Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 396444
View all CD20 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human MS4A1/CD20
Met1-Pro297
Accession # P11836

Specificity

Detects human MS4A1/CD20. Stains human MS4A1/CD20 transfectants but not irrelevant transfectants in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD20 PE‑conjugated Antibody

Detection of MS4A1/CD20 antibody in HEK293 Human Cell Line Transfected with Human MS4A1/CD20 and eGFP antibody by Flow Cytometry.

Detection of MS4A1/CD20 in HEK293 Human Cell Line Transfected with Human MS4A1/CD20 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human MS4A1/CD20 and eGFP was stained with and either (A) Mouse Anti-Human MS4A1/CD20 PE-conjugated Monoclonal Antibody (Catalog # FAB4225P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Detection of MS4A1/CD20 antibody in Human Peripheral Blood Lymphocytes antibody by Flow Cytometry.

Detection of MS4A1/CD20 in Human Peripheral Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human MS4A1/CD20 PE-conjugated Monoclonal Antibody (Catalog # FAB4225P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD20 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood lymphocytes and HEK293 human embryonic kidney cell line transfected with human MS4A1/CD20 and eGFP

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD20

CD20 is a 297 amino acid (aa) non-glycosylated type III membrane protein that is the founding member of the MS4A tetraspanning protein family. It is expressed on pre-B, naïve and mature B lymphocytes and B-cell lymphomas. CD20 is associated with lipid rafts upon crosslinking, and appears to play a role in regulation of ion influx. The one extracellular segment of 48 aa shows 65% aa identity between human and mouse CD20.

Long Name

Cluster of Differentiation 20

Alternate Names

B1, Bp35, CD20, LEU-16, Ly-44, MS4A1, S7

Entrez Gene IDs

931 (Human); 12482 (Mouse); 25316 (Rat); 485430 (Canine); 102118491 (Cynomolgus Monkey)

Gene Symbol

MS4A1

UniProt

P11836

Additional CD20 Products

Product Documents for Human CD20 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD20 PE‑conjugated Antibody

For research use only

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