Human CEACAM-7 Biotinylated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CEACAM-7 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human CEACAM-1, -3, -4, -5,
    -6, and -8 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human CEACAM-7
    Asn37-Phe142
    Accession # Q14002
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human CEACAM‑7 by Western Blot. Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human CEACAM‑7 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4478) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CEACAM‑7 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CEACAM-7

CEACAM-7 (carcinoembryonic antigen cell adhesion molecule 7; also CGM2) is a 40‑45 kDa member of the CEACAM subfamily of the CEA family of proteins. It has a restricted expression pattern, being found on epithelium of the colon, appendix and pancreatic ducts. It is markedly down‑regulated in colon carcinoma. Mature human CEACAM-7 is a 209 amino acid (aa), GPI-linked glycoprotein. It contains one V-type (aa 36‑142) and one C2-type (aa 146‑233) Ig-like domain, plus a 21 aa C‑terminal propeptide. One alternate splice form exists that shows a deletion of aa 143‑235. No definitive rodent CEACAM-7 has been reported.

  • Long Name:
    Carcinoembryonic Antigen-related Cell Adhesion Molecule 7
  • Entrez Gene IDs:
    1087 (Human)
  • Alternate Names:
    Carcinoembryonic antigen CGM 2; Carcinoembryonic antigen CGM2; Carcinoembryonic antigen gene family member 2; Carcinoembryonic antigen related cell adhesion molecule 7; CEA; CEACAM 7; CEACAM7; CEACAM-7; CGM 2; CGM2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Biotinylated Control

Ctrl BAF020 1
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Flow F0040 7
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Streptavidin-Alkaline Phosphatase

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NorthernLights Streptavidin NL557

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NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
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NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
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Streptavidin-Allophycocyanin

Flow F0050 1
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Avidin-Fluorescein

Flow F0030 1
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Supplemental Cell Selection Products
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MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

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Supplemental ELISA Products
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Streptavidin-HRP

ELISA DY998 44
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EvenCoat Streptavidin Coated Plates

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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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