Human CCR9 Fluorescein-conjugated Antibody

Clone 112509 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB179F-025
FAB179F-100
Detection of CCR9 in MOLT-4 Human Cell Line by Flow Cytometry.
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Product Details
Citations (8)
FAQs
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Human CCR9 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Specifically stains human CCR9 transfectants and MOLT-4 cells. It does not react with the parental BaF3 cells or human CCR7-transfected BaF3 cells.
Source
Monoclonal Mouse IgG2A Clone # 112509
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human CCR9
Met1-Leu369
Accession # P51686
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CCR9 antibody in MOLT-4 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of CCR9 in MOLT-4 Human Cell Line by Flow Cytometry. MOLT-4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CCR9 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB179F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR9

CCR9 is a G protein-linked seven transmembrane domain cytokine receptor. CCR9 serves as a receptor for CCL25/TECK. It is expressed on mature and immature thymocytes and some peripheral T and B cells.

Entrez Gene IDs
10803 (Human); 12769 (Mouse); 282832 (Rat)
Alternate Names
C-C chemokine receptor type 9; C-C CKR-9; CC-CKR-9; CCR9; CCR-9; CD199; CDw199 antigen; CDw199; chemokine (C-C motif) receptor 9; G protein-coupled receptor 28; GPR28; GPR-9-6G-protein coupled receptor 28

Product Datasheets

Citations for Human CCR9 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Natural Killer Cells from Allergic Donors Are Defective in Their Response to CCL18 Chemokine
    Authors: L Amniai, C Ple, M Barrier, P de Nadai, P Marquillie, H Vorng, C Chenivesse, A Tsicopoulo, C Duez
    International Journal of Molecular Sciences, 2021;22(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Mucosal cell populations may contribute to peripheral immune abnormalities in HIV-infected subjects introducing cART with moderate immune-suppression
    Authors: M Basilissi, C Tincati, E Merlini, G Ancona, E Borghi, F Borgo, A Barassi, A d'Arminio, G Marchetti
    PLoS ONE, 2019;14(2):e0212075.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Essential role for CCR6 in certain inflammatory diseases demonstrated using specific antagonist and knockin mice
    Authors: R Robert, C Ang, G Sun, L Juglair, EX Lim, LJ Mason, NL Payne, CC Bernard, CR Mackay
    JCI Insight, 2017;2(15):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity
    Authors: Zaidoon Al-Jaderi
    Front Immunol, 2016;7(0):437.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
    Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
    J Immunol, 2014;192(6):2634-42.
  6. CCL25 mediates migration, invasion and matrix metalloproteinase expression by breast cancer cells in a CCR9-dependent fashion.
    Authors: Johnson-Holiday C, Singh R, Johnson E
    Int. J. Oncol., 2011;38(5):1279-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Identification of human NK17/NK1 cells.
    Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
    PLoS ONE, 2011;6(10):e26780.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
    Authors: Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
    Immunology, 2004;112(2):274-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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