Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Neutralization, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Neutralization, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 61828
View all CCR3 Primary Antibodies »

Product Specifications

Immunogen

Y3 rat myeloid cell line transfected with human CCR3
Accession # P51677

Specificity

Detects human CCR3 transfectants in flow cytometry. In flow cytometry, no cross-reactivity with CCR1, CCR2, CCR5, and CCR6 transfectants or the parental cell line is observed. Detects CCR3 on peripheral blood eosinophils.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CCR3 Antibody

CCR3 antibody in Human Lymph Node by Immunohistochemistry (IHC-P).

CCR3 in Human Lymph Node.

CCR3 was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Rat Anti-Human CCR3 Monoclonal Antibody (Catalog # MAB155) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS018) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Chemotaxis Induced by CCL11/Eotaxin and Neutralization by Human CCR3 Antibody.

Chemotaxis Induced by CCL11/Eotaxin and Neutralization by Human CCR3 Antibody.

Recombinant Human CCL11/Eotaxin (Catalog # 320-EO) chemoattracts the BaF3 mouse proB cell line transfected with human CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant CCL11/Eotaxin (0.333 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human CCR3 Monoclonal Antibody (Catalog # MAB155). The ND50 is typically 0.05-0.25 µg/mL.

Applications for Human CCR3 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human CD49d+ peripheral blood cells

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymph node

Neutralization

Measured by its ability to neutralize CCL11/Eotaxin-induced chemotaxis in the BaF3 mouse proB cell line transfected with human CCR3. The Neutralization Dose (ND50) is typically 0.05-0.25 µg/mL in the presence of 0.333 µg/mL Recombinant Human CCL11/Eotaxin.

Reviewed Applications

Read 1 review rated 5 using MAB155 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCR3

CCR3 is a G protein-linked seven transmembrane chemokine receptor that preferentially binds eotaxin and eotaxin-2.

Alternate Names

CCR3, CD193

Entrez Gene IDs

1232 (Human); 12771 (Mouse); 117027 (Rat); 448802 (Canine)

Gene Symbol

CCR3

UniProt

P51677

Additional CCR3 Products

Product Documents for Human CCR3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CCR3 Antibody

For research use only

Citations for Human CCR3 Antibody

Customer Reviews for Human CCR3 Antibody (1)

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  • Human CCR3 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Melanoma tissue
    Species: Human
    Verified Customer | Posted 10/01/2021
    Human CCR3 Antibody MAB155

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Protocols

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