Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Western Blot, Immunocytochemistry, ELISA Capture, ELISA Detection

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 170411
View all CD133 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CD133
Accession # O43490

Specificity

Detects human CD133.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CD133 Antibody

Detection of CD133 antibody in BG01V Human Cells antibody by Flow Cytometry.

Detection of CD133 in BG01V Human Cells by Flow Cytometry.

BG01V human embryonic stem cells was stained with Mouse Anti-Human CD133 Monoclonal Antibody (Catalog # MAB11331, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B).

Detection of CD133 in iPS cells by Flow Cytometry.

iPS cells were stained with Mouse Anti-Human CD133 Monoclonal Antibody (Catalog # MAB11331, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD133 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: BG01V human embryonic stem cells and iPS cells

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD133

CD133 antigen also known as prominin-1 is a glycoprotein that in humans is encoded by the PROM1 gene. It is a pentaspan transmembrane glycoprotein, with highly restricted expression on plasma membrane protrusions of epithelial and other cell types.  CD133 is expressed in hematopoietic stem cells, endothelial progenitor cells, glioblastoma, neuronal and glial stem cells, as well as adult kidney, mammary glands, trachea, salivary glands, placenta, digestive tract, and testes. CD133 is used as a main marker for hematopoietic stem cells in transplant models. It has also been described as a marker of tumor initiating cells. 

Alternate Names

AC133, CD133, PROM1, Prominin 1, PROML1

Entrez Gene IDs

8842 (Human); 19126 (Mouse)

Gene Symbol

PROM1

UniProt

O43490

Additional CD133 Products

Product Documents for Human CD133 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CD133 Antibody

For research use only

Citations for Human CD133 Antibody

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