Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
F0103B
Detection of MouseIgG Primary Antibody by Flow Cytometry
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Product Details
Citations (5)
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IgG primary antibodies in immunofluorescent assays. After purification by affinity chromatography, this antibody was adsorbed to eliminate human cross-reactivity and the IgG fraction was further digested with pepsin to generate F(ab')2 fragments which have reduced interactions with cell surface Fc receptors
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse IgG heavy and light chains
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein

Applications

Recommended Concentration
Sample
Flow Cytometry
Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of mouse origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of MouseIgG Primary Antibody by Flow Cytometry View Larger

Detection of MouseIgG Primary Antibody by Flow Cytometry HT1080 humanfibrosarcoma cell line was stained with Mouse Anti-Human Integrin alpha V/CD51Monoclonal Antibody (Catalog # MAB1219,filled histogram) or isotype control antibody (Catalog # MAB002,open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG SecondaryAntibody (Catalog # F0103B).

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Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Product Datasheets

Citations for Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Tacrolimus Protects Podocytes from Injury in Lupus Nephritis Partly by Stabilizing the Cytoskeleton and Inhibiting Podocyte Apoptosis.
    Authors: Liao R, Liu Q, Zheng Z, Fan J, Peng W, Kong Q, He H, Yang S, Chen W, Tang X, Yu X
    PLoS ONE, 2015;10(7):e0132724.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Paraffin embedded
  2. Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation.
    Authors: Pinto S, Martinez-Romero A, O'Connor J, Gil-Benso R, San-Miguel T, Terradez L, Monteagudo C, Callaghan R
    BMC Cancer, 2014;14(0):118.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  3. Quantification of endothelial microparticles on modified cytometric bead assay and prognosis in chest pain patients.
    Authors: Fan, Yuqi, Wang, Lianshen, Li, Yuanmin, Yin, Zhaofang, Xu, Zuojun, Wang, Changqia
    Circ J, 2014;78(1):206-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  4. The role of RGD-tagged helical rosette nanotubes in the induction of inflammation and apoptosis in human lung adenocarcinoma cells through the P38 MAPK pathway.
    Authors: Suri SS, Rakotondradany F, Myles AJ, Fenniri H, Singh B
    Biomaterials, 2009;30(17):3084-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
    Authors: Ebert EC, Mehta V
    Cell. Immunol., 2006;241(1):7-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Secondary

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Secondary Antibodies

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