Human CD96 Alexa Fluor® 532-conjugated Antibody

Catalog #: AF6199X Datasheet / COA / SDS

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AF6199X-100UG

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All CD96 Antibodies

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Human CD96 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD96 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant mouse CD96 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD96
Lys25-Asp501
Accession # NP_005807

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CD96

CD96 (also Tactile) is a 160 kDa member of the Ig-Superfamily. It is expressed on CD4⁺ and CD8⁺ T cells, plus NK cells and select B cells. Human CD96 binds to CD155 and presumably participates in NK cell killing of CD155-expressing target cells. Mature human CD96 is a 564 amino acid (aa), type I transmembrane glycoprotein. It contains a 498 aa extracellular region (aa 22-519) that contains three Ig-like domains. The two N-terminal domains are V-type (aa 38-238), while the distal domain is a C-type structure (aa 269-375). There is one isoform that shows a deletion of aa 183-192. This deletion converts the second V-type domain into an I‑like domain, and generates the most common form of CD96. An additional isoform shows the same deletion coupled to a nine aa substitution for aa 410-585. Over aa 1-536, human CD96 shares 59% aa identity with mouse CD96.

Entrez Gene IDs

10225 (Human); 84544 (Mouse); 498079 (Rat); 102121080 (Cynomolgus Monkey)

Alternate Names

CD96 antigenMGC22596; CD96 molecule; CD96; DKFZp667E2122; T cell-activated increased late expression protein; TACTILE; TACTILEincreased late expression; T-cell surface protein tactile