Human CCR9 APC-conjugated Antibody

Clone 112509 was used by HLDA to establish CD designation.
(1 citations)
(1 Review)
  • Species Reactivity
  • Specificity
    Specifically stains human CCR9 transfectants and MOLT-4 cells. It does not react with the parental BaF3 cells or human CCR7-transfected BaF3 cells.
  • Source
    Monoclonal Mouse IgG2A Clone # 112509
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    BaF3 mouse pro-B cell line transfected with human CCR9
    Accession # P51686
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR9 in MOLT-4 Human Cell Line by Flow Cytometry. MOLT‑4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CCR9 APC‑conjugated Monoclonal Antibody (Catalog # FAB179A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR9
CCR9 is a G protein-linked seven transmembrane domain cytokine receptor. CCR9 serves as a receptor for CCL25/TECK. It is expressed on mature and immature thymocytes and some peripheral T and B cells.
  • Entrez Gene IDs:
    10803 (Human); 12769 (Mouse); 282832 (Rat)
  • Alternate Names:
    C-C chemokine receptor type 9; C-C CKR-9; CC-CKR-9; CCR9; CCR-9; CD199; CDw199 antigen; CDw199; chemokine (C-C motif) receptor 9; G protein-coupled receptor 28; GPR28; GPR-9-6G-protein coupled receptor 28
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. Immunization of volunteers with Salmonella enterica serovar Typhi strain Ty21a elicits the oligoclonal expansion of CD8+ T cells with predominant Vbeta repertoires.
    Authors: Salerno-Goncalves R, Wahid R, Sztein MB
    Infect. Immun., 2005;73(6):3521-30.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A APC-conjugated Antibody

Ctrl IC003A 1  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Human CCR9 APC‑conjugated Antibody FAB179A
Very Good
 Flow Human Anonymous 10/26/2015
Image Details
Flow Cytometry Human CCR9 APC‑conjugated Antibody FAB179A
Flow Cytometry: Human CCR9 APC‑conjugated Antibody [FAB179A]


ApplicationFlow Cytometry
Sample TestedPeripheral blood mononuclear cells (PBMCs)

Other Experimental Details

Other Experimental DetailsCells were stained for 10 minutes in room temperature followed by three washes.
Specificity: Specific
Sensitivity: Not sensitive
Buffer: PBS + 2.5% FBS
Dilution: 1/10

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