Detection of CD177 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Goat Anti-Human CD177 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3505P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Human CD177 is a 50-65 kDa member of the uPAR/CD59/Ly6
superfamily of proteins. It contains two uPAR/Ly6 domains (vs. four in mouse),
and is expressed as a GPI-linked glycoprotein. Within common regions, human and
mouse CD177 share 55% amino acid (aa) sequence identity. CD177 is also known as
NB1 and PVR1, the difference between them being four single aa substitutions at
positions 3 (Pro-to-Ala), 119 (Phe-to-Leu), 323 (Gln-to-Arg), and 379
(Ser-to-Phe), NB1 to PVR1, respectively. It has been suggested that the PVR1
isoform appears under stress or pathological conditions. CD117 expression is restricted to neutrophils
and neutrophil precursors, becoming more widespread with maturity. It is
typically found on 45-65% of circulating neutrophils. On the cell surface,
CD117 apparently forms a complex with PR3 (protease 3) and Mac-1 (CD11b:CD18),
whereupon it both presents PR3 externally, and binds to CD31/PECAM1. On
vascular endothelial cells, its interaction with CD31 mediates neutrophil
adhesion to the vascular wall and neutrophil transmigration, apparently through
a downregulation of CD31 phosphorylation. Notably, the ability of CD117 to
facilitate transmigration appears to be a function of the isoform of CD31 to
which it binds. In this regard, a Ser at
position 536 in the sixth Ig domain of CD31 is associated with swift neutrophil
exit from the vasculature.
Polycythemia Rubra Vera Protein 1
Entrez Gene IDs:
57126 (Human); 68891 (Mouse)
CD177 antigenNB1 GP; CD177 molecule; CD177; HNA-2a; HNA2Acell surface receptor; Human neutrophil alloantigen 2a; NB1 GP; NB1PRV-1; polycythemia rubra vera 1; Polycythemia rubra vera protein 1; PRV1; PRV1NB1 glycoprotein
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