Detects human CD39/ENTPD1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CD39, recombinant human CD39L2, 3, or 4 is observed.
Monoclonal Mouse IgG1 Clone # 498403
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human CD39/ENTPD1
Accession # P49961
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols
are available in the Technical Information section on our website.
Detection of CD39/ENTPD1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD39/ENTPD1 PE‑conjugated Monoclonal Antibody (Catalog # FAB4397P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1) is an integral membrane protein with an extracellular active site. recombinant human (rh) NTPDase-1 was expressed as a protein lacking its N- and C-terminal transmembrane domains, resulting in the secretion of the soluble rhNTPDase-1 ectodomain. NTPDase-1 was originally described as CD39, a B lymphocyte cell surface marker, but it is also present on the surface of natural killer cells, T cells, and some endothelial cells. NTPDase-1 hydrolyzes the beta - and gamma phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides,
NTPDase-1 plays a role in the regulation of purinergic signaling. NTPDase-1 is involved in the processes of thromboregulation and vascular inflammation. The administration of soluble NTPDase-1 may have therapeutic applications for the treatment of some vascular and transplantation-associated diseases.
Ecto-Nucleoside Triphosphate Diphosphohydrolase 1
Entrez Gene IDs:
953 (Human); 12495 (Mouse); 64519 (Rat)
ADPase; ATP-diphosphatase; CD39; ENTPD1; NTPD1; ATPDase; CD39 antigen; CD39EC 184.108.40.206; DKFZp686D194; DKFZp686I093; EC 3.6.1; ecto-apyrase; Ecto-apyrase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPase 1; Ecto-ATPDase 1; ectonucleoside triphosphate diphosphohydrolase 1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPDase 1; NTPDase-1
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