Human CCR3 Fluorescein-conjugated Antibody

Clone 61828 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB155F-025
FAB155F-100
Detection of CCR3 in Human Blood Granulocytes by Flow Cytometry.
1 Image
Product Details
Citations (9)
FAQs
Supplemental Products
Reviews

Human CCR3 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCR3 transfectants in flow cytometry. In flow cytometry, no cross-reactivity with CCR1, CCR2, CCR5, and CCR6 transfectants or the parental cell line is observed. Detects CCR3 on peripheral blood eosinophils.
Source
Monoclonal Rat IgG2A Clone # 61828
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Y3 rat myeloid cell line transfected with human CCR3
Accession # P51677
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CCR3 in Human Blood Granulocytes by Flow Cytometry. View Larger

Detection of CCR3 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human Integrin  alpha 4/CD49d APC‑conjugated Monoclonal Antibody (Catalog # FAB1354A) and either (A) Rat Anti-Human CCR3 Fluorescein‑conjugated Monoclonal Antibody
(Catalog # FAB155F) or (B) Rat IgG2A Fluorescein Isotype Control (Catalog # IC006F). View our protocol for Staining Membrane-associated Proteins

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR3

CCR3 is a G protein-linked seven transmembrane chemokine receptor that preferentially binds eotaxin and eotaxin-2.

Entrez Gene IDs
1232 (Human); 12771 (Mouse); 117027 (Rat); 448802 (Canine)
Alternate Names
b-chemokine receptor; CC chemokine receptor 3; C-C chemokine receptor type 3; C-C CKR-3; CCR3; CCR-3; CD193 antigen; CD193; chemokine (C-C motif) receptor 3; CKR3CC-CKR-3CMKBR3CD193; eosinophil CC chemokine receptor 3; Eosinophil eotaxin receptor; MGC102841

Product Datasheets

Citations for Human CCR3 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
Filter your results:

Filter by:

  1. Detection of Primary T Cell Responses to Drugs and Chemicals in HLA-Typed Volunteers: Implications for the Prediction of Drug Immunogenicity
    Authors: Lee Faulkner
    Toxicol. Sci, 2016;154(2):416-429.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  2. Chemokine receptor expression on allergen-specific T cells in asthma and allergic bronchopulmonary aspergillosis.
    Authors: Garcia G, Humbert M, Capel F, Rimaniol AC, Escourrou P, Emilie D, Godot V
    Allergy, 2007;62(2):170-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  3. CC chemokine receptor 5 and CXC chemokine receptor 6 expression by lung CD8+ cells correlates with chronic obstructive pulmonary disease severity.
    Authors: Freeman CM, Curtis JL, Chensue SW
    Am. J. Pathol., 2007;171(3):767-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  4. Modulation of eotaxin-3 (CCL26) in alveolar type II epithelial cells.
    Authors: Abonyo BO, Lebby KD, Tonry JH, Ahmad M, Heiman AS
    Cytokine, 2006;36(5):237-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  5. Anti-IL-5 (mepolizumab) therapy for eosinophilic esophagitis.
    Authors: Stein ML, Collins MH, Villanueva JM, Kushner JP, Putnam PE, Buckmeier BK, Filipovich AH, Assa'ad AH, Rothenberg ME
    J. Allergy Clin. Immunol., 2006;118(6):1312-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  6. Modulation of GM-CSF release by enantiomers of beta-agonists in human airway smooth muscle.
    Authors: Ameredes BT, Calhoun WJ
    J. Allergy Clin. Immunol., 2005;116(1):65-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  7. Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
    Authors: Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
    Immunology, 2004;112(2):274-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  8. Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
    Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
    J. Leukoc. Biol., 2003;73(5):584-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  9. Regulation of CCR5 expression and MIP-1alpha production in CD4+ T cells from patients with rheumatoid arthritis.
    Authors: Wang CR, Liu MF
    Clin. Exp. Immunol., 2003;132(2):371-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human CCR3 Fluorescein-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human CCR3 Fluorescein-conjugated Antibody and earn rewards!

Have you used Human CCR3 Fluorescein-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review