Human CD40 Ligand/TNFSF5 Antibody Summary
Accession # P29965
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human CD40 Ligand/TNFSF5 by Western Blot. Western blot shows conditioned media (CM) from Th17 cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD40 Ligand/TNFSF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF617) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD40 Ligand/TNFSF5 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of Human CD40 Ligand/TNFSF5 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cells, Human Platelets and Th1 CD4+. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD40 Ligand/TNFSF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF617) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for CD40 Ligand/TNFSF5 at approximately 17 and 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD40 Ligand/TNFSF5
CD40 ligand, CD40L (also known as CD154, TRAP or gp39), is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF family, CD40L is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, like NK cells, mast cells, basophils and eosinophils. Human CD40L shares 78% amino acid identity with its murine counterpart. The receptor of CD40L is CD40, a type I transmembrane glycoprotein belonging to the TNF receptor family. CD40 is expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelium. Although all monomeric, dimeric and trimeric forms of soluble CD40L can bind to CD40, the trimeric form of soluble CD40L has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members. CD40L mediates a range of activities on B cells including induction of activation-associated surface antigen, entry into cell cycle, isotype switching and Ig secretion and memory generation. CD40-CD40L interaction also plays important roles in monocyte activation and dendritic cell maturation.
- Armitage, R.J. et al. (1992) Nature 357:80.
- Hollenbaugh, D. et al. (1992) EMBO J. 11:4313.
- Spriggs, M.K. et al. (1992) J. Exp. Med. 176:1543.
- Fanslow, W.C. et al. (1994) Seminars in Immunology 6:267.
- Kooten, C.V. and J. Banchereau (2000) J. Leukoc. Biol. 67:2.
Citations for Human CD40 Ligand/TNFSF5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
Filter your results:
Heme stimulates platelet mitochondrial oxidant production to induce targeted granule secretion
Authors: GK Annarapu, D Nolfi-Done, M Reynolds, Y Wang, L Kohut, B Zuckerbrau, S Shiva
Redox Biology, 2021;48(0):102205.
Sample Types: Cell Culture Supernates
Applications: Dot Blot
CD40L expressed from the canarypox vector, ALVAC, can boost immunogenicity of HIV-1 canarypox vaccine in mice and enhance the in vitro expansion of viral specific CD8+ T cell memory responses from HIV-1-infected and HIV-1-uninfected individuals.
Authors: Liu J, Yu Q, Stone GW, Yue FY, Ngai N, Jones RB, Kornbluth RS, Ostrowski MA
Sample Types: Recombinant Protein
Applications: Western Blot
Cutting edge: nonproliferating mature immune cells form a novel type of organized lymphoid structure in idiopathic pulmonary fibrosis.
Authors: Marchal-Somme J, Uzunhan Y, Marchand-Adam S, Valeyre D, Soumelis V, Crestani B, Soler P
J. Immunol., 2006;176(10):5735-9.
Sample Types: Whole Tissue
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human CD40 Ligand/TNFSF5 Antibody
There are currently no reviews for this product. Be the first to review Human CD40 Ligand/TNFSF5 Antibody and earn rewards!
Have you used Human CD40 Ligand/TNFSF5 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image