Recognizes the epsilon -chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).
Monoclonal Mouse IgG1 Clone # UCHT1
Protein A or G purified from hybridoma culture supernatant
Human thymocytes (1)
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of CD3 epsilon in Human Blood Lymphocytes by Flow Cytometry.
Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 PE‑conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human CD3 epsilon Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB100N) or (B) Mouse IgG1 Alexa Fluor 700 Isotype Control (Catalog # IC002N). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD3 epsilon
CD3 epsilon is a signaling molecule that participates in the formation of the T Cell Receptor (TCR). The alpha beta TCR is composed of six distinct subunits. The alpha -beta disulfide-linked heterodimer recognizes antigen. This is accompanied by four CD3 subunits that transduce an intracellular signal. CD3 epsilon forms a heterodimer with both CD3 gamma and CD3δ, creating two signaling complexes. These two heterodimers are joined by a CD3 zeta zeta homodimer, generating a fully-functional TCR (2).
Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.
Have you used Human CD3 epsilon Alexa Fluor® 700-conjugated Antibody?
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