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Human CCR2 PE-conjugated Antibody

FAB151P 32 Citations 1 Review Datasheet / COA / SDS
Clone 48607 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB151P-100
FAB151P-025
Detection of CCR2 in Human PBMCs by Flow Cytometry.
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Citations (32)
FAQs
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCR2 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCR-2 transfectants but not the parental cell line or CCR-5 transfectants.
Source
Monoclonal Mouse IgG2B Clone # 48607
Purification
Protein A or G purified from ascites
Immunogen
NS0 mouse myeloma cell line transfected with human CCR2
Met1-Leu360
Accession # NP_001116868
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CCR2 in Human PBMCs by Flow Cytometry. View Larger

Detection of CCR2 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCR2 PE‑conjugated Monoclonal Antibody (Catalog # FAB151P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR2

CCR2 is a G-protein linked seven transmembrane domain spanning chemokine receptor that preferentially binds monocyte chemoattractant proteins-1 and -3 (MCP-1 and MCP-3). Two isoforms of this receptor (CCR2A and CCR2B) are expressed on cell surfaces as a result of alternate splicing from the same gene. These two CCR2 variants differ only at their intracellular carboxyl terminals, with the CCR2A form possessing 14 additional amino acids. This may provide a mechanism by which cells responding to similar extracellular ligands can activate different intracellular second messengers. Cells that respond to the action of MCP-1 and therefore are likely to express CCR2 receptors, include monocytes, T cells, NK cells, basophils, mast cells and dendritic cells. A recent report suggests that B cells may also express CCR2 receptors. The recognition that a variety of chemokine receptors, including CCR2, can serve as HIV fusion co-factors and as facilitators of T cell recruitment during inflammation makes chemokine receptor monitoring an important exercise in elucidating the HIV infection process and the regulation of inflammatory reactions.

Entrez Gene IDs
729230 (Human); 12772 (Mouse); 60463 (Rat)
Alternate Names
C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2CKR2B; CCR2; CCR-2; CCR2A; CCR2B; CD192 antigen; CD192; chemokine (C-C motif) receptor 2; CKR2; CKR2A; CMKBR2MGC111760; FLJ78302; MCP-1 receptor; MCP-1-RMGC103828; MGC168006; Monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor

Product Datasheets

Product Datasheet
COA
SDS

Citations for Human CCR2 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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  1. Role of CXCL13 and CCL20 in the recruitment of B cells to inflammatory foci in chronic arthritis
    Authors: E Armas-Gonz, MJ Domínguez-, A Díaz-Martí, M Arce-Franc, J Castro-Her, G Danelon, V Hernández-, S Bustabad-R, A Cantabrana, M Uguccioni, F Díaz-Gonzá
    Arthritis Res. Ther., 2018;20(1):114.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Extensive Characterization and Comparison of Endothelial Cells Derived from Dermis and Adipose Tissue: Potential Use in Tissue Engineering
    PLoS ONE, 2016;11(11):e0167056.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Phenotypic changes of peripheral blood mononuclear cells upon corticosteroid treatment in idiopathic intermediate uveitis
    Authors: Karoline Walscheid
    Clin. Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Downregulation of Blood Monocyte HLA-DR in ICU Patients Is Also Present in Bone Marrow Cells
    PLoS ONE, 2016;11(11):e0164489.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Buprenorphine decreases the CCL2-mediated chemotactic response of monocytes.
    Authors: Carvallo L, Lopez L, Che F, Lim J, Eugenin E, Williams D, Nieves E, Calderon T, Madrid-Aliste C, Fiser A, Weiss L, Angeletti R, Berman J
    J Immunol, 2015;194(7):3246-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Specificity for a CCR5 Inhibitor Is Conferred by a Single Amino Acid Residue: ROLE OF ILE198.
    Authors: Lau G, Labrecque J, Metz M, Vaz R, Fricker S
    J Biol Chem, 2015;290(17):11041-51.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Cross-desensitization of CCR1, but not CCR2, following activation of the formyl peptide receptor FPR1.
    Authors: Bednar F, Song C, Bardi G, Cornwell W, Rogers T
    J Immunol, 2014;192(11):5305-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
    Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
    J Immunol, 2014;192(6):2634-42.
  9. CX3CL1/CX3CR1 and CCL2/CCR2 Chemokine/Chemokine Receptor Complex in Patients with AMD.
    Authors: Falk M, Singh A, Faber C, Nissen M, Hviid T, Sorensen T
    PLoS ONE, 2014;9(12):e112473.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Adenovirus-engineered human dendritic cells induce natural killer cell chemotaxis via CXCL8/IL-8 and CXCL10/IP-10.
    Authors: Vujanovic, Lazar, Ballard, Wenners, Thorne, Stephen, Vujanovic, Nikola L, Butterfield, Lisa H
    Oncoimmunology, 2012;1(4):448-457.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis.
    Authors: Badr G, Lefevre EA, Mohany M
    PLoS ONE, 2011;6(9):e23741.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
    Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
    J. Immunol., 2010;184(6):2886-98.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Human beta-defensin 2 and 3 and their mouse orthologs induce chemotaxis through interaction with CCR2.
    Authors: Rohrl J, Yang D, Oppenheim JJ, Hehlgans T
    J. Immunol., 2010;184(12):6688-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. CD56highCD16-CD62L- NK cells accumulate in allergic contact dermatitis and contribute to the expression of allergic responses.
    Authors: Carbone T, Nasorri F, Pennino D
    J. Immunol., 2010;184(2):1102-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Impact of heterogeneity of human peripheral blood monocyte subsets on myocardial salvage in patients with primary acute myocardial infarction.
    Authors: Tsujioka H, Imanishi T, Ikejima H, Kuroi A, Takarada S, Tanimoto T, Kitabata H, Okochi K, Arita Y, Ishibashi K, Komukai K, Kataiwa H, Nakamura N, Hirata K, Tanaka A, Akasaka T
    J. Am. Coll. Cardiol., 2009;54(2):130-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. The Lupane-type Triterpene 30-Oxo-calenduladiol Is a CCR5 Antagonist with Anti-HIV-1 and Anti-chemotactic Activities.
    Authors: Barroso-Gonzalez J, El Jaber-Vazdekis N, Garcia-Exposito L, Machado JD, Zarate R, Ravelo AG, Estevez-Braun A, Valenzuela-Fernandez A
    J. Biol. Chem., 2009;284(24):16609-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes.
    Authors: Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F
    J. Leukoc. Biol., 2008;84(4):1109-19.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Expression of CXC and CC type of chemokines and its receptors in tuberculous and non-tuberculous effusions.
    Authors: Pokkali S, Das SD, R L
    Cytokine, 2008;41(3):307-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Chemokine production and chemokine receptor expression by human glioma cells: role of CXCL10 in tumour cell proliferation.
    Authors: Maru SV, Holloway KA, Flynn G, Lancashire CL, Loughlin AJ, Male DK, Romero IA
    J. Neuroimmunol., 2008;199(1):35-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
    Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
    J. Immunol., 2008;181(11):8053-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Nonpathogenic CCR2-tropic SIVrcm after serial passage and its effect on SIVmac infection of Indian rhesus macaques.
    Authors: Ling B, Veazey RS, Marx PA
    Virology, 2008;379(1):38-44.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Bleomycin treatment causes enhancement of virus replication in the lungs of SHIV-infected macaques.
    Authors: Dhillon NK, Pinson D, Dhillon S, Tawfik O, Danley M, Davis M, Nemon O, Mayo M, Kumar A, Tsai YJ, Kumar A, Buch S
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;292(5):L1233-40.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Staphylococcal superantigen-like 5 binds PSGL-1 and inhibits P-selectin-mediated neutrophil rolling.
    Authors: Bestebroer J, Poppelier MJ, Ulfman LH, Lenting PJ, Denis CV, van Kessel KP, van Strijp JA, de Haas CJ
    Blood, 2007;109(7):2936-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. CC chemokine receptor 5 and CXC chemokine receptor 6 expression by lung CD8+ cells correlates with chronic obstructive pulmonary disease severity.
    Authors: Freeman CM, Curtis JL, Chensue SW
    Am. J. Pathol., 2007;171(3):767-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Chemokine receptors in head and neck cancer: association with metastatic spread and regulation during chemotherapy.
    Authors: Muller A, Sonkoly E, Eulert C, Gerber PA, Kubitza R, Schirlau K, Franken-Kunkel P, Poremba C, Snyderman C, Klotz LO, Ruzicka T, Bier H, Zlotnik A, Whiteside TL, Homey B, Hoffmann TK
    Int. J. Cancer, 2006;118(9):2147-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Chemokine receptor expression in EBV-associated lymphoproliferation in hu/SCID mice: implications for CXCL12/CXCR4 axis in lymphoma generation.
    Authors: Piovan E, Tosello V, Indraccolo S, Cabrelle A, Baesso I, Trentin L, Zamarchi R, Tamamura H, Fujii N, Semenzato G, Chieco-Bianchi L, Amadori A
    Blood, 2005;105(3):931-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. CXC-chemokine stimulation of neutrophils correlates with plasma levels of myeloperoxidase and lactoferrin and contributes to clinical outcome after pediatric cardiac surgery.
    Authors: Gessler P, Pretre R, Hohl V, Rousson V, Fischer J, Dahinden C
    Shock, 2004;22(6):513-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. Recapitulation of B cell differentiation in the central nervous system of patients with multiple sclerosis.
    Authors: Casazza S, Zappia E, Pistorio A, Gambini C
    Proc. Natl. Acad. Sci. U.S.A., 2004;101(30):11064-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  29. Chemokine receptor expression on human eosinophils from peripheral blood and bronchoalveolar lavage fluid after segmental antigen challenge.
    Authors: Liu LY, Jarjour NN, Busse WW, Kelly EA
    J. Allergy Clin. Immunol., 2003;112(3):556-62.
    Species: Human
    Sample Types: BALF
    Applications: Flow Cytometry
  30. Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
    Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
    J. Leukoc. Biol., 2003;73(5):584-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  31. Sequential involvement of CCR2 and CCR6 ligands for immature dendritic cell recruitment: possible role at inflamed epithelial surfaces.
    Authors: Vanbervliet B, Homey B, Durand I, Massacrier C, Ait-Yahia S, de Bouteiller O, Vicari A, Caux C
    Eur J Immunol, 2002;32(1):231-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  32. Enhanced tumor trafficking of GD2 chimeric antigen receptor T cells by expression of the chemokine receptor CCR2b.
    Authors: Craddock J, Lu A, Bear A, Pule M, Brenner M, Rooney C, Foster A
    J Immunother, 0;33(8):780-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: CAR-T (Flow Cytometry)

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Mouse IgG2B PE-conjugated Antibody

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Staining Reagents

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Flow Cytometry Staining Buffer (1X)

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Human CCR2 PE-conjugated Antibody
By Anonymous on 01/11/2017
Application: Flow Sample Tested: Whole blood monocytes Species: Human

100ul of freshly drawn whole blood from healthy control subject was stained with anti-human CCR2-PE antibody (FAB151P). Briefly, antibody volume required to stain 10e6 cells was added to 100ul whole blood, mixed well by pipetting and incubated at RT in the dark for 20 minutes. Following that, 1ml of ACK lysis buffer was added to the tube to lyse the RBCs and incubated for 10 minutes. 2ml of FACS staining buffer was added and tube was spun for 10 min at 1200rpm to wash cells. Supernatant was discarded and cells were washed again with 1ml of FACS buffer. Supernatant was discarded after second wash and cells were suspended in 2% Paraformaldehyde until acquisition. Shown in figure are a) FMO serving as negative control showing unstained monocytes and b) anti-human CCR2-PE (FAB151P) stained monocytes from a healthy control.

Flow Cytometry Human CCR2 PE-conjugated Antibody FAB151P