Detects human CD44 v6 in Western blots. By flow cytometry analysis using a panel of CD44-transfected COS cells (9), monoclonal antibody clone 2F10 was deduced to be specific for human CD44 protein isoforms containing variant exon 6 (CD44 v6).
Monoclonal Mouse IgG1 Clone # 2F10
Protein A or G purified from ascites
Recombinant human CD44 v3-10
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 488
5 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD44 v6 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD44 v6 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB3660G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration (1-3). Human CD44 has a 20 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 325-530 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. Within the stem, ten variably spliced exons (v1-10, exons 6-15) produce multiple protein isoforms (1-3). The standard or hematopoietic form, CD44H, does not include the variable segments (1-3). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (1, 3). With variable N- and O-glycosylation and splicing within the stalk, CD44 can range from 80 to 200 kDa (1). Within the N-terminal invariant portion of the ECD (aa 21‑220), human CD44 shares 76%, 76%, 86%, 83% and 79% aa sequence identity with corresponding mouse, rat, equine, canine and bovine CD44, respectively. The many reported functions of CD44 fall within three categories (1). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a “platform” for growth factors and metalloproteinases. Second, CD44 can function as a co-receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form (4) or may be cleaved at multiple sites by either membrane-type matrix metalloproteinases, or ADAM proteases to produce soluble ectodomains (5, 6). The cellular portion may then undergo gamma secretase-dependent intramembrane cleavage to form an A beta -like transmembrane portion and a cytoplasmic signaling portion that affects gene expression (7, 8). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 5).
Ponta, H. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:33.
Screaton, G.R. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12160.
Lynch, K.W. (2004) Nat. Rev. Immunol. 4:931.
Yu, Q. and B.P. Toole (1996) J. Biol. Chem. 271:20603.
Nagano, O. and H. Saya (2004) Cancer Sci. 95:930.
Nakamura, H. et al. (2004) Cancer Res. 64:876.
Murakami, D. et al. (2003) Oncogene 22:1511.
Lammich, S. et al. (2002) J. Biol. Chem. 277:44754.
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