et al. (2004) Exp. Dermatol. 13:551.
  11. Reiss, Y. et al. (2001) J. Exp. Med. 194:1541.
  12. Soler, D. et al. (2003) Blood 101:1677.
  13. Kakinuma, T. et al. (2003) J. Allergy Clin. Immunol. 111:592.
  14. Hijnen, D. et al. (2004) J. Allergy Clin. Immunol. 113:334.
  15. Fujita, Y. et al. (2006) Clin. Cancer Res. 12:2670.
Entrez Gene IDs
10850 (Human); 20301 (Mouse); 362505 (Rat)
Alternate Names
ALP; C-C motif chemokine 27; CCL27; chemokine (C-C motif) ligand 27; CTACK; CTACKSmall-inducible cytokine A27; CTAK; cutaneous T-cell attracting chemokine; Cutaneous T-cell-attracting chemokine; ESKINE; IL-11 Ralpha-locus chemokine; ILC; ILCCC chemokine ILC; PESKY; SCYA27IL-11 R-alpha-locus chemokine; skinkine; small inducible cytokine subfamily A (Cys-Cys), member 27

Product Datasheets

Citations for Human CCL27/CTACK Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. CCL27/CCL28-CCR10 CHEMOKINE SIGNALING MEDIATES MIGRATION OF LYMPHATIC ENDOTHELIAL CELLS
    Authors: T Karnezis, R Farnsworth, NC Harris, SP Williams, C Caesar, DJ Byrne, P Herle, ML Macheda, R Shayan, YF Zhang, S Yazar, SJ Takouridis, C Gerard, S Fox, MG Achen, SA Stacker
    Cancer Res., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity
    Authors: Zaidoon Al-Jaderi
    Front Immunol, 2016;7(0):437.
    Species: Human
    Sample Types: Whole Cells
    Applications: Granzyme B Activity
  3. Serum and tissue CTACK/CCL27 chemokine levels in early mycosis fungoides may be correlated with disease-free survival following treatment with interferon alfa and psoralen plus ultraviolet A therapy.
    Authors: Goteri G, Rupoli S, Campanati A, Zizzi A, Picardi P, Cardelli M, Giantomassi F, Canafoglia L, Marchegiani F, Mozzicafreddo G, Brandozzi G, Stramazzotti D, Ganzetti G, Lisa R, Simonetti O, Offidani A, Federici I, Filosa G, Leoni P
    Br. J. Dermatol., 2012;166(5):948-52.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Human basal cell carcinoma is associated with Foxp3+ T cells in a Th2 dominant microenvironment.
    Authors: Kaporis HG, Guttman-Yassky E, Lowes MA, Haider AS, Fuentes-Duculan J, Darabi K, Whynot-Ertelt J, Khatcherian A, Cardinale I, Novitskaya I, Krueger JG, Carucci JA
    J. Invest. Dermatol., 2007;127(10):2391-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Presence of circulating CCR10+ T cells and elevated serum CTACK/CCL27 in the early stage of mycosis fungoides.
    Authors: Sasaki M, Honda A, Furuichi M, Norisugi O
    Clin. Cancer Res., 2006;12(9):2670-5.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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Reviews for Human CCL27/CTACK Antibody

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Human CCL27/CTACK Antibody
By Anonymous on 09/05/2019
Application: ELISA Sample Tested: Serum Species: Human

Human CCL27/CTACK Antibody
By Anonymous on 11/15/2017
Application: ELISA Sample Tested: Plasma Species: Human

This antibody was used in a sandwich ELISA with biotinylated AF376 as detection at a concentration of 50ng/ml.