Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, CAR-T (Flow Cytometry)

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 48607
View all CCR2 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CCR2
Met1-Leu360
Accession # NP_001116868

Specificity

Detects human CCR-2 transfectants but not the parental cell line or CCR-5 transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human CCR2 PE‑conjugated Antibody

Detection of CCR2 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CCR2 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCR2 PE-conjugated Monoclonal Antibody (Catalog # FAB151P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.
Detection of Human CCR2 by Flow Cytometry

Detection of Human CCR2 by Flow Cytometry

Monocyte differentiation stages in the BM.Monocyte differentiation stages were characterized by CD45, CD34, CD33 and CD14 expression. HLA-DR and CCR2 expression were measured in each stage and expressed as the number of sites per cell. a/ example of FACs analysis for monocyte differentiation (red: myelo/monoblasts, blue: promonocytes, green: monocytes), and the HLA-DR and CCR2 expression levels in each stage (patient 31, see S1 Table); b/ individual values for HLA-DR expression (n = 10); c/ individual values for CCR2 expression (n = 10 for myelo/monoblasts, n = 11 for other cell populations). Wilcoxon comparisons. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0164489), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CCR2 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Reviewed Applications

Read 6 reviews rated 4.3 using FAB151P in the following applications:

Spectra Viewer

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR2

CCR2 is a G-protein linked seven transmembrane domain spanning chemokine receptor that preferentially binds monocyte chemoattractant proteins-1 and -3 (MCP-1 and MCP-3). Two isoforms of this receptor (CCR2A and CCR2B) are expressed on cell surfaces as a result of alternate splicing from the same gene. These two CCR2 variants differ only at their intracellular carboxyl terminals, with the CCR2A form possessing 14 additional amino acids. This may provide a mechanism by which cells responding to similar extracellular ligands can activate different intracellular second messengers. Cells that respond to the action of MCP-1 and therefore are likely to express CCR2 receptors, include monocytes, T cells, NK cells, basophils, mast cells and dendritic cells. A recent report suggests that B cells may also express CCR2 receptors. The recognition that a variety of chemokine receptors, including CCR2, can serve as HIV fusion co-factors and as facilitators of T cell recruitment during inflammation makes chemokine receptor monitoring an important exercise in elucidating the HIV infection process and the regulation of inflammatory reactions.

Alternate Names

CC-CKR-2, CCR2, CCR2B, CD192, CKR2, CKR2A, CMKBR2, FLJ78302, MCP-1-R

Entrez Gene IDs

729230 (Human); 12772 (Mouse); 60463 (Rat)

Gene Symbol

CCR2

UniProt

NP_001116868

Additional CCR2 Products

Product Documents for Human CCR2 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCR2 PE‑conjugated Antibody

For research use only

Citations for Human CCR2 PE‑conjugated Antibody

Customer Reviews for Human CCR2 PE‑conjugated Antibody (6)

4.3 out of 5
6 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 of 6 reviews Showing All
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  • Human CCR2 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 01/24/2021
    Human CCR2 PE‑conjugated Antibody FAB151P
  • Human CCR2 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Whole blood monocytes
    Species: Human
    Verified Customer | Posted 01/11/2017
    100ul of freshly drawn whole blood from healthy control subject was stained with anti-human CCR2-PE antibody (FAB151P). Briefly, antibody volume required to stain 10e6 cells was added to 100ul whole blood, mixed well by pipetting and incubated at RT in the dark for 20 minutes. Following that, 1ml of ACK lysis buffer was added to the tube to lyse the RBCs and incubated for 10 minutes. 2ml of FACS staining buffer was added and tube was spun for 10 min at 1200rpm to wash cells. Supernatant was discarded and cells were washed again with 1ml of FACS buffer. Supernatant was discarded after second wash and cells were suspended in 2% Paraformaldehyde until acquisition. Shown in figure are a) FMO serving as negative control showing unstained monocytes and b) anti-human CCR2-PE (FAB151P) stained monocytes from a healthy control.
    Human CCR2 PE‑conjugated Antibody FAB151P
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 24005868
    Species: Human
    Verified Customer | Posted 01/21/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 24005771
    Species: Human
    Verified Customer | Posted 01/21/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 24005868
    Species: Human
    Verified Customer | Posted 01/21/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 24005771
    Species: Human
    Verified Customer | Posted 01/21/2015

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