Human CD5 Biotinylated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CD5 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2A Clone # 205919
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD5
    Arg25-Asn371
    Accession # P06127
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD5 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD5 Biotinylated Monoclonal Antibody (Catalog # FAB1636B, filled histogram) or isotype control antibody (Catalog # IC003B, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD5

CD5, also known as Leu-1, is a type I transmembrane protein belonging to the scavenger receptor cysteine-rich (SRCR) superfamily. It is expressed on thymocytes, T cells and the B1a subpopulation of B cells. CD5 binds the C-type lectin, CD72, and modulates signaling through the antigen receptors on T and B cells. CD5 has been shown to provide either positive or negative co-stimulatory signals depending on cell type to regulate immune responses.

  • References:
    1. Ledbetter, J.A. et al. (1985) J. Immunol. 135:2331.
    2. Damle, N.K. et al. (1988) J. Immunol. 140:1753.
  • Entrez Gene IDs:
    921 (Human); 12507 (Mouse)
  • Alternate Names:
    CD5 antigen (p56-62); CD5 antigen; CD5 molecule; CD5; LEU1T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; T1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Biotinylated Isotype Control

Ctrl IC003B
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 4
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Streptavidin-Phycoerythrin

Flow F0040 5
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Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 1
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 3
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NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
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NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Avidin-HRP

WB A-115 1
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NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
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Streptavidin-Allophycocyanin

Flow F0050 1
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Avidin-Fluorescein

Flow F0030
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Supplemental Cell Selection Products
Description Application Cat# Citations Images  

MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Supplemental ELISA Products
Description Application Cat# Citations Images  

Streptavidin-HRP (set of 5 vials enough for 75 plates total)

ELISA DY998 36
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EvenCoat Streptavidin Coated Plates, 15 Pack

ELISA CP004
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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 2
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