Human CCL27/CTACK Quantikine ELISA Kit

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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    4.68 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human CTACK
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human CTACK Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CTACK in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human CTACK and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CTACK showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring CTACK.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation4.799.0528.416.121.533.8


The recovery of CTACK spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 91-105
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of CTACK were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CCL27/CTACK Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL27/CTACK

CCL27, also known as Cutaneous T cell-attracting chemokine (CTACK), ALP, ILC and ESkine, is a CC chemokine discovered using expressed sequence tags (EST) in a homology search for novel chemokines. Mature human and mouse CCL27 are 84% similar. Mouse CCL27 shares 37% and 35% amino acid identity with mouse MIP-3 beta and mouse TECK, respectively. CCL27 is predominantly expressed in the skin. The expression is up-regulated by proinflammatory cytokines.

    • Entrez Gene IDs
      10850 (Human); 20301 (Mouse); 362505 (Rat);
    • Alternate Names
      ALP; C-C motif chemokine 27; chemokine (C-C motif) ligand 27; CTACK; CTACKSmall-inducible cytokine A27; CTAK; cutaneous T-cell attracting chemokine; Cutaneous T-cell-attracting chemokine; ESKINE; IL-11 Ralpha-locus chemokine; ILC; ILCCC chemokine ILC; PESKY; SCYA27IL-11 R-alpha-locus chemokine; skinkine; small inducible cytokine subfamily A (Cys-Cys), member 27;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 8 of 8
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    Sample Type
    1. Phenotypic characterization of leukocytes in prenatal human dermis.
      J. Invest. Dermatol., 2012;132(11):2581-92.
      Species: Human
      Sample Type: Cell Culture Supernates
    2. Serum and tissue CTACK/CCL27 chemokine levels in early mycosis fungoides may be correlated with disease-free survival following treatment with interferon alfa and psoralen plus ultraviolet A therapy.
      Authors: Goteri G, Rupoli S, Campanati A, Zizzi A, Picardi P, Cardelli M, Giantomassi F, Canafoglia L, Marchegiani F, Mozzicafreddo G, Brandozzi G, Stramazzotti D, Ganzetti G, Lisa R, Simonetti O, Offidani A, Federici I, Filosa G, Leoni P
      Br. J. Dermatol., 2012;166(5):948-52.
      Species: Human
      Sample Type: Serum
    3. Phenotype of atopic dermatitis subjects with a history of eczema herpeticum.
      Authors: Beck LA, Boguniewicz M, Hata T, Schneider LC, Hanifin J, Gallo R, Paller AS, Lieff S, Reese J, Zaccaro D, Milgrom H, Barnes KC, Leung DY
      J. Allergy Clin. Immunol., 2009;124(2):260-9, 269.e1.
      Species: Human
      Sample Type: Serum
    4. The imbalance in serum concentration of Th-1- and Th-2-derived chemokines as one of the factors involved in pathogenesis of atopic dermatitis.
      Authors: Narbutt J, Lesiak A, Sysa-Jedrzeiowska A, Zakrzewski M, Bogaczewicz J, Stelmach I, Kuna P
      Mediators Inflamm., 2009;2009(0):269541.
      Species: Human
      Sample Type: Serum
    5. Cutting edge: rapid accumulation of epidermal CCL27 in skin-draining lymph nodes following topical application of a contact sensitizer recruits CCR10-expressing T cells.
      Authors: Huang V, Lonsdorf AS, Fang L, Kakinuma T, Lee VC, Cha E, Zhang H, Nagao K, Zaleska M, Olszewski WL, Hwang ST
      J. Immunol., 2008;180(10):6462-6.
      Species: Human
      Sample Type: Serum
    6. Lymphatic precollectors contain a novel, specialized subpopulation of podoplanin low, CCL27-expressing lymphatic endothelial cells.
      Authors: Wick N, Haluza D, Gurnhofer E, Raab I, Kasimir MT, Prinz M, Steiner CW, Reinisch C, Howorka A, Giovanoli P, Buchsbaum S, Krieger S, Tschachler E, Petzelbauer P, Kerjaschki D
      Am. J. Pathol., 2008;173(4):1202-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    7. Presence of circulating CCR10+ T cells and elevated serum CTACK/CCL27 in the early stage of mycosis fungoides.
      Authors: Sasaki M, Honda A, Furuichi M, Norisugi O
      Clin. Cancer Res., 2006;12(9):2670-5.
      Species: Human
      Sample Type: Serum
    8. Serum levels of cutaneous T-cell attracting chemokine (CTACK) as a laboratory marker of the severity of atopic dermatitis in children.
      Authors: Hon KL, Leung TF, Ma KC, Li AM, Wong Y, Fok TF
      Clin. Exp. Dermatol., 2004;29(3):293-6.
      Species: Human
      Sample Type: Serum
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 839 for Human CTACK

    Ctrl QC71
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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine Wash Buffer 1

    ELISA WA126 5
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