Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry

Cited:

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all CD40 Ligand/TNFSF5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CD40 Ligand
Glu108-Leu261
Accession # P29965

Specificity

Detects human CD40 Ligand in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human CD40 Ligand/TNFSF5 Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells treated with PMA and Ca2+ ionomycin

Western Blot

0.1 µg/mL
Sample: Recombinant Human CD40 Ligand/TNFSF5 aa 108-261 (Catalog # 6245-CL)

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD40 Ligand/TNFSF5

CD40 Ligand is a type II transmembrane glycoprotein belonging to the TNF family (TNFSF5). It is expressed predominantly on activated CD4+ T lymphocytes and also on other hematopoietic cells. CD40 L binds to CD40 (TNFRSF5) on B lymphocytes, monocytes, dendritic cells and thymic epithelium. Engagement of CD40 by CD40 Ligand promotes B cell and dendritic cell maturation and activation.

Alternate Names

CD154, CD40L, CD40LG, gp39, TNFSF5, TRAP

Entrez Gene IDs

959 (Human); 21947 (Mouse); 84349 (Rat)

Gene Symbol

CD40LG

UniProt

P29965

Additional CD40 Ligand/TNFSF5 Products

Product Documents for Human CD40 Ligand/TNFSF5 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CD40 Ligand/TNFSF5 Biotinylated Antibody

For research use only

Citations for Human CD40 Ligand/TNFSF5 Biotinylated Antibody

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