CEACAM-3 (carcinoembryonic antigen-related cell adhesion molecule 3; also CD66d and CGM1) is a 31‑34 kDa member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and serves as a microbial receptor for a wide range of microorganisms. Mature human CEACAM-3 is a 218 amino acid (aa) type I transmembrane glycoprotein. Its extracellular domain (aa 35‑155) shows one V-type Ig-like domain (aa 35‑142). There are two alternate splice forms. One shows a 40 aa substitution for the C-terminal 116 amino acids (aa 137‑252), while a second shows a 31 aa substitution for the C-terminal 71 amino acids. No definitive rodent CEACAM-3 has been reported.
Human CEACAM‑3/CD66d Antibody
R&D Systems | Catalog # AF4166
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CEACAM‑3/CD66d isoform 1
Lys35-Gly155
Accession # P40198
Lys35-Gly155
Accession # P40198
Specificity
Detects human CEACAM‑3/CD66d in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) CEACAM-1, rhCEACAM-5, and rhCEACAM-6 is observed and less than 5% cross-reactivity with rhCEACAM-4, rhCEACAM-7, and rhCEACAM-8 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human CEACAM‑3/CD66d Antibody
Detection of CEACAM‑3/CD66d in Human Granulocytes by Flow Cytometry.
Human whole blood granulocytes were stained with Sheep Anti-Human CEACAM-3/CD66d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4166, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).
Applications for Human CEACAM‑3/CD66d Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human whole blood granulocytes
Sample: Human whole blood granulocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Human CEACAM‑3/CD66d
Sample: Recombinant Human CEACAM‑3/CD66d
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CEACAM-3/CD66d
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 3
Alternate Names
CD66d, CGM1
Gene Symbol
CEACAM3
UniProt
Additional CEACAM-3/CD66d Products
Product Documents for Human CEACAM‑3/CD66d Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human CEACAM‑3/CD66d Antibody
For research use only
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