Human CCR3 Fluorescein-conjugated Antibody FAB155F-025: R&D Systems

Human CCR3 Fluorescein-conjugated Antibody

Clone 61828 was used by HLDA to establish CD designation.
(8 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CCR3 transfectants in flow cytometry. In flow cytometry, no cross-reactivity with CCR1, CCR2, CCR5, and CCR6 transfectants or the parental cell line is observed. Detects CCR3 on peripheral blood eosinophils.
  • Source
    Monoclonal Rat IgG2A Clone # 61828
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Y3 rat myeloid cell line transfected with human CCR3
    Accession # P51677
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR3 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human Integrin  alpha 4/CD49d APC‑conjugated Monoclonal Antibody (Catalog # FAB1354A) and either (A) Rat Anti-Human CCR3 Fluorescein‑conjugated Monoclonal Antibody
(Catalog # FAB155F) or (B) Rat IgG2A Fluorescein Isotype Control (Catalog # IC006F). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR3
CCR3 is a G protein-linked seven transmembrane chemokine receptor that preferentially binds eotaxin and eotaxin-2.
  • Entrez Gene IDs:
    1232 (Human); 12771 (Mouse); 117027 (Rat); 448802 (Canine)
  • Alternate Names:
    b-chemokine receptor; CC chemokine receptor 3; C-C chemokine receptor type 3; C-C CKR-3; CCR3; CCR-3; CD193 antigen; CD193; chemokine (C-C motif) receptor 3; CKR3CC-CKR-3CMKBR3CD193; eosinophil CC chemokine receptor 3; Eosinophil eotaxin receptor; MGC102841
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Chemokine receptor expression on allergen-specific T cells in asthma and allergic bronchopulmonary aspergillosis.
    Authors: Garcia G, Humbert M, Capel F, Rimaniol AC, Escourrou P, Emilie D, Godot V
    Allergy, 2007;62(2):170-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. CC chemokine receptor 5 and CXC chemokine receptor 6 expression by lung CD8+ cells correlates with chronic obstructive pulmonary disease severity.
    Authors: Freeman CM, Curtis JL, Chensue SW
    Am. J. Pathol., 2007;171(3):767-76.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Modulation of eotaxin-3 (CCL26) in alveolar type II epithelial cells.
    Authors: Abonyo BO, Lebby KD, Tonry JH, Ahmad M, Heiman AS
    Cytokine, 2006;36(5):237-44.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Anti-IL-5 (mepolizumab) therapy for eosinophilic esophagitis.
    Authors: Stein ML, Collins MH, Villanueva JM, Kushner JP, Putnam PE, Buckmeier BK, Filipovich AH, Assa'ad AH, Rothenberg ME
    J. Allergy Clin. Immunol., 2006;118(6):1312-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Modulation of GM-CSF release by enantiomers of beta-agonists in human airway smooth muscle.
    Authors: Ameredes BT, Calhoun WJ
    J. Allergy Clin. Immunol., 2005;116(1):65-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
    Authors: Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
    Immunology, 2004;112(2):274-89.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Regulation of CCR5 expression and MIP-1alpha production in CD4+ T cells from patients with rheumatoid arthritis.
    Authors: Wang CR, Liu MF
    Clin. Exp. Immunol., 2003;132(2):371-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
    Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
    J. Leukoc. Biol., 2003;73(5):584-90.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Fluorescein-conjugated Antibody

Ctrl IC006F 2  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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