Mouse F(ab)2 IgG (H+L) Fluorescein‑conjugated Antibody

R&D Systems | Catalog # F0103B

R&D Systems
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Polyclonal Goat IgG
View all IgG Secondary Antibodies »

Product Specifications

Immunogen

Mouse IgG heavy and light chains

Specificity

Detects mouse IgG primary antibodies in immunofluorescent assays. After purification by affinity chromatography, this antibody was adsorbed to eliminate human cross-reactivity and the IgG fraction was further digested with pepsin to generate F(ab')2 fragments which have reduced interactions with cell surface Fc receptors

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse F(ab)2 IgG (H+L) Fluorescein‑conjugated Antibody

Detection of Mouse IgG Primary Antibody by Flow Cytometry

Detection of Mouse IgG Primary Antibody by Flow Cytometry

HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin aV/CD51 Monoclonal Antibody (Catalog # MAB1219, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B).

Applications for Mouse F(ab)2 IgG (H+L) Fluorescein‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of mouse origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.

Reviewed Applications

Read 1 review rated 4 using F0103B in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Mouse F(ab)2 IgG (H+L) Fluorescein‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse F(ab)2 IgG (H+L) Fluorescein‑conjugated Antibody

For research use only

Citations for Mouse F(ab)2 IgG (H+L) Fluorescein‑conjugated Antibody

Customer Reviews for Mouse F(ab)2 IgG (H+L) Fluorescein‑conjugated Antibody (1)

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  • Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody
    Name: Abby Sukarto
    Application: Flow Cytometry
    Sample Tested: Bone Extracts
    Species: Human
    Verified Customer | Posted 02/13/2020
    Mouse F(ab)2 IgG (H+L) Fluorescein‑conjugated Antibody F0103B

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FAQs for Mouse F(ab)2 IgG (H+L) Fluorescein‑conjugated Antibody

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  • Q: What is the meaning of F(ab') or F(ab')2?

    A: F(ab') and F(ab')2 antibodies are fragments of antibodies that contain the variable regions of the antibody but not the Fc regions.See https://www.bio-techne.com/reagents/antibodies/antibody-validation/antibody-basics for more information on the general structure of antibodies.An antibody product that is denoted as F(ab') includes 1 F(ab) subunit and a portion of the linking region of the antibody. F(ab')2 specifies that there are 2 F(ab) subunits and the linking region.

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