Detects human CD39L1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse CD39L1 and less than 1% cross-reactivity with recombinant human (rh) CD39L2, rhCD39L3, and rhCD39L4 is observed.
Polyclonal Sheep IgG
Chinese hamster ovary cell line CHO-derived recombinant human CD39L1/ENTPD2 Thr29-Asp460 Accession # Q9Y5L3
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human CD39L1/ENTPD2 by Western Blot.
Western blot shows lysates of human heart tissue. PVDF Membrane was probed with 1 µg/mL of Human CD39L1/ENTPD2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6087) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD39L1/ENTPD2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD39L1, also known as ENTPD2 and NTPDase2, is an ectonucleotidase belonging to the CD39 family. It is found on the surface of vascular adventitial cells and accessory vascular cells (1). CD39L1 is a Ca2+ and Mg2+ dependent enzyme that activates platelets by preferentially converting ATP to ADP (2). CD39L1 plays a role in regulating thrombosis and inflammation (3). It is considered to be a therapeutic target for thromboregulation and the treatment of vascular inflammation (2, 4).
Zimmermann, H. et al. 2000 Proceedings of the Second International Workshop on EctoATPases and Related Ectonucleotidases:18.
Robson, S.C. et al. 2001 Drug Dev. Res. 53:193.
Marcus, A.J. et al. 2005 Semin. Thromb. Hemost. 31:234.
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