Human CD44 v6 PE-conjugated Antibody

(4 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human CD44 v6 in Western blots. By flow cytometry analysis using a panel of CD44-transfected COS cells (9), monoclonal antibody clone 2F10 was deduced to be specific for human CD44 protein isoforms containing variant exon 6 (CD44 v6).
  • Source
    Monoclonal Mouse IgG1 Clone # 2F10
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    Recombinant human CD44 v3-10
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD44 v6 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD44 v6 PE‑conjugated Monoclonal Antibody (Catalog # FAB3660P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD44
CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration (1-3). Human CD44 has a 20 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 325-530 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. Within the stem, ten variably spliced exons (v1-10, exons 6-15) produce multiple protein isoforms (1-3). The standard or hematopoietic form, CD44H, does not include the variable segments (1-3). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (1, 3). With variable N- and O-glycosylation and splicing within the stalk, CD44 can range from 80 to 200 kDa (1). Within the N-terminal invariant portion of the ECD (aa 21-220), human CD44 shares 76%, 76%, 86%, 83% and 79% aa sequence identity with corresponding mouse, rat, equine, canine and bovine CD44, respectively. The many reported functions of CD44 fall within three categories (1). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a “platform” for growth factors and metalloproteinases. Second, CD44 can function as a co-receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form (4) or may be cleaved at multiple sites by either membrane-type matrix metalloproteinases, or ADAM proteases to produce soluble ectodomains (5, 6). The cellular portion may then undergo gamma secretase-dependent intramembrane cleavage to form an A beta -like transmembrane portion and a cytoplasmic signaling portion that affects gene expression (7, 8). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 5).
  • References:
    1. Ponta, H. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:33.
    2. Screaton, G.R. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12160.
    3. Lynch, K.W. (2004) Nat. Rev. Immunol. 4:931.
    4. Yu, Q. and B.P. Toole (1996) J. Biol. Chem. 271:20603.
    5. Nagano, O. and H. Saya (2004) Cancer Sci. 95:930.
    6. Nakamura, H. et al. (2004) Cancer Res. 64:876.
    7. Murakami, D. et al. (2003) Oncogene 22:1511.
    8. Lammich, S. et al. (2002) J. Biol. Chem. 277:44754.
    9. Fox, S.B. et al. (1994) Cancer Res. 54:4539.
  • Entrez Gene IDs:
    960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)
  • Alternate Names:
    CD44 antigen; CD44 molecule (Indian blood group); CD44; CD44R; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; Hyaluronate receptor; IN; LHR; MC56; MDU2; MDU2CD44 antigen (homing function and Indian blood group system); MDU3; MDU3CDW44; MIC4; MIC4MGC10468; MUTCH-I; Pgp1; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Autophagy supports generation of cells with high CD44 expression via modulation of oxidative stress and Parkin-mediated mitochondrial clearance
    Authors: KA Whelan, PM Chandramou, K Tanaka, M Natsuizaka, M Guha, S Srinivasan, DS Darling, Y Kita, S Natsugoe, JD Winkler, AJ Klein-Szan, RK Amaravadi, NG Avadhani, AK Rustgi, H Nakagawa
    Oncogene, 2017;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: Flow
  2. Coexpression of EpCAM, CD44 variant isoforms and claudin-7 in anaplastic thyroid carcinoma.
    Authors: Okada, Toshihir, Nakamura, Teruo, Watanabe, Takayuki, Onoda, Naoyoshi, Ashida, Atsuko, Okuyama, Ryuhei, Ito, Ken-ichi
    PLoS ONE, 2014;9(4):e94487.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Hypoxia regulates CD44 and its variant isoforms through HIF-1alpha in triple negative breast cancer.
    Authors: Krishnamachary B, Penet M, Nimmagadda S, Mironchik Y, Raman V, Solaiyappan M, Semenza G, Pomper M, Bhujwalla Z
    PLoS ONE, 2012;7(8):e44078.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Preferential migration of T regulatory cells induced by IL-16.
    Authors: McFadden C, Morgan R, Rahangdale S, Green D, Yamasaki H, Center D, Cruikshank W
    J. Immunol., 2007;179(10):6439-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 38  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 5 (Based on 1 review)

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Flow Cytometry Human CD44 v6 PE-conjugated Antibody FAB3660P
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Flow Cytometry Human CD44 v6 PE-conjugated Antibody FAB3660P
Flow Cytometry: Human CD44 v6 PE-conjugated Antibody [FAB3660P]

Summary

ApplicationFlow Cytometry
Sample TestedMCF-7 human breast cancer cell line
SpeciesHuman

Other Experimental Details

Other Experimental Details10^6 MCF-7 cells were incubated for 30 minutes at 4ºC with 10 ul of Human CD44v6 PE-conjugated antibody (red), or control antibody (black). Blocking: 1% BSA. Unfixed cells.

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