Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Immunohistochemistry, Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 215927
View all CD163 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD163
Gly46-Ser1050
Accession # Q86VB7

Specificity

Detects human CD163 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD163 PE‑conjugated Antibody

Detection of CD163 antibody in Human Blood Monocytes antibody by Flow Cytometry.

Detection of CD163 in Human Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes were stained with Mouse Anti-Human CD163 PE-conjugated Monoclonal Antibody (Catalog # FAB1607P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of Human CD163 by Flow Cytometry

Detection of Human CD163 by Flow Cytometry

Phenotypic characterization of LPS- and IL-10-stimulated macrophages derived from human CD14+ peripheral blood monocytes. a Representative images of actin and tubulin stainings of LPS- and IL-10-stimulated macrophages polarized in absence of other external stimuli (mac) or in the presence of 10ng/ml LPS (LPSmac) or IL-10 (IL-10mac), respectively. F-actin was stained with Phalloidin-FITC (green), alpha –tubulin with a specific monoclonal antibody followed by incubation with AlexaFluor594 secondary antibody (red) and nuclei were counterstained with DAPI (blue). Scale bars represent 50 μm. b Morphological differences between macrophage populations were quantified by calculating the cell aspect ratio (quotient between cell major and minor axes) of actin/tubulin stained cells. Chart reflects measurements of at least 100 cells per donor from, at least, 3 distinct donors. Bars represent mean values and flags indicate standard deviations. c Cytokine production profile of LPS- and IL-10-stimulated macrophages. Cytokine concentration was measured by ELISA in conditioned media from distinct macrophage populations. Charts indicate fold increase in IL-6, IL-10 and TNF-alpha expression, in comparison to unstimulated macrophages. Data is representative of the cytokine profile of cells derived from at least 7 different donors. Bars represent mean values and flags indicate standard deviations. d Expression of typical macrophage lineage (CD14) and polarization markers (HLA-DR and CD163) was determined by flow cytometry of unstimulated, LPS- and IL-10-stimulated macrophages. Scatter charts represent percentage of positive cells for each cell surface marker considering data obtained with cells derived from 5 different donors. *, significantly different at p < 0.05. IL-10, interleukin-10; LPS, lipopolysaccharide Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26043921), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CD163 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood monocytes

Reviewed Applications

Read 2 reviews rated 4.5 using FAB1607P in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD163

CD163, previously called M130 or p155, is a 130‑160 kDa type I transmembrane glycoprotein that belongs to group B of the cysteine-rich scavenger receptor family (1‑3). It is essential for clearance of hemoglobin-haptoglobin (Hb-Hp) complexes in the liver, spleen and circulation (4). The human CD163 contains a 41 amino acid (aa) signal sequence, a 1009 aa extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 22 aa transmembrane segment, and a 39‑84 aa cytoplasmic region (1). The third SRCR domain is crucial for calcium-dependent binding of hemoglobin/haptoglobin complexes (3). Three splice forms (isoforms 2, 3 and 4) vary within their intracellular regions (1, 5), while one isoform (# 4) also has a 34 aa insert between SRCR domains 5 and 6 within the ECD. While all are expressed, isoform 3 is the most abundant, being generally expressed on the cell surface and most active in endocytosis (5). An approximately 130 kDa soluble form of human CD163 (sCD163) is assumed to contain virtually all of the ECD, which shares 74%, 75%, 84%, 86%, 86% and 87% aa identity with mouse, rat, bovine, equine, porcine and canine CD163 ECD, respectively (6, 7). It is released from the cell surface by proteolysis after oxidative stress or inflammatory stimuli, including bacterial endotoxins and activation of the Toll-like receptors TLR2 or TLR5 (7‑10). Expression of CD163 is constitutive, and induced by glucocorticoids, IL‑10, IL‑6 or endotoxin on circulating monocytes, tissue macrophages, and at low levels on monocyte-derived dendritic cells (1, 2, 11, 12). In addition to clearing Hb‑Hp complexes, CD163 is also a scavenger receptor for free Hb (if Hp is depleted) and TWEAK (TNF-like weak inducer of apoptosis), and can function as an erythroblast adhesion receptor (4, 13‑15).

References

  1. Law, S.K.A. et al. (1993) Eur. J. Immunol. 23:2320.
  2. Sulahian, T.H. et al. (2000) Cytokine 12:1312.
  3. Madsen, M. et al. (2004) J. Biol. Chem. 279:51561.
  4. Kristiansen, M. et al. (2001) Nature 409:198.
  5. Nielsen, M.J. et al. (2006) J. Leukoc. Biol. 79:837.
  6. Moller, H.J. et al. (2002) Blood 99:378.
  7. Droste, A. et al. (1999) Biochem. Biophys. Res. Commun. 256:110.
  8. Hintz, K. A. et al. (2002) J. Leukoc. Biol. 72:711.
  9. Weaver, L.K. et al. (2006) J. Leukoc. Biol. 80:26.
  10. Timmerman, M. and P. Hogger (2005) Free Radic. Biol. Med. 39:98.
  11. Buechler, C. et al. (2000) J. Leukoc. Biol. 67:97.
  12. Pulford, K.A. et al. (1989) J. Clin. Pathol. 42:414.
  13. Schaer, D.J. et al. (2006) Blood 107:373.
  14. Bover, L.C. et al. (2007) J. Immunol. 178:8183.
  15. Fabriek, B.O. et al. (2007) Blood 109:5223.

Alternate Names

CD163, GHI/61, HbSR, M130, RM3/1

Entrez Gene IDs

9332 (Human); 93671 (Mouse); 312701 (Rat)

Gene Symbol

CD163

UniProt

Q86VB7

Additional CD163 Products

Product Documents for Human CD163 PE‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD163 PE‑conjugated Antibody

For research use only

Citations for Human CD163 PE‑conjugated Antibody

Customer Reviews for Human CD163 PE‑conjugated Antibody (2)

4.5 out of 5
2 Customer Ratings
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  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 21240265
    Species: Human
    Verified Customer | Posted 01/21/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 23431165
    Species: Human
    Verified Customer | Posted 01/21/2015

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FAQs for Human CD163 PE‑conjugated Antibody

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    • Q: Does this antibody (CD163 Antibody, 215927) require permeabilization? I saw that your flow cytometry protocol calls for fixation and permeabilization, but I am looking for an antibody that can be used on intact cells.
      A: Our standard Flow protocol involves using permeabilization solution for all of our targets. It is due to our mass validation of multiple antibodies. The CD163 antibody FAB1607P is made specifically to the extracellular domain of this protein and as such, will not require the use of permeablization. For use in your experiment, you can skip the permeabilization step in our protocol.
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