Human CCR2 Antibody (48607) [Biotin]

Name: Human CCR2 Antibody (48607) [Biotin]
Brand: R&D Systems
SKU: FAB151B
Price: 369 USD
Availability: InStock

R&D Systems | Catalog # FAB151B

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Citations (12)

Product Documents

Clone 48607 was used by HLDA to establish CD designation

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Neutralizing

Label

Biotin

Antibody Source

Monoclonal Mouse IgG_2B Clone # 48607

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Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CCR2
Met1-Leu360
Accession # NP_001116868

Specificity

Detects human CCR2 transfectants but not the parental cell line or CCR5 transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human CCR2 Antibody (48607) [Biotin]

Detection of CCR2 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3832F) and either (A) Mouse Anti-Human CCR2 Biotinylated Monoclonal Antibody (Catalog # FAB151B) or (B) Mouse IgG_2BBiotinylated Isotype Control (Catalog # IC0041B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CCR2 Antibody (48607) [Biotin]

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR2

CCR2 is a G-protein linked seven transmembrane domain spanning chemokine receptor that preferentially binds Monocyte Chemoattractant Proteins-1 and -3 (MCP-1 and MCP-3). Two isoforms of this receptor (CCR2A and CCR2B) are expressed on cell surfaces as a result of alternate splicing from the same gene. These two CCR2 variants differ only at their intracellular carboxyl terminals, with the CCR2A form possessing 14 additional amino acids. This may provide a mechanism by which cells responding to similar extracellular ligands can activate different intracellular second messengers. Cells that respond to the action of MCP-1 and therefore are likely to express CCR2 receptors, include monocytes, T cells, NK cells, basophils, mast cells and dendritic cells. A recent report suggests that B cells may also express CCR2 receptors. The recognition that a variety of chemokine receptors, including CCR2, can serve as HIV fusion co-factors and as facilitators of T cell recruitment during inflammation makes chemokine receptor monitoring an important exercise in elucidating the HIV infection process and the regulation of inflammatory reactions.