Human CCR9 Antibody (112509)

R&D Systems | Catalog # MAB179

Clone 112509 was used by HLDA to establish CD designation
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Neutralization, Flow Cytometry, Immunocytochemistry, Assay Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 112509
View all CCR9 Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with human CCR9
Met1-Leu369
Accession # P51686

Specificity

Specifically stains human CCR9 transfectants and MOLT-4 cells. It does not react with the parental BaF3 cells or human CCR7-transfected BaF3 cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CCR9 Antibody (112509)

CCR9 antibody in MOLT-4 Human Cell Line by Immunocytochemistry (ICC).

CCR9 in MOLT‑4 Human Cell Line.

CCR9 was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using Mouse Anti-Human CCR9 Monoclonal Antibody (Catalog # MAB179) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of CCR9 in MOLT-4 cells by Flow Cytometry

MOLT-4 cells were stained with Mouse Anti-Human CCR9 Monoclonal Antibody (Catalog # MAB179, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CCR9 Antibody (112509)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: MOLT‑4 human acute lymphoblastic leukemia cell line

Immunocytochemistry

15-25 µg/mL
Sample: Immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human thymus

Reviewed Applications

Read 1 review rated 5 using MAB179 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCR9

CCR9 is a G protein-linked seven transmembrane domain cytokine receptor. CCR9 serves as a receptor for CCL25/TECK. It is expressed on mature and immature thymocytes and some peripheral T and B cells.

Alternate Names

CCR9, CD199, CDw199

Entrez Gene IDs

10803 (Human); 12769 (Mouse); 282832 (Rat)

Gene Symbol

CCR9

UniProt

P51686

Additional CCR9 Products

Product Documents for Human CCR9 Antibody (112509)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCR9 Antibody (112509)

For research use only

Citations for Human CCR9 Antibody (112509)

Customer Reviews for Human CCR9 Antibody (112509) (1)

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  • Human CCR9 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood cells
    Species: Human
    Verified Customer | Posted 10/26/2015
    Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion. Lineage Cocktail 1 (BD Biosciences) comprises CD3, CD14, CD16, CD19, CD20, and CD56 antibodies. <br />Specificity: Specific<br />Sensitivity: Sensitive<br />Buffer: 2% Human Serum, 0.5 mM EDTA in PBS<br />Dilution: 1/100
    Human CCR9 Antibody (112509) MAB179

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Protocols

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