Human CFTR R Domain Antibody

R&D Systems | Catalog # MAB1660

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Amphibian, Frog - Xenopus (African Clawed Frog), Hamster, Ovine

Applications

Validated:

Immunohistochemistry, Western Blot, Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 13-1
View all CFTR Primary Antibodies »

Product Specifications

Immunogen

beta -galactosidase-coupled CFTR
Cys590-Lys830 (predicted)
Accession # P13569

Specificity

Detects human CFTR. Specifically recognizes in vitro synthesized CFTR, recombinant CFTR protein, and non-recombinant CFTR protein (1, 2).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CFTR R Domain Antibody

CFTR antibody in Human Placenta by Immunohistochemistry (IHC-P).

CFTR in Human Placenta.

CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR R Domain Monoclonal Antibody (Catalog # MAB1660) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane and cytoplasm of decidual cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human CFTR R Domain Antibody

Application
Recommended Usage

Immunofluorescence

Cheng, S.H. et al. (1990) Cell 63:827.
Marino, C.R. et al. (1991) J. Clin. Invest. 88:712.

Immunohistochemistry

8-25 µg/mL
Sample: Immersion-fixed paraffin-embedded sections of human placenta

Immunoprecipitation

1-2 µg/106 cells
Sample: T84 human colon carcinoma cell line, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Human CFTR transfected cell line

Reviewed Applications

Read 4 reviews rated 4.5 using MAB1660 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

For liquid material, refer to CoA for concentration.

Formulation

Supplied as a solution in PBS containing BSA.
*Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CFTR

Cystic fibrosis transmembrane conductance regulator (CFTR) is a multi-pass transmembrane protein that functions as a chloride channel. CFTR belongs to the ATP-binding cassette (ABC) superfamily. Mutations in CFTR cause the pulmonary disease, cystic fibrosis (CF). Specifically, deletion of phenyalanine at position 508 (DeltaF508-CFTR) results in a folding defect which impairs chloride channel function. The mechanism by which channel dysfunction relates to disease symptoms is a focus of intense research. CFTR dysfunction results in disruption of ion transport and subsequent blockage of airways by secreted mucus. CFTR may also play a role in the skeletal muscle atrophy and dysfunction that characterizes CF. In addition, CFTR-mediated chloride secretion underlies fluid accumulation and cyst growth in autosomal dominant polycystic kidney disease (ADPKD).

References

  1. Gregory, R.J. et al. (1990) Nature 347:328.
  2. Cheng, S.H. et al. (1990) Cell 63:827.

Long Name

Cystic Fibrosis Transmembrane Conductance Regulator

Alternate Names

ABC35, ABCC7CF, ATP-binding cassette sub-family C member 7, ATP-binding cassette transporter sub-family C member 7, cAMP-dependent chloride channel, CFTR/MRP, Channel conductance-controlling ATPase, cystic fibrosis transmembrane conductance regulator, cystic fibrosis transmembrane conductance regulator (ATP-binding cassettesub-family C, member 7), cystic fibrosis transmembrane conductance regulator, ATP-binding cassette(sub-family C, member 7), dJ760C5.1, EC 3.6.3, MRP7EC 3.6.3.49, TNR-CFTR

Entrez Gene IDs

1080 (Human)

Gene Symbol

CFTR

UniProt

P13569

Additional CFTR Products

Product Documents for Human CFTR R Domain Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CFTR R Domain Antibody

For research use only

Citations for Human CFTR R Domain Antibody

Customer Reviews for Human CFTR R Domain Antibody (4)

4.5 out of 5
4 Customer Ratings
5 Stars
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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human CFTR R Domain Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Placental tissue
    Species: Human
    Verified Customer | Posted 04/14/2025
    Heat pretreatment in basic buffer Blocking normal horse sera primary Ab MAB1660 x25, 30 min anti-mouse HRP, 30min & DAB
    Human CFTR R Domain Antibody MAB1660
  • Human CFTR R Domain Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Human
    Verified Customer | Posted 07/18/2021
    Human CFTR R Domain Antibody MAB1660
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 21811577
    Species: Human
    Verified Customer | Posted 02/10/2015
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 21952168
    Species: Human
    Verified Customer | Posted 02/10/2015

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Protocols

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FAQs

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