Human CFTR R Domain Antibody

(11 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CFTR. Specifically recognizes in vitro synthesized CFTR, recombinant CFTR protein, and non-recombinant CFTR protein (1, 2).
  • Source
    Monoclonal Mouse IgG1 Clone # 13-1
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    beta -galactosidase-coupled CFTR
    Cys590-Lys830 (predicted)
    Accession # P13569
  • Formulation
    Supplied as a solution in PBS containing BSA.
    *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Human CFTR transfected cell line
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • Immunoprecipitation
    1-2 µg/106 cells
    T84 human colon carcinoma cell line, see our available Western blot detection antibodies
  • Immunofluorescence

    Cheng, S.H. et al. (1990) Cell 63:827.
    Marino, C.R. et al. (1991) J. Clin. Invest. 88:712.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
CFTR in Human Placenta. CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR R Domain Monoclonal Antibody (Catalog # MAB1660) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane and cytoplasm of decidual cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Shipping
    The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C, as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 to -70 °C under sterile conditions after opening.
Background: CFTR
Cystic fibrosis transmembrane conductance regulator (CFTR) is a multi-pass transmembrane protein that functions as a chloride channel. CFTR belongs to the ATP-binding cassette (ABC) superfamily. Mutations in CFTR cause the pulmonary disease, cystic fibrosis (CF). Specifically, deletion of phenyalanine at position 508 (DeltaF508-CFTR) results in a folding defect which impairs chloride channel function. The mechanism by which channel dysfunction relates to disease symptoms is a focus of intense research. CFTR dysfunction results in disruption of ion transport and subsequent blockage of airways by secreted mucus. CFTR may also play a role in the skeletal muscle atrophy and dysfunction that characterizes CF. In addition, CFTR-mediated chloride secretion underlies fluid accumulation and cyst growth in autosomal dominant polycystic kidney disease (ADPKD).
  • References:
    1. Gregory, R.J. et al. (1990) Nature 347:328.
    2. Cheng, S.H. et al. (1990) Cell 63:827.
  • Long Name:
    Cystic Fibrosis Transmembrane Conductance Regulator
  • Entrez Gene IDs:
    1080 (Human)
  • Alternate Names:
    ABC35; ABCC7CF; ATP-binding cassette sub-family C member 7; ATP-binding cassette transporter sub-family C member 7; cAMP-dependent chloride channel; CFTR; CFTR/MRP; Channel conductance-controlling ATPase; cystic fibrosis transmembrane conductance regulator (ATP-binding cassettesub-family C, member 7); cystic fibrosis transmembrane conductance regulator; cystic fibrosis transmembrane conductance regulator, ATP-binding cassette(sub-family C, member 7); dJ760C5.1; EC 3.6.3; MRP7EC 3.6.3.49; TNR-CFTR
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Synergy of cAMP and calcium signaling pathways in CFTR regulation
    Authors: Z Bozoky, S Ahmadi, T Milman, TH Kim, K Du, M Di Paola, S Pasyk, R Pekhletski, JP Keller, CE Bear, JD Forman-Kay
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. Mutating the Conserved Q-loop Glutamine 1291 Selectively Disrupts Adenylate Kinase-dependent Channel Gating of the ATP-binding Cassette (ABC) Adenylate Kinase Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) and Reduces Channel Function in Primary Human Airway Epithelia.
    Authors: Dong Q, Ernst S, Ostedgaard L, Shah V, Ver Heul A, Welsh M, Randak C
    J Biol Chem, 2015;290(22):14140-53.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. A novel chimeric adenoassociated virus 2/human bocavirus 1 parvovirus vector efficiently transduces human airway epithelia.
    Authors: Yan, Ziying, Keiser, Nicholas, Song, Yi, Deng, Xuefeng, Cheng, Fang, Qiu, Jianming, Engelhardt, John F
    Mol Ther, 2013;21(12):2181-94.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  4. Defective CFTR expression and function are detectable in blood monocytes: development of a new blood test for cystic fibrosis.
    Authors: Sorio C, Buffelli M, Angiari C, Ettorre M, Johansson J, Vezzalini M, Viviani L, Ricciardi M, Verze G, Assael BM, Melotti P
    PLoS ONE, 2011;6(7):e22212.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Identification of a bone marrow-derived epithelial-like population capable of repopulating injured mouse airway epithelium.
    Authors: Wong AP, Keating A, Lu WY, Duchesneau P, Wang X, Sacher A, Hu J, Waddell TK
    J. Clin. Invest., 2009;119(2):336-48.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  6. Human-specific cystic fibrosis transmembrane conductance regulator antibodies detect in vivo gene transfer to ovine airways.
    Authors: Davidson H, McLachlan G, Wilson A, Boyd AC, Doherty A, MacGregor G, Davies L, Painter HA, Coles R, Hyde SC, Gill DR, Amaral MD, Collie DD, Porteous DJ, Penque D
    Am. J. Respir. Cell Mol. Biol., 2006;35(1):72-83.
    Species: Ovine
    Sample Type: Whole Tissue
    Application: IHC Frozen
  7. Chloride channels in the small intestinal cell line IEC-18.
    Authors: Basavappa S, Vulapalli SR, Zhang H, Yule D, Coon S, Sundaram U
    J. Cell. Physiol., 2005;202(1):21-31.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  8. Protection of Cftr knockout mice from acute lung infection by a helper-dependent adenoviral vector expressing Cftr in airway epithelia.
    Authors: Koehler DR, Sajjan U, Chow YH, Martin B, Kent G, Tanswell AK, McKerlie C, Forstner JF, Hu J
    Proc. Natl. Acad. Sci. U.S.A., 2003;100(26):15364-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  9. Reciprocal protein kinase A regulatory interactions between cystic fibrosis transmembrane conductance regulator and Na+/H+ exchanger isoform 3 in a renal polarized epithelial cell model.
    Authors: Bagorda A, Guerra L, Di Sole F, Hemle-Kolb C, Cardone RA, Fanelli T, Reshkin SJ, Gisler SM, Murer H, Casavola V
    J. Biol. Chem., 2002;277(24):21480-8.
    Species: Amphibian
    Sample Type: Cell Lysates
    Application: IP
  10. Mitochondria-rich cells as experimental model in studies of epithelial chloride channels.
    Authors: Willumsen NJ, Amstrup J, Mobjerg N, Jespersen A, Kristensen P, Larsen EH
    Biochim. Biophys. Acta, 2002;1566(1):28-43.
    Species: Xenopus
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  11. Localization of cystic fibrosis transmembrane conductance regulator in chloride secretory epithelia.
    Authors: Denning GM, Ostedgaard LS, Cheng SH, Smith AE, Welsh MJ
    J. Clin. Invest., 1992;89(1):339-49.
    Species: Human
    Sample Type: Cell Lysates
    Application: IHC
Expand to show all 11 Citations
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