Human CD6 Alexa Fluor® 488-conjugated Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects recombinant human CD6 in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD6
    His18-Glu398
    Accession # Q8WWJ7
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD6 in Human Whole Blood Lymphocytes by Flow Cytometry. Human whole blood lymphocytes were stained with Goat Anti-Human CD6 Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB627G, filled histogram) or isotype control antibody (Catalog # IC108G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD6
Human CD6 is a 105 kDa (unphosphorylated) -130 kDa (hyperphosphorylated) glycoprotein Group B member of the SRCR (Scavenger Receptor Cys-Rich) superfamily of molecules. It has a restricted expression pattern, being limited to select CD56dim NK cells, B1a B cells (IgM producers), thymocytes and virtually all resting T cells. Mature CD6 is a type I transmembrane protein 651 amino acids (aa) in length. It contains a 385 aa extracellular region (aa 19-402) that possesses three SRCR repeats, and a relatively long 245 aa cytoplasmic domain. There are at least six alternative splice forms for human CD6, four of which (plus the full-length form) contain an unspliced extracellular region. The isoform used here for immunization (SwissProt: Q8WWJ7) is one of these four, showing splicing only in the cytoplasmic region. Thus, the polyclonal antibody used here will recognize all full-length CD6 molecules, and may, by an absence of staining, suggest the presence of the two isoform variants that are missing the third SRCR repeat that lies between aa 259-361. CD6 has multiple interaction partners. It complexes with CD5 in cis, contributing to the immunological synapse; in trans, it binds to dendritic cell CD166/ALCAM on the surface of adjacent cells. CD6 is also known to bind soluble galectin-1 and -3, and to bind the PAMPs associated with pathogenic microbes. Notably, on T cells and following activation, full-length CD6 is downregulated, and a splice variant lacking the third SRCR is upregulated, abrogating any CD6:CD166 interaction. Functions attributed to CD6 include the induction of chemokine secretion by NK cells, costimulation of antigen recognition by T cells, and an antimicrobial activity attributed to an 85 kDa soluble form of CD6. Over aa 18-398, human and mouse CD6 share 68% aa sequence identity.
  • Entrez Gene IDs:
    923 (Human); 12511 (Mouse)
  • Alternate Names:
    CD6 antigenFLJ44171; CD6 molecule; CD6; T12; Tp120; TP120T-cell differentiation antigen CD6
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. CD318 is a ligand for CD6
    Authors: G Enyindah-A, Y Li, JH Ruth, DS Spassov, KE Hebron, A Zijlstra, MM Moasser, B Wang, NG Singer, H Cui, RA Ohara, SM Rasmussen, DA Fox, F Lin
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Alexa Fluor® 488-conjugated Control

Ctrl IC108G
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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