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Human CD15/Lewis X APC-conjugated Antibody

Catalog #: FAB7368A Datasheet / COA / SDS

Discontinued Product

FAB7368A has been discontinued.
View all CD15/Lewis X products.
Detection of CD15/Lewis X in Human Peripheral Blood Granulocytes by Flow Cytometry.
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Human CD15/Lewis X APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD15/Lewis X in direct ELISAs.
Source
Monoclonal Mouse IgM Clone # ICRF29-2
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Immunogen
Human granulocytes
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD15/Lewis X antibody in Human Peripheral Blood Granulocytes antibody by Flow Cytometry. View Larger

Detection of CD15/Lewis X in Human Peripheral Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human CD15/Lewis X APC-conjugated Monoclonal Antibody (Catalog # FAB7368A, filled histogram) or isotype control antibody (Catalog # IC015A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD15/Lewis X

Lewis X (Lex) is a glycan structure (Gal beta 1-4(Fuc alpha 1-3)GlcNAc beta -) that forms the terminus of N-linked carbohydrates such as the antigenic epitope Stage-Specific Embryonic Antigen-1 (SSEA-1/CD15). It is widely expressed, can be found on glycoproteins, glycolipids and proteoglycans and is a blood group antigen. Lex is a marker for mouse embryonic stem and cancer cells, and is expressed on leukocytes, germ cells and neuronal stem cells in both mouse and human. Lex is thought to enhance cell adhesion, either directly or by promoting integrin activation.

Alternate Names
CD 15; CD15; CD-15; Lewis X; Lewis-X; LeX; SSEA-1; stage-specific embryonic antigen 1

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