Human CCR2 Antibody

Clone 48607 was used by HLDA to establish CD designation.
(29 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CCR-2 transfectants but not the parental cell line or CCR-5 transfectants.
  • Source
    Monoclonal Mouse IgG2B Clone # 48607
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    NS0 mouse myeloma cell line transfected with human CCR2
    Met1-Leu360
    Accession # NP_001116868
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR2 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human CCR2 Monoclonal Antibody (Catalog # MAB150) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Immunohistochemistry
CCR2 in Human Tonsil. CCR2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CCR2 Monoclonal Antibody (Catalog # MAB150) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCR2
CCR2 is a G-protein linked seven transmembrane domain spanning chemokine receptor that preferentially binds monocyte chemoattractant proteins-1 and -3 (MCP-1 and MCP-3). Two isoforms of this receptor (CCR2A and CCR2B) are expressed on cell surfaces as a result of alternate splicing from the same gene. These two CCR2 variants differ only at their intracellular carboxyl terminals, with the CCR2A form possessing 14 additional amino acids. This may provide a mechanism by which cells responding to similar extracellular ligands can activate different intracellular second messengers. Cells that respond to the action of MCP-1 and therefore are likely to express CCR2 receptors, include monocytes, T cells, NK cells, basophils, mast cells and dendritic cells. A recent report suggests that B cells may also express CCR2 receptors. The recognition that a variety of chemokine receptors, including CCR2, can serve as HIV fusion co-factors and as facilitators of T cell recruitment during inflammation makes chemokine receptor monitoring an important exercise in elucidating the HIV infection process and the regulation of inflammatory reactions.
  • Entrez Gene IDs:
    729230 (Human); 12772 (Mouse); 60463 (Rat)
  • Alternate Names:
    C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2CKR2B; CCR2; CCR-2; CCR2A; CCR2B; CD192 antigen; CD192; chemokine (C-C motif) receptor 2; CKR2; CKR2A; CMKBR2MGC111760; FLJ78302; MCP-1 receptor; MCP-1-RMGC103828; MGC168006; Monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Macrophage infiltration and genetic landscape of undifferentiated uterine sarcomas
    Authors: J Przybyl, M Kowalewska, A Quattrone, B Dewaele, V Vanspauwen, S Varma, S Vennam, AM Newman, M Swierniak, E Baku?a-Zal, JA Siedlecki, M Bidzinski, J Cools, M van de Rij, M Debiec-Ryc
    JCI Insight, 2017;2(11):.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  2. Regulatory T-cells from pancreatic lymphnodes of patients with type-1 diabetes express increased levels of microRNA miR-125a-5p that limits CCR2 expression
    Authors: G Sebastiani, G Ventriglia, A Stabilini, C Socci, C Morsiani, A Laurenzi, L Nigi, C Formichi, B Mfarrej, A Petrelli, G Fousteri, TM Brusko, F Dotta, M Battaglia
    Sci Rep, 2017;7(1):6897.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. CSF-1 regulates the function of monocytes in Crohn's disease patients in remission
    Authors: JC Nieto, C Zamora, E Cantó, E Garcia-Pla, J Gordillo, MA Ortiz, C Juárez, S Vidal
    Sci Rep, 2017;7(1):92.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Evidence for chemokine synergy during neutrophil migration in ARDS
    Thorax, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. JAM-A and ALCAM are therapeutic targets to inhibit diapedesis across the BBB of CD14+CD16+ monocytes in HIV-infected individuals.
    Authors: Williams D, Anastos K, Morgello S, Berman J
    J Leukoc Biol, 2015;97(2):401-12.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. HIV-1 Nef down-modulates C-C and C-X-C chemokine receptors via ubiquitin and ubiquitin-independent mechanism.
    Authors: Chandrasekaran, Prabha, Moore, Victoria, Buckley, Monica, Spurrier, Joshua, Kehrl, John H, Venkatesan, Sundarar
    PLoS ONE, 2014;9(1):e86998.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. PC3-secreted microprotein is a novel chemoattractant protein and functions as a high-affinity ligand for CC chemokine receptor 2.
    Authors: Pei X, Sun Q, Zhang Y, Wang P, Peng X, Guo C, Xu E, Zheng Y, Mo X, Ma J, Chen D, Zhang Y, Zhang Y, Song Q, Guo S, Shi T, Zhang Z, Ma D, Wang Y
    J Immunol, 2014;192(4):1878-86.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Derivation of injury-responsive dendritic cells for acute brain targeting and therapeutic protein delivery in the stroke-injured rat.
    Authors: Manley, Nathan C, Caso, Javier R, Works, Melissa, Cutler, Andrew B, Zemlyak, Ilona, Sun, Guohua, Munhoz, Carolina, Chang, Sydney, Sorrells, Shawn F, Ermini, Florian, Decker, Johannes, Bertrand, Anthony, Dinkel, Klaus M, Steinberg, Gary K, Sapolsky, Robert M
    PLoS ONE, 2013;8(4):e61789.
    Species: Rat
    Sample Type: Whole Cells
    Application: Flow
  9. Changes of peripheral TGF-beta1 depend on monocytes-derived macrophages in Huntington disease.
    Authors: Di Pardo A, Alberti S, Maglione V, Amico E, Cortes E, Elifani F, Battaglia G, Busceti C, Nicoletti F, Vonsattel J, Squitieri F
    Mol Brain, 2013;6(0):55.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. Absence of CC chemokine receptors 2a and 2b from human adipose lineage cells.
    Authors: Koenig C, Fischer-Posovszky P, Rojewski M, Tews D, Schrezenmeier H, Wabitsch M, Gierschik P, Moepps B
    Mol Cell Endocrinol, 2013;369(1):72-85.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. Functional diversity of human vaginal APC subsets in directing T-cell responses.
    Authors: Duluc, D, Gannevat, J, Anguiano, E, Zurawski, S, Carley, M, Boreham, M, Stecher, J, Dullaers, M, Banchereau, J, Oh, S
    Mucosal Immunol, 2013;6(3):626-38.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: Flow
  12. Comparison of gene expression profiles between human and mouse monocyte subsets.
    Authors: Ingersoll MA, Spanbroek R, Lottaz C
    Blood, 2010;115(3):e10-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  13. Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7.
    Authors: Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J
    Int. J. Cancer, 2010;127(2):332-44.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  14. Monocyte chemoattractant protein-1 (MCP-1)/CCL2 secreted by hepatic myofibroblasts promotes migration and invasion of human hepatoma cells.
    Authors: Dagouassat M, Suffee N, Hlawaty H, Haddad O, Charni F, Laguillier C, Vassy R, Martin L, Schischmanoff PO, Gattegno L, Oudar O, Sutton A, Charnaux N
    Int. J. Cancer, 2010;126(5):1095-108.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  15. Chemokine-enhanced chemotaxis of lymphangioleiomyomatosis cells with mutations in the tumor suppressor TSC2 gene.
    Authors: Pacheco-Rodriguez G, Kumaki F, Steagall WK, Zhang Y, Ikeda Y, Lin JP, Billings EM, Moss J
    J. Immunol., 2009;182(3):1270-7.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  16. CCR1 expression and signal transduction by murine BMMC results in secretion of TNF-alpha, TGFbeta-1 and IL-6.
    Authors: Fifadara NH, Aye CC, Raghuwanshi SK, Richardson RM, Ono SJ
    Int. Immunol., 2009;21(8):991-1001.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  17. Synergy between coproduced CC and CXC chemokines in monocyte chemotaxis through receptor-mediated events.
    Authors: Gouwy M, Struyf S, Noppen S, Schutyser E, Springael JY, Parmentier M, Proost P, Van Damme J
    Mol. Pharmacol., 2008;74(2):485-95.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  18. Altered levels of CC chemokines during pulmonary CMV predict BOS and mortality post-lung transplantation.
    Authors: Weigt SS, Elashoff RM, Keane MP, Strieter RM, Gomperts BN, Xue YY, Ardehali A, Gregson AL, Kubak B, Fishbein MC, Saggar R, Ross DJ, Lynch JP, Zisman DA, Belperio JA
    Am. J. Transplant., 2008;8(7):1512-22.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  19. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
    Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
    J. Immunol., 2008;181(11):8053-67.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  20. The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
    Authors: Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J
    Stem Cells, 2007;25(7):1737-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  21. The monocyte chemoattractant protein-1/cognate CC chemokine receptor 2 system affects cell motility in cultured human podocytes.
    Authors: Burt D, Salvidio G, Tarabra E, Barutta F, Pinach S, Dentelli P, Camussi G, Perin PC, Gruden G
    Am. J. Pathol., 2007;171(6):1789-99.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  22. HIV-1 tat protein induces a migratory phenotype in human fetal microglia by a CCL2 (MCP-1)-dependent mechanism: possible role in NeuroAIDS.
    Authors: Eugenin EA, Dyer G, Calderon TM, Berman JW
    Glia, 2005;49(4):501-10.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  23. Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses.
    Authors: Lim HW, Hillsamer P, Kim CH
    J. Clin. Invest., 2004;114(11):1640-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  24. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
    Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
    J. Immunol., 2004;173(11):6592-602.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  25. Distinct transcriptional programs activated by interleukin-10 with or without lipopolysaccharide in dendritic cells: induction of the B cell-activating chemokine, CXC chemokine ligand 13.
    Authors: Perrier P, Martinez FO, Locati M, Bianchi G, Nebuloni M, Vago G, Bazzoni F, Sozzani S, Allavena P, Mantovani A
    J. Immunol., 2004;172(11):7031-42.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  26. A CCR2-V64I polymorphism affects stability of CCR2A isoform.
    Authors: Nakayama EE, Tanaka Y, Nagai Y, Iwamoto A, Shioda T
    AIDS, 2004;18(5):729-38.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  27. Natural killer T cells infiltrate neuroblastomas expressing the chemokine CCL2.
    Authors: Metelitsa LS, Wu HW, Wang H, Yang Y, Warsi Z, Asgharzadeh S, Groshen S, Wilson SB, Seeger RC
    J. Exp. Med., 2004;199(9):1213-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  28. Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
    Authors: Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T
    Blood, 2003;103(7):2806-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  29. Epitope mapping of CCR5 reveals multiple conformational states and distinct but overlapping structures involved in chemokine and coreceptor function.
    Authors: Lee B, Sharron M, Blanpain C, Doranz BJ, Vakili J, Setoh P, Berg E, Liu G, Guy HR, Durell SR, Parmentier M, Chang CN, Price K, Tsang M, Doms RW
    J. Biol. Chem., 1999;274(14):9617-26.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 29 Citations
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