Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 112509
View all CCR9 Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with human CCR9
Met1-Leu369
Accession # P51686

Specificity

Specifically stains human CCR9 transfectants and MOLT-4 cells. It does not react with the parental BaF3 cells or human CCR7-transfected BaF3 cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CCR9 APC‑conjugated Antibody

Detection of CCR9 antibody in MOLT-4 Human Cell Line antibody by Flow Cytometry.

Detection of CCR9 in MOLT-4 Human Cell Line by Flow Cytometry.

MOLT-4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CCR9 APC-conjugated Monoclonal Antibody (Catalog # FAB179A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CCR9 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: MOLT‑4 human acute lymphoblastic leukemia cell line

Reviewed Applications

Read 1 review rated 4 using FAB179A in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR9

CCR9 is a G protein-linked seven transmembrane domain cytokine receptor. CCR9 serves as a receptor for CCL25/TECK. It is expressed on mature and immature thymocytes and some peripheral T and B cells.

Alternate Names

CCR9, CD199, CDw199

Entrez Gene IDs

10803 (Human); 12769 (Mouse); 282832 (Rat)

Gene Symbol

CCR9

UniProt

P51686

Additional CCR9 Products

Product Documents for Human CCR9 APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCR9 APC‑conjugated Antibody

For research use only

Citations for Human CCR9 APC‑conjugated Antibody

Customer Reviews for Human CCR9 APC‑conjugated Antibody (1)

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  • Human CCR9 APC‑conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 10/26/2015
    Cells were stained for 10 minutes in room temperature followed by three washes. <br />Specificity: Specific<br />Sensitivity: Not sensitive<br />Buffer: PBS + 2.5% FBS<br />Dilution: 1/10
    Human CCR9 APC‑conjugated Antibody FAB179A

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