Human CCR2 PerCP-conjugated Antibody

Clone 48607 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB151C-025
FAB151C-100
Detection of CCR2 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (4)
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Human CCR2 PerCP-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCR-2 transfectants but not the parental cell line or CCR-5 transfectants.
Source
Monoclonal Mouse IgG2B Clone # 48607
Purification
Protein A or G purified from ascites
Immunogen
NS0 mouse myeloma cell line transfected with human CCR2
Met1-Leu360
Accession # NP_001116868
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR2 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CCR2 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCR2 PerCP-conjugated Monoclonal Antibody (Catalog # FAB151C) or (B) Mouse IgG2BPerCP Isotype Control (Catalog # IC0041C). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR2

CCR2 is a G-protein linked seven transmembrane domain spanning chemokine receptor that preferentially binds monocyte chemoattractant proteins-1 and -3 (MCP-1 and MCP-3). Two isoforms of this receptor (CCR2A and CCR2B) are expressed on cell surfaces as a result of alternate splicing from the same gene. These two CCR2 variants differ only at their intracellular carboxyl terminals, with the CCR2A form possessing 14 additional amino acids. This may provide a mechanism by which cells responding to similar extracellular ligands can activate different intracellular second messengers. Cells that respond to the action of MCP-1 and therefore are likely to express CCR2 receptors, include monocytes, T cells, NK cells, basophils, mast cells and dendritic cells. A recent report suggests that B cells may also express CCR2 receptors. The recognition that a variety of chemokine receptors, including CCR2, can serve as HIV fusion co-factors and as facilitators of T cell recruitment during inflammation makes chemokine receptor monitoring an important exercise in elucidating the HIV infection process and the regulation of inflammatory reactions.

Entrez Gene IDs
729230 (Human); 12772 (Mouse); 60463 (Rat)
Alternate Names
C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2; CC-CKR-2CKR2B; CCR2; CCR-2; CCR2A; CCR2B; CD192 antigen; CD192; chemokine (C-C motif) receptor 2; CKR2; CKR2A; CMKBR2; CMKBR2MGC111760; FLJ78302; MCP-1 receptor; MCP-1-R; MCP-1-RMGC103828; MGC168006; Monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor

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Citations for Human CCR2 PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Chronic heavy drinking drives distinct transcriptional and epigenetic changes in splenic macrophages
    Authors: S Sureshchan, C Stull, BJK Ligh, SB Nguyen, KA Grant, I Messaoudi
    EBioMedicine, 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Tie2 Expressing Monocytes in the Spleen of Patients with Primary Myelofibrosis
    Authors: Rita Campanelli
    PLoS ONE, 2016;11(6):e0156990.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. CX3CL1/CX3CR1 and CCL2/CCR2 Chemokine/Chemokine Receptor Complex in Patients with AMD.
    Authors: Falk M, Singh A, Faber C, Nissen M, Hviid T, Sorensen T
    PLoS ONE, 2014;9(12):e112473.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Secondary lymphoid organ homing phenotype of human myeloid dendritic cells disrupted by an intracellular oral pathogen.
    Authors: Miles, Brodie, Zakhary, Ibrahim, El-Awady, Ahmed, Scisci, Elizabet, Carrion, Julio, O'Neill, John C, Rawlings, Aaron, Stern, J Kobi, Susin, Cristian, Cutler, Christop
    Infect Immun, 2014;82(1):101-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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