Human CD5 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CD5 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # 205919
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD5
    Arg25-Asn371
    Accession # P06127
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human CD5 under non-reducing conditions only
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Agonist Activity
    1.5-6 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Human CD5 Antibody Enhances IL‑2 Secretion in Human T Cells.

Human CD5 Monoclonal Antibody enhances IL-2 secretion in human T cells in the presence of sub-optimal amounts of Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100) and Human CD28 Monoclonal Antibody (Catalog # MAB342), in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA Kit (Catalog # D2050). The ED50 for this effect is typically 1.5-6 μg/mL.

Detection of CD5 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD5 Monoclonal Antibody (Catalog # MAB1636, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD5

CD5, also known as Leu-1, is a type I transmembrane protein belonging to the scavenger receptor cysteine-rich (SRCR) superfamily. It is expressed on thymocytes, T cells and the B1a subpopulation of B cells. CD5 binds the C-type lectin, CD72, and modulates signaling through the antigen receptors on T and B cells. CD5 has been shown to provide either positive or negative co-stimulatory signals depending on cell type to regulate immune responses.

  • References:
    1. Ledbetter, J.A. et al. (1985) J. Immunol. 135:2331.
    2. Damle, N.K. et al. (1988) J. Immunol. 140:1753.
  • Entrez Gene IDs:
    921 (Human); 12507 (Mouse)
  • Alternate Names:
    CD5 antigen (p56-62); CD5 antigen; CD5 molecule; CD5; LEU1T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; T1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Isotype Control

Ctrl MAB003 32  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Mouse IgG2A Antibody

Flow MAB0032  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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