Human CEACAM-7 Antibody [Biotin]

R&D Systems | Catalog # BAF4478

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all CEACAM-7 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CEACAM-7
Asn37-Phe142
Accession # Q14002

Specificity

Detects human CEACAM-7 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human CEACAM-1, -3, -4, -5, -6, and -8 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human CEACAM-7 Antibody [Biotin]

Detection of Human CEACAM-7 antibody by Western Blot.

Detection of Human CEACAM‑7 by Western Blot.

Western blot shows lysates of HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human CEACAM-7 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4478) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CEACAM-7 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human CEACAM-7 Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: HT‑29 human colon adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CEACAM-7

CEACAM-7 (carcinoembryonic antigen cell adhesion molecule 7; also CGM2) is a 40‑45 kDa member of the CEACAM subfamily of the CEA family of proteins. It has a restricted expression pattern, being found on epithelium of the colon, appendix and pancreatic ducts. It is markedly down‑regulated in colon carcinoma. Mature human CEACAM-7 is a 209 amino acid (aa), GPI-linked glycoprotein. It contains one V-type (aa 36‑142) and one C2-type (aa 146‑233) Ig-like domain, plus a 21 aa C‑terminal propeptide. One alternate splice form exists that shows a deletion of aa 143‑235. No definitive rodent CEACAM-7 has been reported.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 7

Alternate Names

CEACAM7, CGM2

Entrez Gene IDs

1087 (Human)

Gene Symbol

CEACAM7

UniProt

Q14002 (Human)

Additional CEACAM-7 Products

Product Documents for Human CEACAM-7 Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CEACAM-7 Antibody [Biotin]

For research use only

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