Human CD177 PE-conjugated Antibody

FAB3505P has been discontinued. View all CD177 products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human CD177/PRV1 in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD177/PRV1
    Leu22-Gly407
    Accession # Q8N6Q3
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD177 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Goat Anti-Human CD177 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3505P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD177
Human CD177 is a 50-65 kDa member of the uPAR/CD59/Ly6 superfamily of proteins. It contains two uPAR/Ly6 domains (vs. four in mouse), and is expressed as a GPI-linked glycoprotein. Within common regions, human and mouse CD177 share 55% amino acid (aa) sequence identity. CD177 is also known as NB1 and PVR1, the difference between them being four single aa substitutions at positions 3 (Pro-to-Ala), 119 (Phe-to-Leu), 323 (Gln-to-Arg), and 379 (Ser-to-Phe), NB1 to PVR1, respectively. It has been suggested that the PVR1 isoform appears under stress or pathological conditions. CD117 expression is restricted to neutrophils and neutrophil precursors, becoming more widespread with maturity. It is typically found on 45-65% of circulating neutrophils. On the cell surface, CD117 apparently forms a complex with PR3 (protease 3) and Mac-1 (CD11b:CD18), whereupon it both presents PR3 externally, and binds to CD31/PECAM1. On vascular endothelial cells, its interaction with CD31 mediates neutrophil adhesion to the vascular wall and neutrophil transmigration, apparently through a downregulation of CD31 phosphorylation. Notably, the ability of CD117 to facilitate transmigration appears to be a function of the isoform of CD31 to which it binds. In this regard, a Ser at position 536 in the sixth Ig domain of CD31 is associated with swift neutrophil exit from the vasculature.
  • Long Name:
    Polycythemia Rubra Vera Protein 1
  • Entrez Gene IDs:
    57126 (Human); 68891 (Mouse)
  • Alternate Names:
    CD177 antigenNB1 GP; CD177 molecule; CD177; HNA-2a; HNA2Acell surface receptor; Human neutrophil alloantigen 2a; NB1 GP; NB1PRV-1; polycythemia rubra vera 1; Polycythemia rubra vera protein 1; PRV1; PRV1NB1 glycoprotein
Related Research Areas

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