Human CD163 Antibody

Catalog # Availability Size / Price Qty
MAB1607-SP
MAB1607-100
MAB1607-500
Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (3)

Human CD163 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD163 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 215927
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD163
Gly46-Ser1050
Accession # Q86VB7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CD163 (Catalog # 1607-CD) under non-reducing conditions only
Flow Cytometry
2.5 µg/106 cells
Human whole blood monocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD163

CD163, previously called M130 or p155, is a 130‑160 kDa type I transmembrane glycoprotein that belongs to group B of the cysteine-rich scavenger receptor family (1‑3). It is essential for clearance of hemoglobin-haptoglobin (Hb-Hp) complexes in the liver, spleen and circulation (4). The human CD163 contains a 41 amino acid (aa) signal sequence, a 1009 aa extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 22 aa transmembrane segment, and a 39‑84 aa cytoplasmic region (1). The third SRCR domain is crucial for calcium-dependent binding of hemoglobin/haptoglobin complexes (3). Three splice forms (isoforms 2, 3 and 4) vary within their intracellular regions (1, 5), while one isoform (# 4) also has a 34 aa insert between SRCR domains 5 and 6 within the ECD. While all are expressed, isoform 3 is the most abundant, being generally expressed on the cell surface and most active in endocytosis (5). An approximately 130 kDa soluble form of human CD163 (sCD163) is assumed to contain virtually all of the ECD, which shares 74%, 75%, 84%, 86%, 86% and 87% aa identity with mouse, rat, bovine, equine, porcine and canine CD163 ECD, respectively (6, 7). It is released from the cell surface by proteolysis after oxidative stress or inflammatory stimuli, including bacterial endotoxins and activation of the Toll-like receptors TLR2 or TLR5 (7‑10). Expression of CD163 is constitutive, and induced by glucocorticoids, IL‑10, IL‑6 or endotoxin on circulating monocytes, tissue macrophages, and at low levels on monocyte-derived dendritic cells (1, 2, 11, 12). In addition to clearing Hb‑Hp complexes, CD163 is also a scavenger receptor for free Hb (if Hp is depleted) and TWEAK (TNF-like weak inducer of apoptosis), and can function as an erythroblast adhesion receptor (4, 13‑15).

References
  1. Law, S.K.A. et al. (1993) Eur. J. Immunol. 23:2320.
  2. Sulahian, T.H. et al. (2000) Cytokine 12:1312.
  3. Madsen, M. et al. (2004) J. Biol. Chem. 279:51561.
  4. Kristiansen, M. et al. (2001) Nature 409:198.
  5. Nielsen, M.J. et al. (2006) J. Leukoc. Biol. 79:837.
  6. Moller, H.J. et al. (2002) Blood 99:378.
  7. Droste, A. et al. (1999) Biochem. Biophys. Res. Commun. 256:110.
  8. Hintz, K. A. et al. (2002) J. Leukoc. Biol. 72:711.
  9. Weaver, L.K. et al. (2006) J. Leukoc. Biol. 80:26.
  10. Timmerman, M. and P. Hogger (2005) Free Radic. Biol. Med. 39:98.
  11. Buechler, C. et al. (2000) J. Leukoc. Biol. 67:97.
  12. Pulford, K.A. et al. (1989) J. Clin. Pathol. 42:414.
  13. Schaer, D.J. et al. (2006) Blood 107:373.
  14. Bover, L.C. et al. (2007) J. Immunol. 178:8183.
  15. Fabriek, B.O. et al. (2007) Blood 109:5223.
Entrez Gene IDs
9332 (Human); 93671 (Mouse); 312701 (Rat)
Alternate Names
CD_antigen: CD163; CD163 molecule; CD163; GHI/61; HbSR; Hemoglobin scavenger receptor; M130; macrophage-associated antigen; MM130; RM3/1; SCARI1; scavenger receptor cysteine-rich type 1 protein M130; sCD163; Soluble CD163

Product Datasheets

Citations for Human CD163 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Induction of paracrine signaling in metastatic melanoma cells by PPAR? agonist rosiglitazone activates stromal cells and enhances tumor growth
    Authors: C Pich, P Meylan, B Mastelic-G, NT Nguyen, R Loyon, BK Trang, H Moser, C Moret, C Goepfert, J Hafner, MP Levesque, P Romero, C Jandus, L Michalik
    Cancer Res., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Human fetal dendritic cells promote prenatal T-cell immune suppression through arginase-2
    Authors: N McGovern, A Shin, G Low, D Low, K Duan, LJ Yao, R Msallam, I Low, NB Shadan, HR Sumatoh, E Soon, J Lum, E Mok, S Hubert, P See, EH Kunxiang, YH Lee, B Janela, M Choolani, CNZ Mattar, Y Fan, TKH Lim, DK Hong, KK Tan, JKC Tam, C Schuster, A Elbe-Bürge, XN Wang, V Bigley, M Collin, M Haniffa, A Schlitzer, M Poidinger, S Albani, A Larbi, EW Newell, JKY Chan, F Ginhoux
    Nature, 2017;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  3. Jagged1 Instructs Macrophage Differentiation in Leprosy
    PLoS Pathog, 2016;12(8):e1005808.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Endoneurial macrophages induce perineural invasion of pancreatic cancer cells by secretion of GDNF and activation of RET tyrosine kinase receptor.
    Authors: Cavel O, Shomron O, Shabtay A, Vital J, Trejo-Leider L, Weizman N, Krelin Y, Fong Y, Wong R, Amit M, Gil Z
    Cancer Res, 2012;72(22):5733-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Glioma-associated cytomegalovirus mediates subversion of the monocyte lineage to a tumor propagating phenotype.
    Authors: Dziurzynski K, Wei J, Qiao W, Hatiboglu MA, Kong LY, Wu A, Wang Y, Cahill D, Levine N, Prabhu S, Rao G, Sawaya R, Heimberger AB
    Clin. Cancer Res., 2011;17(14):4642-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. The class A scavenger receptor, macrophage receptor with collagenous structure, is the major phagocytic receptor for Clostridium sordellii expressed by human decidual macrophages.
    Authors: Thelen T, Hao Y, Medeiros AI
    J. Immunol., 2010;185(7):4328-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Human uterine epithelial cell secretions regulate dendritic cell differentiation and responses to TLR ligands.
    Authors: Ochiel DO, Ghosh M, Fahey JV
    J. Leukoc. Biol., 2010;88(3):435-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Generation of mature human myelomonocytic cells through expansion and differentiation of pluripotent stem cell-derived lin-CD34+CD43+CD45+ progenitors.
    Authors: Choi KD, Vodyanik MA, Slukvin II
    J. Clin. Invest., 2009;119(9):2818-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human CD163 Antibody

Average Rating: 3.7 (Based on 3 Reviews)

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Human CD163 Antibody
By Anonymous on 03/11/2019
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human CD163 Antibody
By Anonymous on 11/20/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Human CD163 Antibody
By Anonymous on 11/10/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human