Human CD163 APC-conjugated Antibody

(3 citations)   
  • Species Reactivity
  • Specificity
    Detects human CD163 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 215927
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD163
    Accession # Q86VB7
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD163 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD163 APC‑conjugated Monoclonal Antibody (Catalog # FAB1607A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD163

CD163, previously called M130 or p155, is a 130‑160 kDa type I transmembrane glycoprotein that belongs to group B of the cysteine-rich scavenger receptor family (1‑3). It is essential for clearance of hemoglobin-haptoglobin (Hb-Hp) complexes in the liver, spleen and circulation (4). The human CD163 contains a 41 amino acid (aa) signal sequence, a 1009 aa extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 22 aa transmembrane segment, and a 39‑84 aa cytoplasmic region (1). The third SRCR domain is crucial for calcium-dependent binding of hemoglobin/haptoglobin complexes (3). Three splice forms (isoforms 2, 3 and 4) vary within their intracellular regions (1, 5), while one isoform (# 4) also has a 34 aa insert between SRCR domains 5 and 6 within the ECD. While all are expressed, isoform 3 is the most abundant, being generally expressed on the cell surface and most active in endocytosis (5). An approximately 130 kDa soluble form of human CD163 (sCD163) is assumed to contain virtually all of the ECD, which shares 74%, 75%, 84%, 86%, 86% and 87% aa identity with mouse, rat, bovine, equine, porcine and canine CD163 ECD, respectively (6, 7). It is released from the cell surface by proteolysis after oxidative stress or inflammatory stimuli, including bacterial endotoxins and activation of the Toll-like receptors TLR2 or TLR5 (7‑10). Expression of CD163 is constitutive, and induced by glucocorticoids, IL‑10, IL‑6 or endotoxin on circulating monocytes, tissue macrophages, and at low levels on monocyte-derived dendritic cells (1, 2, 11, 12). In addition to clearing Hb‑Hp complexes, CD163 is also a scavenger receptor for free Hb (if Hp is depleted) and TWEAK (TNF-like weak inducer of apoptosis), and can function as an erythroblast adhesion receptor (4, 13‑15).

  • References:
    1. Law, S.K.A. et al. (1993) Eur. J. Immunol. 23:2320.
    2. Sulahian, T.H. et al. (2000) Cytokine 12:1312.
    3. Madsen, M. et al. (2004) J. Biol. Chem. 279:51561.
    4. Kristiansen, M. et al. (2001) Nature 409:198.
    5. Nielsen, M.J. et al. (2006) J. Leukoc. Biol. 79:837.
    6. Moller, H.J. et al. (2002) Blood 99:378.
    7. Droste, A. et al. (1999) Biochem. Biophys. Res. Commun. 256:110.
    8. Hintz, K. A. et al. (2002) J. Leukoc. Biol. 72:711.
    9. Weaver, L.K. et al. (2006) J. Leukoc. Biol. 80:26.
    10. Timmerman, M. and P. Hogger (2005) Free Radic. Biol. Med. 39:98.
    11. Buechler, C. et al. (2000) J. Leukoc. Biol. 67:97.
    12. Pulford, K.A. et al. (1989) J. Clin. Pathol. 42:414.
    13. Schaer, D.J. et al. (2006) Blood 107:373.
    14. Bover, L.C. et al. (2007) J. Immunol. 178:8183.
    15. Fabriek, B.O. et al. (2007) Blood 109:5223.
  • Entrez Gene IDs:
    9332 (Human); 93671 (Mouse); 312701 (Rat)
  • Alternate Names:
    CD163 antigenscavenger receptor cysteine-rich type 1 protein M130; CD163 molecule; CD163; GHI/61; HbSR; Hemoglobin scavenger receptor; M130; M130macrophage-associated antigen; MM130; RM3/1
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. Prolonged neutrophil dysfunction after Plasmodium falciparum malaria is related to hemolysis and heme oxygenase-1 induction.
    Authors: Cunnington, Aubrey J, Njie, Madi, Correa, Simon, Takem, Ebako N, Riley, Eleanor, Walther, Michael
    J Immunol, 2012;189(11):5336-46.
    Species: Human
    Sample Type: whole cells
    Application: Flow
  2. Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2.
    Authors: Johnson TR, McLellan JS, Graham BS
    J. Virol., 2012;86(3):1339-47.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology.
    Authors: Shantsila E, Wrigley B, Tapp L, Apostolakis S, Montoro-Garcia S, Drayson MT, Lip GY
    J. Thromb. Haemost., 2011;9(5):1056-66.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 5  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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