Human CCR2 PE-conjugated Antibody

Clone 48607 was used by HLDA to establish CD designation.
(29 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human CCR-2 transfectants but not the parental cell line or CCR-5 transfectants.
  • Source
    Monoclonal Mouse IgG2B Clone # 48607
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    NS0 mouse myeloma cell line transfected with human CCR2
    Met1-Leu360
    Accession # NP_001116868
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR2 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCR2 PE‑conjugated Monoclonal Antibody (Catalog # FAB151P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR2
CCR2 is a G-protein linked seven transmembrane domain spanning chemokine receptor that preferentially binds monocyte chemoattractant proteins-1 and -3 (MCP-1 and MCP-3). Two isoforms of this receptor (CCR2A and CCR2B) are expressed on cell surfaces as a result of alternate splicing from the same gene. These two CCR2 variants differ only at their intracellular carboxyl terminals, with the CCR2A form possessing 14 additional amino acids. This may provide a mechanism by which cells responding to similar extracellular ligands can activate different intracellular second messengers. Cells that respond to the action of MCP-1 and therefore are likely to express CCR2 receptors, include monocytes, T cells, NK cells, basophils, mast cells and dendritic cells. A recent report suggests that B cells may also express CCR2 receptors. The recognition that a variety of chemokine receptors, including CCR2, can serve as HIV fusion co-factors and as facilitators of T cell recruitment during inflammation makes chemokine receptor monitoring an important exercise in elucidating the HIV infection process and the regulation of inflammatory reactions.
  • Entrez Gene IDs:
    729230 (Human); 12772 (Mouse); 60463 (Rat)
  • Alternate Names:
    C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2CKR2B; CCR2; CCR-2; CCR2A; CCR2B; CD192 antigen; CD192; chemokine (C-C motif) receptor 2; CKR2; CKR2A; CMKBR2MGC111760; FLJ78302; MCP-1 receptor; MCP-1-RMGC103828; MGC168006; Monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Downregulation of Blood Monocyte HLA-DR in ICU Patients Is Also Present in Bone Marrow Cells
    PLoS ONE, 2016;11(11):e0164489.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Extensive Characterization and Comparison of Endothelial Cells Derived from Dermis and Adipose Tissue: Potential Use in Tissue Engineering
    PLoS ONE, 2016;11(11):e0167056.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Buprenorphine decreases the CCL2-mediated chemotactic response of monocytes.
    Authors: Carvallo L, Lopez L, Che F, Lim J, Eugenin E, Williams D, Nieves E, Calderon T, Madrid-Aliste C, Fiser A, Weiss L, Angeletti R, Berman J
    J Immunol, 2015;194(7):3246-58.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Specificity for a CCR5 Inhibitor Is Conferred by a Single Amino Acid Residue: ROLE OF ILE198.
    Authors: Lau G, Labrecque J, Metz M, Vaz R, Fricker S
    J Biol Chem, 2015;290(17):11041-51.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
    Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
    J Immunol, 2014;192(6):2634-42.
  6. Cross-desensitization of CCR1, but not CCR2, following activation of the formyl peptide receptor FPR1.
    Authors: Bednar F, Song C, Bardi G, Cornwell W, Rogers T
    J Immunol, 2014;192(11):5305-13.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. CX3CL1/CX3CR1 and CCL2/CCR2 Chemokine/Chemokine Receptor Complex in Patients with AMD.
    Authors: Falk M, Singh A, Faber C, Nissen M, Hviid T, Sorensen T
    PLoS ONE, 2014;9(12):e112473.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Adenovirus-engineered human dendritic cells induce natural killer cell chemotaxis via CXCL8/IL-8 and CXCL10/IP-10.
    Authors: Vujanovic, Lazar, Ballard, Wenners, Thorne, Stephen, Vujanovic, Nikola L, Butterfield, Lisa H
    Oncoimmunology, 2012;1(4):448-457.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis.
    Authors: Badr G, Lefevre EA, Mohany M
    PLoS ONE, 2011;6(9):e23741.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
    Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
    J. Immunol., 2010;184(6):2886-98.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. CD56highCD16-CD62L- NK cells accumulate in allergic contact dermatitis and contribute to the expression of allergic responses.
    Authors: Carbone T, Nasorri F, Pennino D
    J. Immunol., 2010;184(2):1102-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  12. Human beta-defensin 2 and 3 and their mouse orthologs induce chemotaxis through interaction with CCR2.
    Authors: Rohrl J, Yang D, Oppenheim JJ, Hehlgans T
    J. Immunol., 2010;184(12):6688-94.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  13. Impact of heterogeneity of human peripheral blood monocyte subsets on myocardial salvage in patients with primary acute myocardial infarction.
    Authors: Tsujioka H, Imanishi T, Ikejima H, Kuroi A, Takarada S, Tanimoto T, Kitabata H, Okochi K, Arita Y, Ishibashi K, Komukai K, Kataiwa H, Nakamura N, Hirata K, Tanaka A, Akasaka T
    J. Am. Coll. Cardiol., 2009;54(2):130-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  14. The Lupane-type Triterpene 30-Oxo-calenduladiol Is a CCR5 Antagonist with Anti-HIV-1 and Anti-chemotactic Activities.
    Authors: Barroso-Gonzalez J, El Jaber-Vazdekis N, Garcia-Exposito L, Machado JD, Zarate R, Ravelo AG, Estevez-Braun A, Valenzuela-Fernandez A
    J. Biol. Chem., 2009;284(24):16609-20.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  15. Expression of CXC and CC type of chemokines and its receptors in tuberculous and non-tuberculous effusions.
    Authors: Pokkali S, Das SD, R L
    Cytokine, 2008;41(3):307-14.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  16. C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes.
    Authors: Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F
    J. Leukoc. Biol., 2008;84(4):1109-19.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  17. Nonpathogenic CCR2-tropic SIVrcm after serial passage and its effect on SIVmac infection of Indian rhesus macaques.
    Authors: Ling B, Veazey RS, Marx PA
    Virology, 2008;379(1):38-44.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Flow
  18. Chemokine production and chemokine receptor expression by human glioma cells: role of CXCL10 in tumour cell proliferation.
    Authors: Maru SV, Holloway KA, Flynn G, Lancashire CL, Loughlin AJ, Male DK, Romero IA
    J. Neuroimmunol., 2008;199(1):35-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  19. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
    Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
    J. Immunol., 2008;181(11):8053-67.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  20. Staphylococcal superantigen-like 5 binds PSGL-1 and inhibits P-selectin-mediated neutrophil rolling.
    Authors: Bestebroer J, Poppelier MJ, Ulfman LH, Lenting PJ, Denis CV, van Kessel KP, van Strijp JA, de Haas CJ
    Blood, 2007;109(7):2936-43.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  21. Bleomycin treatment causes enhancement of virus replication in the lungs of SHIV-infected macaques.
    Authors: Dhillon NK, Pinson D, Dhillon S, Tawfik O, Danley M, Davis M, Nemon O, Mayo M, Kumar A, Tsai YJ, Kumar A, Buch S
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;292(5):L1233-40.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Flow
  22. CC chemokine receptor 5 and CXC chemokine receptor 6 expression by lung CD8+ cells correlates with chronic obstructive pulmonary disease severity.
    Authors: Freeman CM, Curtis JL, Chensue SW
    Am. J. Pathol., 2007;171(3):767-76.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  23. Chemokine receptors in head and neck cancer: association with metastatic spread and regulation during chemotherapy.
    Authors: Muller A, Sonkoly E, Eulert C, Gerber PA, Kubitza R, Schirlau K, Franken-Kunkel P, Poremba C, Snyderman C, Klotz LO, Ruzicka T, Bier H, Zlotnik A, Whiteside TL, Homey B, Hoffmann TK
    Int. J. Cancer, 2006;118(9):2147-57.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  24. Chemokine receptor expression in EBV-associated lymphoproliferation in hu/SCID mice: implications for CXCL12/CXCR4 axis in lymphoma generation.
    Authors: Piovan E, Tosello V, Indraccolo S, Cabrelle A, Baesso I, Trentin L, Zamarchi R, Tamamura H, Fujii N, Semenzato G, Chieco-Bianchi L, Amadori A
    Blood, 2005;105(3):931-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  25. Recapitulation of B cell differentiation in the central nervous system of patients with multiple sclerosis.
    Authors: Casazza S, Zappia E, Pistorio A, Gambini C
    Proc. Natl. Acad. Sci. U.S.A., 2004;101(30):11064-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  26. CXC-chemokine stimulation of neutrophils correlates with plasma levels of myeloperoxidase and lactoferrin and contributes to clinical outcome after pediatric cardiac surgery.
    Authors: Gessler P, Pretre R, Hohl V, Rousson V, Fischer J, Dahinden C
    Shock, 2004;22(6):513-20.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  27. Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
    Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
    J. Leukoc. Biol., 2003;73(5):584-90.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  28. Chemokine receptor expression on human eosinophils from peripheral blood and bronchoalveolar lavage fluid after segmental antigen challenge.
    Authors: Liu LY, Jarjour NN, Busse WW, Kelly EA
    J. Allergy Clin. Immunol., 2003;112(3):556-62.
    Species: Human
    Sample Type: BALF
    Application: Flow
  29. Sequential involvement of CCR2 and CCR6 ligands for immature dendritic cell recruitment: possible role at inflamed epithelial surfaces.
    Authors: Vanbervliet B, Homey B, Durand I, Massacrier C, Ait-Yahia S, de Bouteiller O, Vicari A, Caux C
    Eur J Immunol, 2002;32(1):231-42.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 29 Citations
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Mouse IgG2B PE-conjugated Antibody

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Flow Cytometry Human Lyse Buffer (10X)

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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Human CCR2 PE-conjugated Antibody FAB151P
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 Flow Human Anonymous 01/11/2017
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Flow Cytometry Human CCR2 PE-conjugated Antibody FAB151P
Flow Cytometry: Human CCR2 PE-conjugated Antibody [FAB151P]

Summary

ApplicationFlow Cytometry
Sample TestedWhole blood monocytes
SpeciesHuman

Other Experimental Details

Other Experimental Details100ul of freshly drawn whole blood from healthy control subject was stained with anti-human CCR2-PE antibody (FAB151P). Briefly, antibody volume required to stain 10e6 cells was added to 100ul whole blood, mixed well by pipetting and incubated at RT in the dark for 20 minutes. Following that, 1ml of ACK lysis buffer was added to the tube to lyse the RBCs and incubated for 10 minutes. 2ml of FACS staining buffer was added and tube was spun for 10 min at 1200rpm to wash cells. Supernatant was discarded and cells were washed again with 1ml of FACS buffer. Supernatant was discarded after second wash and cells were suspended in 2% Paraformaldehyde until acquisition. Shown in figure are a) FMO serving as negative control showing unstained monocytes and b) anti-human CCR2-PE (FAB151P) stained monocytes from a healthy control.

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