Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Development, ELISA Development (Detection)

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all CCL8/MCP-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL8/MCP-2 (R&D Systems, Catalog # 281-CP)
Gln24-Pro99
Accession # P80075

Specificity

Detects human CCL8/MCP-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) HCC‑1, recombinant mouse (rm) MIP-1 alpha, rhMCP-1, and rhMCP-3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human CCL8/MCP‑2 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human CCL8/MCP‑2 (Catalog # 281-CP)

Human CCL8/MCP-2 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human CCL8/MCP‑2 Antibody (Catalog # MAB281)
  • Standard: Recombinant Human CCL8/MCP-2 Protein (Catalog # 281-CP)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using BAF281 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL8/MCP-2

MCP-2 and MCP-3 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. MCP-3 also shares 58% amino acid identity with MCP-2.

Similar to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Alternate Names

MCP-2, MCP2

Entrez Gene IDs

6355 (Human); 20307 (Mouse)

Gene Symbol

CCL8

UniProt

P80075

Additional CCL8/MCP-2 Products

Product Documents for Human CCL8/MCP‑2 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CCL8/MCP‑2 Biotinylated Antibody

For research use only

Citations for Human CCL8/MCP‑2 Biotinylated Antibody

Customer Reviews for Human CCL8/MCP‑2 Biotinylated Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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3 Stars
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1 Stars
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  • Human CCL8/MCP-2 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 12/07/2017
    Good detection in disease samples
  • Human CCL8/MCP-2 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/20/2017
    This antibody was used with MAB281 to build an ELISA to measure MCP-2 in human serum samples.
    Human CCL8/MCP‑2 Biotinylated Antibody BAF281

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