Human CEACAM-7 Antibody

Name: Human CEACAM-7 Antibody
Brand: R&D Systems
SKU: AF4478-SP
Rating: 5 (1 reviews)

Catalog #: AF4478 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4478
AF4478-SP

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Detection of Human CEACAM‑7 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CEACAM-7 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CEACAM-7 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 15% cross-reactivity with recombinant human (rh) CEACAM-3, rhCEACAM-8, and rhCEACAM-1 is observed and less than 2% cross-reactivity with rhCEACAM-6 and rhCEACAM-5 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CEACAM-7
Asn37-Phe142
Accession # Q14002

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

3-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CEACAM‑7 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line, HeLa human cervical epithelial carcinoma cell line and human liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CEACAM-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4478) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CEACAM-7 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry

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CEACAM‑7 in Human Colon Cancer Tissue. CEACAM-7 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Sheep Anti-Human CEACAM-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4478) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-7

CEACAM-7 (carcinoembryonic antigen cell adhesion molecule 7; also CGM2) is a 40‑45 kDa member of the CEACAM subfamily of the CEA family of proteins. It has a restricted expression pattern, being found on epithelium of the colon, appendix and pancreatic ducts. It is markedly down‑regulated in colon carcinoma. Mature human CEACAM-7 is a 209 amino acid (aa), GPI-linked glycoprotein. It contains one V-type (aa 36‑142) and one C2-type (aa 146‑233) Ig-like domain, plus a 21 aa C‑terminal propeptide. One alternate splice form exists that shows a deletion of aa 143‑235. No definitive rodent CEACAM-7 has been reported.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 7

Entrez Gene IDs

1087 (Human)

Alternate Names

Carcinoembryonic antigen CGM 2; Carcinoembryonic antigen CGM2; Carcinoembryonic antigen gene family member 2; Carcinoembryonic antigen related cell adhesion molecule 7; CEA; CEACAM 7; CEACAM7; CEACAM-7; CGM 2; CGM2