Human CD40 Ligand/TNFSF5 Biotinylated Antibody

Catalog #: BAF617
1 Citation Datasheet / COA / SDS

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Human CD40 Ligand/TNFSF5 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD40 Ligand in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CD40 Ligand
Glu108-Leu261
Accession # P29965

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CD40 Ligand/TNFSF5 aa 108-261 (Catalog # 6245-CL)

Flow Cytometry

2.5 µg/10⁶ cells

Human peripheral blood mononuclear cells treated with PMA and Ca²⁺ ionomycin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD40 Ligand/TNFSF5

CD40 Ligand is a type II transmembrane glycoprotein belonging to the TNF family (TNFSF5). It is expressed predominantly on activated CD4⁺ T lymphocytes and also on other hematopoietic cells. CD40 L binds to CD40 (TNFRSF5) on B lymphocytes, monocytes, dendritic cells and thymic epithelium. Engagement of CD40 by CD40 Ligand promotes B cell and dendritic cell maturation and activation.

Entrez Gene IDs

959 (Human); 21947 (Mouse); 84349 (Rat)

Alternate Names

CD154 antigen; CD154; CD40 antigen ligand; CD40 Ligand; CD40L; CD40-L; CD40LG; CD40LIGM; gp39; hCD40L; HIGM1; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TNFSF5IMD3; TRAP; TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome); tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human CD40 Ligand/TNFSF5 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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