Human CD30 Ligand/TNFSF8 PE-conjugated Antibody

(1 Review)
  • Species Reactivity
  • Specificity
    Detects human CD30 Ligand/TNFSF8 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CD27 Ligand, recombinant mouse CD30 Ligand, or rhCD40 Ligand is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 116614
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD30 Ligand/TNFSF8
    Accession # P32971
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD30 Ligand/TNFSF8 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 50 ng/mL PMA and 200 ng/mL Calcium Ionomycin overnight were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human CD30 Ligand/TNFSF8 PE‑conjugated Monoclonal Antibody (Catalog # FAB1028P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD30 Ligand/TNFSF8

CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells. The specific receptor for CD30L is CD30/TNFRSF8, a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkin's and Reed-Sternberg cells using the monoclonal antibody Ki-1. CD30 is also expressed on different non-Hodgkin's lymphomas, virus-infected T and B cells, and on normal T and B cells after activation. Among T cells, CD30 is preferentially expressed on a subset of T cells producing Th2-type cytokines and on CD4+/CD8+ thymocytes that co-express CD45RO and IL-4 receptor. CD30 ligation by CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation and cell death by apoptosis. CD30 can act as a costimulatory molecule in thymic negative selection and may also play a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. Human and mouse CD30 ligand cDNAs share 70% sequence homology.

  • References:
    1. Brunangelo, F. et al. (1995) Blood 85:1.
    2. Gruss, H.-J. and F. Herrmann (1996) Leukemia and Lymphoma 20:397.
    3. Chiarle, R. et al. (1999) J. Immunol. 163:194.
  • Entrez Gene IDs:
    944 (Human); 21949 (Mouse)
  • Alternate Names:
    CD153 antigen; CD153; CD30 antigen ligand; CD30-L; CD30LCD30 ligand; CD30LGMGC138144; TNFSF8; tumor necrosis factor (ligand) superfamily, member 8; tumor necrosis factor ligand superfamily member 8
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B PE-conjugated Antibody

Ctrl IC0041P 12  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 4 (Based on 1 review)

Have you used Human CD30 Ligand/TNFSF8 PE-conjugated Antibody?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Human CD30 Ligand/TNFSF8 PE-conjugated Antibody.

We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

Filter by Ratings
Very Good
All Ratings
Filter by Applications
All Applications
Filter by Species
All Species

Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Human CD30 Ligand/TNFSF8 PE‑conjugated Antibody FAB1028P
Very Good
 Flow Human Anonymous 01/20/2016
Image Details
Flow Cytometry Human CD30 Ligand/TNFSF8 PE‑conjugated Antibody FAB1028P
Flow Cytometry: Human CD30 Ligand/TNFSF8 PE‑conjugated Antibody [FAB1028P]


ApplicationFlow Cytometry
Sample TestedPeripheral blood mononuclear cells (PBMCs)

Order Details

Contact Information

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee


Customer Information