Human CD133 APC-conjugated Antibody

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Human CD133 APC-conjugated Antibody in Data
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Product Details
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Human CD133 APC-conjugated Antibody Summary

Species Reactivity
Detects human CD133.  Stains human CD133 transfectants but not irrelevant transfectants in flow cytometry.
Monoclonal Mouse IgG2A Clone # 170411
Protein A or G purified from hybridoma culture supernatant
NS0 mouse myeloma cell line transfected with human CD133
Accession # O43490
Supplied in a saline solution containing BSA and Sodium Azide.
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)


Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD133 in NS0 Mouse Cell line Transfected with Human CD133 by Flow Cytometry. View Larger

Detection of CD133 in NS0 Mouse Cell line Transfected with Human CD133 by Flow Cytometry. NS0 mouse myeloma cell line transfected with either human CD133 (filled histogram) or irrelevant transfectants (open histogram) was stained with Mouse Anti-Human CD133 APC‑conjugated Monoclonal Antibody (Catalog # FAB11331A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD133

CD133 antigen also known as prominin-1 is a glycoprotein that in humans is encoded by the PROM1 gene. It is a pentaspan transmembrane glycoprotein, with highly restricted expression on plasma membrane protrusions of epithelial and other cell types.  CD133 is expressed in hematopoietic stem cells, endothelial progenitor cells, glioblastoma, neuronal and glial stem cells, as well as adult kidney, mammary glands, trachea, salivary glands, placenta, digestive tract, and testes. CD133 is used as a main marker for hematopoietic stem cells in transplant models. It has also been described as a marker of tumor initiating cells. 

Entrez Gene IDs
8842 (Human); 19126 (Mouse)
Alternate Names
AC133 Stargardt disease 4 (autosomal dominant); AC133; CD133 retinal 2; CD133; PROM1; prominin (mouse)-like 1; Prominin 1; PROML1

Product Datasheets

Citation for Human CD133 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The FGF, TGF&beta and WNT axis Modulate Self-renewal of Human SIX2+ Urine Derived Renal Progenitor Cells
    Authors: MS Rahman, W Wruck, LS Spitzhorn, L Nguyen, M Bohndorf, S Martins, F Asar, A Ncube, L Erichsen, N Graffmann, J Adjaye
    Sci Rep, 2020;10(1):739.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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