Human CD20 mFluor™ Violet 450-Conjugated Antibody

Catalog #: FAB4225MFV450 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB4225MFV450-100
Detection of CD20 in PBMC lymphocytes by Flow Cytometry
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Human CD20 mFluor™ Violet 450-Conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD20. Stains human CD20 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG1 Clone # 396444
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human CD20
Met1-Pro297
Accession # P11836
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
mFluor Violet 450 SE(Excitation= nm, Emission= nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
Immunohistochemistry
CyTOF-ready

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry CD20 Antibody (396444) [mFluor Violet 450 SE] View Larger

Detection of CD20 in PBMC lymphocytes by Flow Cytometry PBMC lymphocytes were stained with Mouse Anti-Human CD19 PE‑conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human CD20 mFluor™ Violet 450-Conjugated Monoclonal Antibody (Catalog # FAB4225MFV450) or (B) unstained. View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD20

CD20 is a 297 amino acid (aa) non-glycosylated type III membrane protein that is the founding member of the MS4A tetraspanin protein family. It is expressed on pre-B, naïve and mature B lymphocytes and B-cell lymphomas. CD20 is associated with lipid rafts upon crosslinking, and appears to play a role in regulation of ion influx. The one extracellular segment of 48 aa shows 65% aa sequence identity between human and mouse CD20.

Long Name
Cluster of Differentiation 20
Entrez Gene IDs
931 (Human); 12482 (Mouse); 25316 (Rat); 485430 (Canine); 102118491 (Cynomolgus Monkey)
Alternate Names
B1; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; Bp35MGC3969; CD20 antigen; CD20 receptor; CD20; CD20S7; CVID5; LEU-16; Leukocyte surface antigen Leu-16; Ly-44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MS4A1; MS4A2; S7

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Product Specific Notices

mFluorTM is a trademark of AAT Bioquest, Inc.

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