Human CCR9 Antibody

Clone 112509 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB179-100
MAB179-SP
MAB179-500
CCR9 in MOLT‑4 Human Cell Line.
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Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (1)

Human CCR9 Antibody Summary

Species Reactivity
Human
Specificity
Specifically stains human CCR9 transfectants and MOLT-4 cells. It does not react with the parental BaF3 cells or human CCR7-transfected BaF3 cells.
Source
Monoclonal Mouse IgG2A Clone # 112509
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human CCR9
Met1-Leu369
Accession # P51686
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
MOLT‑4 human acute lymphoblastic leukemia cell line
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human thymus
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
15-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry CCR9 antibody in MOLT-4 Human Cell Line by Immunocytochemistry (ICC). View Larger

CCR9 in MOLT‑4 Human Cell Line. CCR9 was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using Mouse Anti-Human CCR9 Monoclonal Antibody (Catalog # MAB179) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR9

CCR9 is a G protein-linked seven transmembrane domain cytokine receptor. CCR9 serves as a receptor for CCL25/TECK. It is expressed on mature and immature thymocytes and some peripheral T and B cells.

Entrez Gene IDs
10803 (Human); 12769 (Mouse); 282832 (Rat)
Alternate Names
C-C chemokine receptor type 9; C-C CKR-9; CC-CKR-9; CCR9; CCR-9; CD199; CDw199 antigen; CDw199; chemokine (C-C motif) receptor 9; G protein-coupled receptor 28; GPR28; GPR-9-6G-protein coupled receptor 28

Product Datasheets

Citations for Human CCR9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Novel peptide screened from a phage display library antagonizes the activity of CC chemokine receptor 9
    Authors: Y Hu, A Ma, S Lin, Y Yang, G Hong
    Oncol Lett, 2017;14(6):6471-6476.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Intracellular allosteric antagonism of the CCR9 receptor
    Nature, 2016;540(7633):462-465.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. CCL25 mediates migration, invasion and matrix metalloproteinase expression by breast cancer cells in a CCR9-dependent fashion.
    Authors: Johnson-Holiday C, Singh R, Johnson E
    Int. J. Oncol., 2011;38(5):1279-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Trafficking, persistence, and activation state of adoptively transferred allogeneic and autologous Simian Immunodeficiency Virus-specific CD8(+) T cell clones during acute and chronic infection of rhesus macaques.
    Authors: Bolton DL, Minang JT, Trivett MT, Song K, Tuscher JJ, Li Y, Piatak M, O'Connor D, Lifson JD, Roederer M, Ohlen C
    J. Immunol., 2010;184(1):303-14.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Distribution, persistence, and efficacy of adoptively transferred central and effector memory-derived autologous Simian Immunodeficiency Virus-specific CD8(+) T cell clones in rhesus macaques during acute infection.
    Authors: Minang JT, Trivett MT, Bolton DL, Trubey CM, Estes JD, Li Y, Smedley J, Pung R, Rosati M, Jalah R, Pavlakis GN, Felber BK, Piatak M, Roederer M, Lifson JD, Ott DE, Ohlen C
    J. Immunol., 2010;184(1):315-26.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients.
    Authors: Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D
    J. Immunol., 2007;179(6):4272-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. The monoclonal antibody CHO-131 identifies a subset of cutaneous lymphocyte-associated antigen T cells enriched in P-selectin-binding cells.
    Authors: Ni Z, Campbell JJ, Niehans G, Walcheck B
    J. Immunol., 2006;177(7):4742-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
    Authors: Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T
    Blood, 2003;103(7):2806-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human CCR9 Antibody

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Human CCR9 Antibody
By Anonymous on 10/26/2015
Application: Flow Sample Tested: Peripheral blood cells Species: Human

Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion. Lineage Cocktail 1 (BD Biosciences) comprises CD3, CD14, CD16, CD19, CD20, and CD56 antibodies.
Specificity: Specific
Sensitivity: Sensitive
Buffer: 2% Human Serum, 0.5 mM EDTA in PBS
Dilution: 1/100