Detection of Human CD96 by Western Blot.
Western blot shows lysates of human Th2 cells. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD96 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6199) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD96 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD96 (also Tactile) is a 160 kDa member of the Ig-Superfamily. It is expressed on CD4+ and CD8+ T cells, plus NK cells and select B cells. Human CD96 binds to CD155 and presumably participates in NK cell killing of CD155-expressing target cells. Mature human CD96 is a 564 amino acid (aa), type I transmembrane glycoprotein. It contains a 498 aa extracellular region (aa 22-519) that contains three Ig-like domains. The two N-terminal domains are V-type (aa 38-238), while the distal domain is a C-type structure (aa 269-375). There is one isoform that shows a deletion of aa 183-192. This deletion converts the second V-type domain into an I‑like domain, and generates the most common form of CD96. An additional isoform shows the same deletion coupled to a nine aa substitution for aa 410-585. Over aa 1-536, human CD96 shares 59% aa identity with mouse CD96.
Entrez Gene IDs:
10225 (Human); 84544 (Mouse); 498079 (Rat)
CD96 antigenMGC22596; CD96 molecule; CD96; DKFZp667E2122; T cell-activated increased late expression protein; TACTILE; TACTILEincreased late expression; T-cell surface protein tactile
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