Detects human CD40 Ligand/TNFSF5 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse CD40 Ligand is observed and less than 1% cross-reactivity with recombinant human (rh) CD27 Ligand and rhCD30 Ligand is observed.
Polyclonal Goat IgG
E. coli-derived recombinant human CD40 Ligand/TNFSF5 Glu108-Leu261 Accession # P29965
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
2.5 µg/106 cells
Human peripheral blood mononuclear cells treated with PMA and Ca2+ ionomycin
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Mouse CD40 Ligand/TNFSF5 by Western Blot. Western blot shows lysates of CTLL‑2 mouse cytotoxic T cell line and THP‑1 human acute monocytic leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human CD40 Ligand/TNFSF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF617) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD40 Ligand/TNFSF5 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD40 Ligand/TNFSF5
CD40 ligand, CD40L (also known as CD154, TRAP or gp39), is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF family, CD40L is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, like NK cells, mast cells, basophils and eosinophils. Human CD40L shares 78% amino acid identity with its murine counterpart. The receptor of CD40L is CD40, a type I transmembrane glycoprotein belonging to the TNF receptor family. CD40 is expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelium. Although all monomeric, dimeric and trimeric forms of soluble CD40L can bind to CD40, the trimeric form of soluble CD40L has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members. CD40L mediates a range of activities on B cells including induction of activation-associated surface antigen, entry into cell cycle, isotype switching and Ig secretion and memory generation. CD40-CD40L interaction also plays important roles in monocyte activation and dendritic cell maturation.
Armitage, R.J. et al. (1992) Nature 357:80.
Hollenbaugh, D. et al. (1992) EMBO J. 11:4313.
Spriggs, M.K. et al. (1992) J. Exp. Med. 176:1543.
Fanslow, W.C. et al. (1994) Seminars in Immunology 6:267.
Kooten, C.V. and J. Banchereau (2000) J. Leukoc. Biol. 67:2.
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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