Human CCR9 PE-conjugated Antibody

Clone 112509 was used by HLDA to establish CD designation.
(9 citations)   
  • Species Reactivity
    Human
  • Specificity
    Specifically stains human CCR9 transfectants and MOLT-4 cells. It does not react with the parental BaF3 cells or human CCR7-transfected BaF3 cells.
  • Source
    Monoclonal Mouse IgG2A Clone # 112509
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    BaF3 mouse pro-B cell line transfected with human CCR9
    Met1-Leu369
    Accession # P51686
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR9 in MOLT-4 Human Cell Line by Flow Cytometry. MOLT‑4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CCR9 PE‑conjugated Monoclonal Antibody (Catalog # FAB179P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR9
CCR9 is a G protein-linked seven transmembrane domain cytokine receptor. CCR9 serves as a receptor for CCL25/TECK. It is expressed on mature and immature thymocytes and some peripheral T and B cells.
  • Entrez Gene IDs:
    10803 (Human); 12769 (Mouse); 282832 (Rat)
  • Alternate Names:
    C-C chemokine receptor type 9; C-C CKR-9; CC-CKR-9; CCR9; CCR-9; CD199; CDw199 antigen; CDw199; chemokine (C-C motif) receptor 9; G protein-coupled receptor 28; GPR28; GPR-9-6G-protein coupled receptor 28
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species
Applications
Sample Type
  1. Human Mucosa-Associated Invariant T Cells Accumulate in Colon Adenocarcinomas but Produce Reduced Amounts of IFN-gamma.
    Authors: Sundstrom P, Ahlmanner F, Akeus P, Sundquist M, Alsen S, Yrlid U, Borjesson L, Sjoling A, Gustavsson B, Wong S, Quiding-Jarbrink M
    J Immunol, 2015;195(7):3472-81.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Effector Vgamma9Vdelta2 T cells dominate the human fetal gammadelta T-cell repertoire.
    Authors: Dimova T, Brouwer M, Gosselin F, Tassignon J, Leo O, Donner C, Marchant A, Vermijlen D
    Proc Natl Acad Sci U S A, 2015;112(6):E556-65.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Induction and activation of human Th17 by targeting antigens to dendritic cells via dectin-1.
    Authors: Duluc D, Joo H, Ni L, Yin W, Upchurch K, Li D, Xue Y, Klucar P, Zurawski S, Zurawski G, Oh S
    J Immunol, 2014;192(12):5776-88.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Increased numbers of immature plasma cells in peripheral blood specifically overexpress chemokine receptor CXCR3 and CXCR4 in patients with ulcerative colitis.
    Authors: Hosomi S, Oshitani N, Kamata N, Sogawa M, Okazaki H, Tanigawa T, Yamagami H, Watanabe K, Tominaga K, Watanabe T, Fujiwara Y, Maeda K, Hirakawa K, Arakawa T
    Clin. Exp. Immunol., 2011;163(2):215-24.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Human MAIT cells are xenobiotic-resistant, tissue-targeted, CD161hi IL-17-secreting T cells.
    Authors: Dusseaux M, Martin E, Serriari N, Peguillet I, Premel V, Louis D, Milder M, Le Bourhis L, Soudais C, Treiner E, Lantz O
    Blood, 2011;117(4):1250-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
    Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
    J. Immunol., 2010;184(6):2886-98.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Distinct chemokine triggers and in vivo migratory paths of fluorescein dye-labeled T Lymphocytes in acutely simian immunodeficiency virus SIVmac251-infected and uninfected macaques.
    Authors: Clay CC, Rodrigues DS, Harvey DJ, Leutenegger CM, Esser U
    J. Virol., 2005;79(21):13759-68.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Flow
  8. Chemokine networks and in vivo T-lymphocyte trafficking in nonhuman primates.
    Authors: Clay CC, Rodrigues DS, Brignolo LL, Spinner A, Tarara RP, Plopper CG, Leutenegger CM, Esser U
    J. Immunol. Methods, 2004;293(1):23-42.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Flow
  9. Chemokine receptor expression on human eosinophils from peripheral blood and bronchoalveolar lavage fluid after segmental antigen challenge.
    Authors: Liu LY, Jarjour NN, Busse WW, Kelly EA
    J. Allergy Clin. Immunol., 2003;112(3):556-62.
    Species: Human
    Sample Type: BALF
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A PE-conjugated Antibody

Ctrl IC003P 13  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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