Human CCL3/CCL4 Alexa Fluor™ Plus 680‑conjugated Antibody

R&D Systems | Catalog # FAB270AFP680

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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Neutralization, Immunocytochemistry

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 93321
View all CCL3/CCL4 Primary Antibodies »

Product Specifications

Specificity

Detects human CCL3/MIP-1 alpha in direct ELISAs and Western blots. In direct ELISAs, greater than 75% cross-reactivity with recombinant human (rh) CCL4/MIP-1 beta is observed while approximately 30% cross-reactivity with recombinant mouse (rm) CCL3, rmCCL4/MIP-1 beta, and rhCCL14 is observed. No cross-reactivity with rhCCL1, 2, 5, 7, 8, 11, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, rmCCL1, 2, 5, 6, 7, 9/10/MIP-1 gamma, 11, 12, 17, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human CCL3/CCL4 Alexa Fluor™ Plus 680‑conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Neutralization

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CCL3/CCL4

The macrophage inflammatory proteins -1 alpha and -1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP‑1 alpha refers to the products of several independently cloned cDNAs, including LD78, pL78, pAT464, and GOS19. These cDNAs all code for the same human protein that is a homologue of the murine MIP‑1 alpha. Mature MIP-1 alpha and MIP‑1 beta in both human and mouse share approximately 70% homology at the amino acid level. The MIP‑1 proteins are members of the beta (C-C) subfamily of chemokines. Both MIP‑1 alpha and MIP-1 beta are monocyte chemoattractants in vitro. Additionally, the MIP‑1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP‑1 alpha and MIP‑1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. MIP‑1 alpha has also been shown to attract B cells as well as eosinophils. MIP‑1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP‑1 alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. The functional receptor for MIP‑1 alpha has been identified as CCR1 and CCR5.

References

  1. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.

Long Name

CCL3/MIP-1 alpha and CCL4/MIP-1 beta Cross-reactive

Alternate Names

C-C motif chemokine;C-C motif chemokine 3;G0S19-1;LD78ALPHA;MIP1A;MIP-1-alpha;SCYA3

UniProt

P10147

Additional CCL3/CCL4 Products

Product Documents for Human CCL3/CCL4 Alexa Fluor™ Plus 680‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CCL3/CCL4 Alexa Fluor™ Plus 680‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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Protocols

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FAQs

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