Human CD133 Antibody (170411)

Detection of CD133 in BG01V Human Cells by Flow Cytometry.

BG01V human embryonic stem cells was stained with Mouse Anti-Human CD133 Monoclonal Antibody (Catalog # MAB11331, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B).

Detection of CD133 in iPS cells by Flow Cytometry.

iPS cells were stained with Mouse Anti-Human CD133 Monoclonal Antibody (Catalog # MAB11331, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Detection of CD133 by Flow Cytometry

MDA-MB-231-derived spheroid cells were resistant to doxorubicin administration, and stem cell markers were dramatically induced. (A) Images of MDA-MB-231 cells and cultured spheroids. (B) Flow cytometry detected stemness marker CD133 expression of MDA-MB-231 cells and BCSCs. (C) CCK8 assay analysis showed anti-proliferation of doxorubicin against MDA-MB-231 cells and MDA-MB-231-derived spheroid cells. (D) t-SNE plot cluster distribution, and protein markers including CD133, CD90, vimentin, Nanog, Sox9, CK18, and CD24 expression of spheroid cells before and after being treated with doxorubicin (blue-to-red scale, red represents high expression). (E) Images of cultured stem cell spheres treated with or without doxorubicin. (F) Protein markers’ mean expression level of BCSCs treated with or without doxorubicin. (** p < 0.01, n = 3 biologically independent samples per group). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34831064), licensed under a CC-BY license. Not internally tested by R&D Systems.