Human CCR9 PerCP-conjugated Antibody FAB179C-025: R&D Systems

Human CCR9 PerCP-conjugated Antibody

Clone 112509 was used by HLDA to establish CD designation.
  
  • Species Reactivity
    Human
  • Specificity
    Specifically stains human CCR9 transfectants and MOLT-4 cells. It does not react with the parental BaF3 cells or human CCR7-transfected BaF3 cells.
  • Source
    Monoclonal Mouse IgG2A Clone # 112509
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    BaF3 mouse pro-B cell line transfected with human CCR9
    Met1-Leu369
    Accession # P51686
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR9 in MOLT‑4 Human Cell Line by Flow Cytometry. MOLT‑4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CCR9 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB179C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR9
CCR9 is a G protein-linked seven transmembrane domain cytokine receptor. CCR9 serves as a receptor for CCL25/TECK. It is expressed on mature and immature thymocytes and some peripheral T and B cells.
  • Entrez Gene IDs:
    10803 (Human); 12769 (Mouse); 282832 (Rat)
  • Alternate Names:
    C-C chemokine receptor type 9; C-C CKR-9; CC-CKR-9; CCR9; CCR-9; CD199; CDw199 antigen; CDw199; chemokine (C-C motif) receptor 9; G protein-coupled receptor 28; GPR28; GPR-9-6G-protein coupled receptor 28
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A PerCP-conjugated Isotype Control

Ctrl IC003C 1
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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