Human CCL8/MCP-2 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF281

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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (2)

Human CCL8/MCP-2 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL8/MCP-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) HCC‑1, recombinant mouse (rm) MIP-1 alpha, rhMCP-1, and rhMCP-3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CCL8/MCP-2 (R&D Systems, Catalog # 281-CP)
Gln24-Pro99
Accession # P80075
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CCL8/MCP‑2 (Catalog # 281-CP)

Human CCL8/MCP-2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human CCL8/MCP‑2 Antibody (Catalog # MAB281)

Standard: Recombinant Human CCL8/MCP-2 Protein (Catalog # 281-CP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL8/MCP-2

MCP-2 and MCP-3 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. MCP-3 also shares 58% amino acid identity with MCP-2.

Similar to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Entrez Gene IDs
6355 (Human); 20307 (Mouse)
Alternate Names
C-C motif chemokine 8; CCL8; chemokine (C-C motif) ligand 8; HC14; MCP2; MCP-2; member 8 (monocyte chemotactic protein 2); monocyte chemoattractant protein 2; SCYA10; small-inducible cytokine A8

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Citations for Human CCL8/MCP-2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. A Toll-like receptor-1 variant and its characteristic cellular phenotype is associated with severe malaria in Papua New Guinean children.
    Authors: Manning L, Cutts J, Stanisic D, Laman M, Carmagnac A, Allen S, O'Donnell A, Karunajeewa H, Rosanas-Urgell A, Siba P, Davis T, Michon P, Schofield L, Rockett K, Kwiatkowski D, Mueller I
    Genes Immun, 2015-12-03;17(1):52-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  2. Allergen challenge in vivo alters rhinovirus-induced chemokine secretion from human airway macrophages.
    Authors: Karta, Maya R, Wickert, Lisa E, Curran, Colleen, Gavala, Monica L, Denlinger, Loren C, Gern, James E, Bertics, Paul J
    J Allergy Clin Immunol, 2014-02-14;133(4):1227-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Development (Detection)
  3. Synergistic up-regulation of MCP-2/CCL8 activity is counteracted by chemokine cleavage, limiting its inflammatory and anti-tumoral effects.
    Authors: Struyf S, Proost P, Vandercappellen J
    Eur. J. Immunol., 2009-03-01;39(3):843-57.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  4. Effect of cognate human CD4+ T cell and endothelial cell interactions upon chemokine production.
    Authors: Tay SS, McCormack A, Rose ML
    Transplantation, 2004-10-15;78(7):987-94.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

FAQs

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Reviews for Human CCL8/MCP-2 Biotinylated Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human CCL8/MCP-2 Biotinylated Antibody
By Anonymous on 12/07/2017
Application: ELISA Sample Tested: Serum Species: Human

Good detection in disease samples


Human CCL8/MCP-2 Biotinylated Antibody
By Anonymous on 11/20/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

This antibody was used with MAB281 to build an ELISA to measure MCP-2 in human serum samples.