Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all CEACAM-7 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CEACAM-7
Asn37-Phe142
Accession # Q14002

Specificity

Detects human CEACAM-7 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human CEACAM-1, -3, -4, -5, -6, and -8 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human CEACAM‑7 Biotinylated Antibody

Detection of Human CEACAM-7 antibody by Western Blot.

Detection of Human CEACAM‑7 by Western Blot.

Western blot shows lysates of HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human CEACAM-7 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4478) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CEACAM-7 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human CEACAM‑7 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: HT‑29 human colon adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CEACAM-7

CEACAM-7 (carcinoembryonic antigen cell adhesion molecule 7; also CGM2) is a 40‑45 kDa member of the CEACAM subfamily of the CEA family of proteins. It has a restricted expression pattern, being found on epithelium of the colon, appendix and pancreatic ducts. It is markedly down‑regulated in colon carcinoma. Mature human CEACAM-7 is a 209 amino acid (aa), GPI-linked glycoprotein. It contains one V-type (aa 36‑142) and one C2-type (aa 146‑233) Ig-like domain, plus a 21 aa C‑terminal propeptide. One alternate splice form exists that shows a deletion of aa 143‑235. No definitive rodent CEACAM-7 has been reported.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 7

Alternate Names

CEACAM7, CGM2

Entrez Gene IDs

1087 (Human)

Gene Symbol

CEACAM7

UniProt

Q14002 (Human)

Additional CEACAM-7 Products

Product Documents for Human CEACAM‑7 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CEACAM‑7 Biotinylated Antibody

For research use only

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