Human CD133 Alexa Fluor® 488-conjugated Antibody Summary
Accession # O43490
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD133 in NS0 Mouse Cell line Transfected with Human CD133 by Flow Cytometry. NS0 mouse myeloma cell line transfected with either human CD133 (filled histogram) or irrelevant transfectants (open histogram) was stained with Mouse Anti-Human CD133 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB11331G). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
CD133 antigen also known as prominin-1 is a glycoprotein that in humans is encoded by the PROM1 gene. It is a pentaspan transmembrane glycoprotein, with highly restricted expression on plasma membrane protrusions of epithelial and other cell types. CD133 is expressed in hematopoietic stem cells, endothelial progenitor cells, glioblastoma, neuronal and glial stem cells, as well as adult kidney, mammary glands, trachea, salivary glands, placenta, digestive tract, and testes. CD133 is used as a main marker for hematopoietic stem cells in transplant models. It has also been described as a marker of tumor initiating cells.
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