Detects human CCR3 transfectants in flow cytometry. In flow cytometry, no cross-reactivity with CCR1, CCR2, CCR5, and CCR6 transfectants or the parental cell line is observed. Detects CCR3 on peripheral blood eosinophils.
Monoclonal Rat IgG2A Clone # 61828
Protein A or G purified from hybridoma culture supernatant
Y3 rat myeloid cell line transfected with human CCR3 Accession # P51677
Supplied in a saline solution containing BSA and Sodium Azide.
PerCP (Peridinin-chlorophyll Protein Complex)
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CCR3 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Rat Anti-Human CCR3 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB155C) and Mouse Anti-Human Integrin alpha 4/CD49d APC‑conjugated Monoclonal Antibody (Catalog # FAB1354A). Quadrant markers were set based on control antibody staining (Catalog # IC006C). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CCR3 is a G protein-linked seven transmembrane chemokine receptor that preferentially binds eotaxin and eotaxin-2.
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