Human CEACAM-3/CD66d Antibody

Catalog # Availability Size / Price Qty
AF4166
AF4166-SP
Detection of CEACAM‑3/CD66d in Human Granulocytes by Flow Cytometry.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human CEACAM-3/CD66d Antibody Summary

Species Reactivity
Human
Specificity
Detects human CEACAM‑3/CD66d in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) CEACAM-1, rhCEACAM-5, and rhCEACAM-6 is observed and less than 5% cross-reactivity with rhCEACAM-4, rhCEACAM-7, and rhCEACAM-8 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CEACAM‑3/CD66d isoform 1
Lys35-Gly155
Accession # P40198
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CEACAM‑3/CD66d
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CEACAM‑3/CD66d in Human Granulocytes by Flow Cytometry. View Larger

Detection of CEACAM‑3/CD66d in Human Granulocytes by Flow Cytometry. Human whole blood granulocytes were stained with Sheep Anti-Human CEACAM‑3/CD66d Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4166, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-3/CD66d

CEACAM-3 (carcinoembryonic antigen-related cell adhesion molecule 3; also CD66d and CGM1) is a 31‑34 kDa member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and serves as a microbial receptor for a wide range of microorganisms. Mature human CEACAM-3 is a 218 amino acid (aa) type I transmembrane glycoprotein. Its extracellular domain (aa 35‑155) shows one V-type Ig-like domain (aa 35‑142). There are two alternate splice forms. One shows a 40 aa substitution for the C-terminal 116 amino acids (aa 137‑252), while a second shows a 31 aa substitution for the C-terminal 71 amino acids. No definitive rodent CEACAM-3 has been reported.

Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 3
Entrez Gene IDs
1084 (Human); 384557 (Mouse); 24926 (Rat)
Alternate Names
CD66d; CEA; CEACAM3 carcinoembryonic antigen-related cell adhesion molecule 3; CEACAM-3; CGM1; W264; W282

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human CEACAM-3/CD66d Antibody

There are currently no reviews for this product. Be the first to review Human CEACAM-3/CD66d Antibody and earn rewards!

Have you used Human CEACAM-3/CD66d Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review