Human CD83 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human CD83.
  • Source
    Monoclonal Mouse IgG1 Clone # HB15e
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    COS-7 African green monkey SV40 transformed kidney fibroblast-like cells transfected with human CD83
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD83 in CD14+ Monocyte-Derived Dendritic Cells by Flow Cytometry. CD14+ monocyte-derived dendritic cells were stained with Mouse Anti-Human CD83 APC‑conjugated Monoclonal Antibody (Catalog # FAB1774A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD83

Human CD83 is a 40‑50 kDa member of the Siglec (or sialic-acid-binding immunoglobulin-like lectin) family of transmembrane proteins (1, 2, 3). CD83 is synthesized as a type I transmembrane glycoprotein that contains a 125 amino acid (aa) extracellular region, a 22 aa transmembrane segment, and 39 aa cytoplasmic domain. It contains one V type Ig-like domain in the extracellular region with no inhibitory cytoplasmic motif(s). Although in vitro studies suggest CD83 may form membrane-bound covalent homodimers, in vivo this does not appear to be the case (1, 4). In the extracellular region, mouse and human CD83 are 66% aa identical (1, 2, 4, 5). Relative to human, mouse CD83 is 11 aa shorter in its extracellular domain and is expressed as a 30‑35 kDa protein (1, 4, 5). Human CD83 is active in the mouse system (4). One alternate splice form has been reported. This leads to a small monomeric soluble form of 74 aa that includes aa 20‑52 and aa 164‑205 (6, 7). In human, proteolytic cleavage and solubilization of CD83 has also been suggested, and this could lead to dimeric circulating CD83 (4, 6). CD83 is a primary marker for dendritic cells (3, 6, 8). It is also found on B cells (6, 9), neutrophils (10), monocytes and macrophages (11). Except for dendritic cells, CD83 expression is often transient. CD83 binds to sialic acids on target cells (12). Membrane CD83 appears to promote T cell proliferation, particularly of CD8+ cytotoxic T cells (13, 14). Soluble CD83, however, appears to be immunosuppressive and blocks T cell activation (15, 16). On monocytes, CD83 is suggested to drive monocytes into a fibrocyte phenotype (13). A lack of membrane-expressed CD83 leads to an unusual IL-4/IL-10 producing CD4+ T cell phenotype (17).

  • References:
    1. Zhou, L-J. et al. (1992) J. Immunol. 149:735.
    2. Kozlow, E.J. et al. (1993) Blood 81:454.
    3. Fujimoto, Y and T.F. Tedder (2006) J. Med. Dent. Sci. 53:85.
    4. Lechmann, M. et al. (2005) Biochem. Biophys. Res. Commun. 329:132.
    5. Berchtold, S. et. al. (1999) FEBS Lett. 461:211.
    6. Hock, B.D. et al. (2001) Int. Immunol. 13:959.
    7. Dudziak, D. et al. (2005) J. Immunol. 174:6672.
    8. Velten, F.W. et al. (2007) Mol. Immunol. 44:1544.
    9. Cramer, S.O. et al. (2000) Int. Immunol. 12:1347.
    10. Yamashiro, S. et al. (2000) Blood 96:3958.
    11. Cao, W. et al. (2005) Biochem. J. 385:85.
    12. Scholler, N. et al. (2001) J. Immunol. 166:3865.
    13. Scholler, N. et al. (2002) J. Immunol. 168:2599.
    14. Hirano, N. et al. (2006) Blood 107:1528.
    15. Kotzor, N. et al. (2004) Immunobiology 209:129.
    16. Zinser, E. et al. (2006) Immunobiology 211:449.
    17. Garcia-Martinez, L.F. et al. (2004) J. Immunol. 173:2995.
  • Entrez Gene IDs:
    9308 (Human); 12522 (Mouse)
  • Alternate Names:
    B-cell activation protein; BL11; BL11CD83 antigen; CD83 antigen (activated B lymphocytes, immunoglobulin superfamily); CD83 molecule; CD83; Cell surface protein HB15; cell-surface glycoprotein; HB15; HB15hCD83
Related Research Areas
Isotype Controls
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Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 5  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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