Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Blockade of Receptor-ligand Interaction, Flow Cytometry, CyTOF-ready

Cited:

Neutralization, Flow Cytometry, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 131412
View all CD94 Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with human CD94 and NKG2A

Specificity

Recognizes human CD94 both in its homodimeric form and as a heterodimer with either NKG2A or NKG2C.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications for Human CD94 Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

HLA-E tetramers at 3 μg/mL were incubated with BaF/3 cells transfected with hNKG2C/CD94, and binding was monitored by flow cytometry. A 15 minute pre-incubation of the transfected cells with this antibody at a concentration of 17 μg/mL inhibited 95% of the HLA-E tetramer binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human whole blood CD56+ natural killer cells

Reviewed Applications

Read 2 reviews rated 2 using MAB1058 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CD94

The type-II transmembrane glycoprotein CD94 covalently associates with C-type lectins of the NKG2 family to yield heterodimers important in NK cell recognition of class I MHC molecules. CD94/NKG2A complexes are also found on a subset of CD8+ T cells. Expression of CD94/NKG2 heterodimers may regulate cell survival and effector functions. There are two alternatively spliced variants of CD94 that differ by 31 amino acids encoded by exon 2.

Alternate Names

CD94, KLRD1

Entrez Gene IDs

3824 (Human); 16643 (Mouse); 102119829 (Cynomolgus Monkey)

Gene Symbol

KLRD1

Additional CD94 Products

Product Documents for Human CD94 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CD94 Antibody

For research use only

Citations for Human CD94 Antibody

Customer Reviews for Human CD94 Antibody (2)

2 out of 5
2 Customer Ratings
5 Stars
0%
4 Stars
0%
3 Stars
0%
2 Stars
100%
1 Stars
0%

Have you used Human CD94 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Human CD94 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: B cell enriched peripheral blood mononuclear cells (PBMC)
    Species: Human
    Verified Customer | Posted 06/10/2022
    Red -> HEK cells (neg control) Blue -> Human PBMCs
    Human CD94 Antibody MAB1058
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
  • Human CD94 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 06/10/2022
    1. Mock transfected HEK cells 2. hCD94-transfected HEK cells
    Human CD94 Antibody MAB1058
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies