Human CD300e/LMIR6 Antibody

MAB2705 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2705
MAB2705-SP

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Human CD300e/LMIR6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD300e/LMIR6 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human LMIR1, 2, 3, 4, or 5 is observed.

Source

Monoclonal Rat IgG_2A Clone # 233810

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Y3 rat myeloid cell line transfected with human CD300e/LMIR6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human CD300e/LMIR6 under non-reducing conditions only

Flow Cytometry

2.5 µg/10⁶ cells

Human whole blood monocytes

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD300e/LMIR6

LMIR6 (Leukocyte Mono Ig-like Receptor 6), also known as IREM-2, is a transmembrane activating receptor expressed on mature myeloid cells.

Entrez Gene IDs

342510 (Human); 217306 (Mouse)

Alternate Names

CD300 antigen like family member E; CD300 antigen-like family member E; CD300e antigenPIgR-2; CD300e molecule; CD300e; CD300LE; CD300LEPIgR2; CLM2; CLM-2; CLM2CMRF35-A5; CMRF35A5; CMRF35-like molecule 2; Immune receptor expressed on myeloid cells 2; IREM-2; IREM2IREM-2; LMIR6; Poly-Ig receptor 2; Polymeric immunoglobulin receptor 2