Human CFTR R Domain Antibody

Catalog # Availability Size / Price Qty
MAB1660
MAB1660-SP
Human CFTR R Domain Antibody in Data
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Product Details
Citations (11)
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Human CFTR R Domain Antibody Summary

Species Reactivity
Human
Specificity
Detects human CFTR. Specifically recognizes in vitro synthesized CFTR, recombinant CFTR protein, and non-recombinant CFTR protein (1, 2).
Source
Monoclonal Mouse IgG1 Clone # 13-1
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
beta -galactosidase-coupled CFTR
Cys590-Lys830 (predicted)
Accession # P13569
Formulation
Supplied as a solution in PBS containing BSA.
*Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Human CFTR transfected cell line
Immunohistochemistry
8-25 µg/mL
See below
Immunoprecipitation
1-2 µg/106 cells
T84 human colon carcinoma cell line, see our available Western blot detection antibodies
Immunofluorescence

Cheng, S.H. et al. (1990) Cell 63:827.
Marino, C.R. et al. (1991) J. Clin. Invest. 88:712.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry CFTR antibody in Human Placenta by Immunohistochemistry (IHC-P). View Larger

CFTR in Human Placenta. CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR R Domain Monoclonal Antibody (Catalog # MAB1660) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane and cytoplasm of decidual cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: CFTR

Cystic fibrosis transmembrane conductance regulator (CFTR) is a multi-pass transmembrane protein that functions as a chloride channel. CFTR belongs to the ATP-binding cassette (ABC) superfamily. Mutations in CFTR cause the pulmonary disease, cystic fibrosis (CF). Specifically, deletion of phenyalanine at position 508 (DeltaF508-CFTR) results in a folding defect which impairs chloride channel function. The mechanism by which channel dysfunction relates to disease symptoms is a focus of intense research. CFTR dysfunction results in disruption of ion transport and subsequent blockage of airways by secreted mucus. CFTR may also play a role in the skeletal muscle atrophy and dysfunction that characterizes CF. In addition, CFTR-mediated chloride secretion underlies fluid accumulation and cyst growth in autosomal dominant polycystic kidney disease (ADPKD).

References
  1. Gregory, R.J. et al. (1990) Nature 347:328.
  2. Cheng, S.H. et al. (1990) Cell 63:827.
Long Name
Cystic Fibrosis Transmembrane Conductance Regulator
Entrez Gene IDs
1080 (Human)
Alternate Names
ABC35; ABCC7CF; ATP-binding cassette sub-family C member 7; ATP-binding cassette transporter sub-family C member 7; cAMP-dependent chloride channel; CFTR; CFTR/MRP; Channel conductance-controlling ATPase; cystic fibrosis transmembrane conductance regulator (ATP-binding cassettesub-family C, member 7); cystic fibrosis transmembrane conductance regulator; cystic fibrosis transmembrane conductance regulator, ATP-binding cassette(sub-family C, member 7); dJ760C5.1; EC 3.6.3; MRP7EC 3.6.3.49; TNR-CFTR

Product Datasheets

Citations for Human CFTR R Domain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Synergy of cAMP and calcium signaling pathways in CFTR regulation
    Authors: Z Bozoky, S Ahmadi, T Milman, TH Kim, K Du, M Di Paola, S Pasyk, R Pekhletski, JP Keller, CE Bear, JD Forman-Kay
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Mutating the Conserved Q-loop Glutamine 1291 Selectively Disrupts Adenylate Kinase-dependent Channel Gating of the ATP-binding Cassette (ABC) Adenylate Kinase Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) and Reduces Channel Function in Primary Human Airway Epithelia.
    Authors: Dong Q, Ernst S, Ostedgaard L, Shah V, Ver Heul A, Welsh M, Randak C
    J Biol Chem, 2015;290(22):14140-53.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. A novel chimeric adenoassociated virus 2/human bocavirus 1 parvovirus vector efficiently transduces human airway epithelia.
    Authors: Yan, Ziying, Keiser, Nicholas, Song, Yi, Deng, Xuefeng, Cheng, Fang, Qiu, Jianming, Engelhardt, John F
    Mol Ther, 2013;21(12):2181-94.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. Defective CFTR expression and function are detectable in blood monocytes: development of a new blood test for cystic fibrosis.
    Authors: Sorio C, Buffelli M, Angiari C, Ettorre M, Johansson J, Vezzalini M, Viviani L, Ricciardi M, Verze G, Assael BM, Melotti P
    PLoS ONE, 2011;6(7):e22212.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Identification of a bone marrow-derived epithelial-like population capable of repopulating injured mouse airway epithelium.
    Authors: Wong AP, Keating A, Lu WY, Duchesneau P, Wang X, Sacher A, Hu J, Waddell TK
    J. Clin. Invest., 2009;119(2):336-48.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  6. Human-specific cystic fibrosis transmembrane conductance regulator antibodies detect in vivo gene transfer to ovine airways.
    Authors: Davidson H, McLachlan G, Wilson A, Boyd AC, Doherty A, MacGregor G, Davies L, Painter HA, Coles R, Hyde SC, Gill DR, Amaral MD, Collie DD, Porteous DJ, Penque D
    Am. J. Respir. Cell Mol. Biol., 2006;35(1):72-83.
    Species: Ovine
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. Chloride channels in the small intestinal cell line IEC-18.
    Authors: Basavappa S, Vulapalli SR, Zhang H, Yule D, Coon S, Sundaram U
    J. Cell. Physiol., 2005;202(1):21-31.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  8. Protection of Cftr knockout mice from acute lung infection by a helper-dependent adenoviral vector expressing Cftr in airway epithelia.
    Authors: Koehler DR, Sajjan U, Chow YH, Martin B, Kent G, Tanswell AK, McKerlie C, Forstner JF, Hu J
    Proc. Natl. Acad. Sci. U.S.A., 2003;100(26):15364-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Mitochondria-rich cells as experimental model in studies of epithelial chloride channels.
    Authors: Willumsen NJ, Amstrup J, Mobjerg N, Jespersen A, Kristensen P, Larsen EH
    Biochim. Biophys. Acta, 2002;1566(1):28-43.
    Species: Xenopus
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Reciprocal protein kinase A regulatory interactions between cystic fibrosis transmembrane conductance regulator and Na+/H+ exchanger isoform 3 in a renal polarized epithelial cell model.
    Authors: Bagorda A, Guerra L, Di Sole F, Hemle-Kolb C, Cardone RA, Fanelli T, Reshkin SJ, Gisler SM, Murer H, Casavola V
    J. Biol. Chem., 2002;277(24):21480-8.
    Species: Amphibian
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  11. Localization of cystic fibrosis transmembrane conductance regulator in chloride secretory epithelia.
    Authors: Denning GM, Ostedgaard LS, Cheng SH, Smith AE, Welsh MJ
    J. Clin. Invest., 1992;89(1):339-49.
    Species: Human
    Sample Types: Cell Lysates
    Applications: IHC

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