Human CD133 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB11331G-025
FAB11331G-100
Detection  of CD133 in NS0 Mouse Cell line Transfected with Human CD133 by Flow  Cytometry.
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Human CD133 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD133.  Stains human CD133 transfectants but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG2A Clone # 170411
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human CD133
Accession # O43490
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of CD133 in NS0 Mouse Cell line Transfected with Human CD133 by Flow  Cytometry. View Larger

Detection of CD133 in NS0 Mouse Cell line Transfected with Human CD133 by Flow Cytometry. NS0 mouse myeloma cell line transfected with either human CD133 (filled histogram) or irrelevant transfectants (open histogram) was stained with Mouse Anti-Human CD133 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB11331G). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD133

CD133 antigen also known as prominin-1 is a glycoprotein that in humans is encoded by the PROM1 gene. It is a pentaspan transmembrane glycoprotein, with highly restricted expression on plasma membrane protrusions of epithelial and other cell types.  CD133 is expressed in hematopoietic stem cells, endothelial progenitor cells, glioblastoma, neuronal and glial stem cells, as well as adult kidney, mammary glands, trachea, salivary glands, placenta, digestive tract, and testes. CD133 is used as a main marker for hematopoietic stem cells in transplant models. It has also been described as a marker of tumor initiating cells. 

Entrez Gene IDs
8842 (Human); 19126 (Mouse)
Alternate Names
AC133 Stargardt disease 4 (autosomal dominant); AC133; CD133 retinal 2; CD133; PROM1; prominin (mouse)-like 1; Prominin 1; PROML1

Product Datasheets

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