Detects human CEACAM‑3/CD66d in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human (rh) CEACAM-1 is observed, 10% cross-reactivity with rhCEACAM-6 is observed, and 5% cross-reactivity with rhCEACAM-4 and rhCEACAM‑5 is observed.
Polyclonal Sheep IgG
Mouse myeloma cell line NS0-derived recombinant human CEACAM‑3/CD66d isoform 1 Lys35-Gly155 Accession # P40198
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Recombinant Human CEACAM‑3/CD66d
2.5 µg/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CEACAM‑3/CD66d in Human Granulocytes by Flow Cytometry. Human whole blood granulocytes were stained with Sheep Anti-Human CEACAM‑3/ CD66d Biotinylated Antigen Affinity‑purified Polyclonal Antibody (Catalog # BAF4166, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CEACAM-3 (carcinoembryonic antigen-related cell adhesion molecule 3; also CD66d and CGM1) is a 31‑34 kDa member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and serves as a microbial receptor for a wide range of microorganisms. Mature human CEACAM-3 is a 218 amino acid (aa) type I transmembrane glycoprotein. Its extracellular domain (aa 35‑155) shows one V-type Ig-like domain (aa 35‑142). There are two alternate splice forms. One shows a 40 aa substitution for the C-terminal 116 amino acids (aa 137‑252), while a second shows a 31 aa substitution for the C-terminal 71 amino acids. No definitive rodent CEACAM-3 has been reported.
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