Mouse IgG Antibody

Catalog # Availability Size / Price Qty
AF007
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews

Mouse IgG Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IgG1, IgG2A, IgG2B, IgG3 heavy and light chains.  In direct ELISAs, less than 15% cross-reactivity with human IgG and rabbit IgG is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot

Use at a 1:5000 dilution in conjunction with an appropriate tertiary detection antibody.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Product Datasheets

Citations for Mouse IgG Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Autoreactive CD19+CD20- Plasma Cells Contribute to Disease Severity of Experimental Autoimmune Encephalomyelitis.
    Authors: Chen D, Ireland S, Davis L, Kong X, Stowe A, Wang Y, White W, Herbst R, Monson N
    J Immunol, 2016;196(4):1541-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISpot Development
  2. Vacuolar-ATPase isoform a2 regulates macrophages and cytokine profile necessary for normal spermatogenesis in testis.
    Authors: Jaiswal M, Katara G, Mallers T, Chaouat G, Gilman-Sachs A, Beaman K
    J Leukoc Biol, 2014;96(2):337-47.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  3. Distinct binding affinities of Mac-1 and LFA-1 in neutrophil activation.
    Authors: Li N, Mao D, Lu S, Tong C, Zhang Y, Long M
    J Immunol, 2013;190(8):4371-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  4. Vascular endothelial growth factor is crucial for erythropoietin-induced improvement of cardiac function in heart failure.
    Authors: Westenbrink BD, Ruifrok WP, Voors AA
    Cardiovasc. Res., 2010;87(1):30-9.
    Species: Rat
    Sample Types: In Vivo
    Applications: Ctrl

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Secondary Antibodies

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